Identification of free-living Oophila amblystomatis (Chlorophyceae) from Yellow Spotted Salamander and Wood Frog breeding habitat

Phycologia ◽  
2015 ◽  
Vol 54 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Yuan Lin ◽  
C.D. Bishop
Keyword(s):  
Breeding Habitat ◽  
Wood Frog ◽  
Spotted Salamander ◽  
Free Living ◽  
Oophila Amblystomatis

scholarly journals Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

2020 ◽  
Vol 10 (9) ◽  
pp. 3040
Author(s):  
Nádia Correia ◽  
Hugo Pereira ◽  
Joana T. Silva ◽  
Tamára Santos ◽  
Maria Soares ◽  
...  
Keyword(s):  
Growth Performance ◽  
Protein Content ◽  
Dry Weight ◽  
High Protein ◽  
Rrna Gene ◽  
Scale Production ◽  
Free Living ◽  
Microalgal Biomass ◽  
High Protein Content ◽  
Oophila Amblystomatis

Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g·m−2·day−1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g·m−2·day−1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg·g−1 DW, followed by lutein with 5.37 mg·g−1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.

Older is Better: Beaver Ponds on Boreal Streams as Breeding Habitat for the Wood Frog

2006 ◽  
Vol 70 (5) ◽  
pp. 1360-1371 ◽  
Author(s):  
CAMERON E. STEVENS ◽  
CYNTHIA A. PASZKOWSKI ◽  
GARRY J. SCRIMGEOUR
Keyword(s):  
Breeding Habitat ◽  
Wood Frog ◽  
Beaver Ponds ◽  
Boreal Streams

Winter snowfall determines the occupancy of northern prairie wetlands by tadpoles of the Wood Frog (Lithobates sylvaticus)

2011 ◽  
Vol 89 (11) ◽  
pp. 1063-1073 ◽  
Author(s):  
David B. Donald ◽  
William T. Aitken ◽  
Carrie Paquette ◽  
Shaun S. Wulff
Keyword(s):  
Relative Abundance ◽  
Winter Precipitation ◽  
Breeding Habitat ◽  
Wood Frog ◽  
Severe Drought ◽  
Prairie Wetlands ◽  
Spring Precipitation ◽  
Lithobates Sylvaticus ◽  
Long Term Effect

In the northern plains of North America, the wetland breeding habitat of amphibians and their populations could be reduced by a change in climate that included decreased precipitation. To test this hypothesis, relative abundance of late-stage tadpoles of the Wood Frog ( Lithobates sylvaticus (LeConte, 1825)) was monitored from 1997 to 2010 during a wet–dry–wet cycle in 29 wetlands distributed throughout central Saskatchewan, Canada. The wetlands were dry for up to 7 consecutive years, and for a mean of 3.8 consecutive years. Consequently, tadpole occupancy of the wetlands was reduced to less than 40% for 5 consecutive years and none of the wetlands had tadpoles during the severe drought of 2001 and 2002. However, the drought had no observable long-term effect on either tadpole occupancy of wetlands or tadpole abundance. In 2007, 93% of the wetlands supported tadpoles, and in 2008 the highest mean relative abundance of tadpoles was recorded. Tadpole occupancy of wetlands was related to winter and spring precipitation (R2 = 0.84) with 67% of long-term variation in occupancy related to snowfall from November to February and 17% related to rainfall from March to June. Less than 45 mm of winter precipitation for 6 consecutive years would probably cause regional extinction of populations of the Wood Frog.

Patterns of bacterial diversity in embryonic capsules of the spotted salamander Ambystoma maculatum: an expanding view of a symbiosis

2021 ◽  
Vol 97 (10) ◽  
Author(s):  
Cory Bishop ◽  
Emil Jurga ◽  
Lori Graham
Keyword(s):  
Bacterial Diversity ◽  
Vascular Plant ◽  
Amplicon Sequencing ◽  
Pond Water ◽  
Ambystoma Maculatum ◽  
Breeding Habitat ◽  
Spatial And Temporal Variation ◽  
Egg Mass ◽  
Rrna Gene ◽  
Spotted Salamander

ABSTRACT The unicellular green alga, Oophila amblystomatis, populates egg capsules of the spotted salamander Ambystoma maculatum. This nutrient-exchange mutualism is widely perceived as a bipartite interaction, but the presence and contributing effects of bacteria to this symbiosis are unknown. We used standard cultivation techniques and amplicon sequencing of the V4/V5 region of 16S rRNA gene to identify and compare diversity of bacterial taxa in embryonic capsules with that in the aquatic breeding habitat. Our sampling regime allowed us to investigate diversity among individual capsules of an egg mass and between two ponds and sampling years. Capsules contain much lower diversity of bacteria than pond water, and spatial and temporal variation in intracapsular and pond bacterial diversity was observed. Despite this variation, sequences corresponding to species in the orders Burkholderiales and Oligoflexales were either prevalent or abundant, or both. Isolates most commonly recovered from capsules were closely related to species in the genus Herbaspirillum (Burkholderiaceae); other isolates were pseudomonads, but in all cases are closely related to known vascular plant-associated species. We conclude that, despite observed variation, there are bacterial taxa whose presence is held in common spatially and temporally among capsules and that the symbiosis between O. amblystomatis and A. maculatum may involve these taxa.

Effects of atrazine on egg masses of the yellow-spotted salamander (Ambystoma maculatum) and its endosymbiotic alga (Oophila amblystomatis)

2015 ◽  
Vol 206 ◽  
pp. 324-331 ◽  
Author(s):  
Leilan Baxter ◽  
Richard A. Brain ◽  
Alan J. Hosmer ◽  
Mohini Nema ◽  
Kirsten M. Müller ◽  
...  
Keyword(s):  
Ambystoma Maculatum ◽  
Spotted Salamander ◽  
Egg Masses ◽  
Endosymbiotic Alga ◽  
Oophila Amblystomatis

Ultrastructure of neuromuscular relationships in the canine heartworm (dirofilaria immitis)

1986 ◽  
Vol 44 ◽  
pp. 278-279
Author(s):  
W. L. Steffens ◽  
Nancy B. Roberts ◽  
J. M. Bowen
Keyword(s):  
Dirofilaria Immitis ◽  
Domestic Dogs ◽  
Structural Description ◽  
Pharmacological Effects ◽  
Free Living ◽  
Canine Heartworm ◽  
The World ◽  
Synaptic Region ◽  
Muscle Innervation ◽  
Insight Into

The canine heartworm is a common and serious nematode parasite of domestic dogs in many parts of the world. Although nematode neuroanatomy is fairly well documented, the emphasis has been on sensory anatomy and primarily in free-living soil species and ascarids. Lee and Miller reported on the muscular anatomy in the heartworm, but provided little insight into the peripheral nervous system or myoneural relationships. The classical fine-structural description of nematode muscle innervation is Rosenbluth's earlier work in Ascaris. Since the pharmacological effects of some nematacides currently being developed are neuromuscular in nature, a better understanding of heartworm myoneural anatomy, particularly in reference to the synaptic region is warranted.

Lead and lead toxicity in domestic and free living birds

2003 ◽  
Vol 32 (1) ◽  
pp. 3-13 ◽  
Author(s):  
N. De Francisco ◽  
N. De Francisco ◽  
N. De Francisco
Keyword(s):  
Lead Toxicity ◽  
Free Living
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