Fucales (Phaeophyceae) from Spain characterized by large-scale discontinuous nuclear DNA contents consistent with ancestral cryptopolyploidy

Phycologia ◽  
2010 ◽  
Vol 49 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Amelia Gómez Garreta ◽  
Ma Antonia Ribera Siguan ◽  
Noemi Salvador Soler ◽  
Jordi Rull Lluch ◽  
Donald F. Kapraun
Keyword(s):  
Large Scale ◽  
Nuclear Dna ◽  
Dna Contents ◽  
Nuclear Dna Contents

scholarly journals Nuclear DNA contents, rDNAs, and karyotype evolution in subgenus Vicia: III. The heterogeneous section Hypechusa

PROTOPLASMA ◽  
2006 ◽  
Vol 228 (4) ◽  
pp. 167-177 ◽  
Author(s):  
P. Caputo ◽  
M. Frediani ◽  
G. Venora ◽  
C. Ravalli ◽  
M. Ambrosio ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Karyotype Evolution ◽  
Dna Contents ◽  
Nuclear Dna Contents

Nuclear DNA Contents of Coreopsis nuecensoides and C. nuecensis (Asteraceae), a Progenitor-Derivative Species Pair

1984 ◽  
Vol 145 (2) ◽  
pp. 240-245 ◽  
Author(s):  
H. James Price ◽  
Daniel J. Crawford ◽  
Randall J. Bayer
Keyword(s):  
Nuclear Dna ◽  
Species Pair ◽  
Dna Contents ◽  
Nuclear Dna Contents

Flow cytometric analysis reveals different nuclear DNA contents in cultivated Fonio (Digitaria spp.) and some wild relatives from West-Africa

2007 ◽  
Vol 267 (1-4) ◽  
pp. 163-176 ◽  
Author(s):  
H. Adoukonou-Sagbadja ◽  
V. Schubert ◽  
A. Dansi ◽  
G. Jovtchev ◽  
A. Meister ◽  
...  
Keyword(s):  
West Africa ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Wild Relatives ◽  
Flow Cytometric ◽  
Dna Contents ◽  
Nuclear Dna Contents

Nuclear DNA content of three Eucalyptus species estimated by flow and image cytometry

2009 ◽  
Vol 57 (6) ◽  
pp. 524 ◽  
Author(s):  
Milene Miranda Praça ◽  
Carlos Roberto Carvalho ◽  
Carolina Ribeiro Diniz Boaventura Novaes
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Methodological Approach ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Eucalyptus Species ◽  
International Consensus ◽  
Mean Values ◽  
Dna Contents ◽  
Nuclear Dna Contents

Previous flow cytometry (FCM) analyses delivered nearly equal mean values of nuclear 2C DNA content for Eucalyptus grandis Hill ex Maiden and E. urophylla S. T. Blake (1.33 pg and 1.34 pg, respectively), whereas E. globulus Labill. presented distinct mean values (1.09, 1.13 and 1.40). These differences have been attributed to the different methodological approach, utilised plant cultivar and presence of intrinsic metabolic compounds that affect fluorochrome fluorescence. In the present study, a FCM and image cytometry (ICM) design, following international consensus criteria, were adopted to reassess the nuclear DNA contents of the above-mentioned Eucalyptus species. Statistical analyses revealed either similar or discrepant nuclear DNA contents, depending on the standard species used and whether FCM or ICM was employed. Our results demonstrated that 2C DNA values obtained by FCM and ICM were most uniform when Solanum lycopersicum was used as a standard. Moreover, the values obtained for E. grandis and E. urophylla were close, but differed as much as 24.63% in relation to previous data, and E. globulus proportionally varied up to 25%. New DNA content values are suggested for these eucalypt species.

scholarly journals Genome Sizes in Hepatica Mill: (Ranunculaceae) Show a Loss of DNA, Not a Gain, in Polyploids

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
B. J. M. Zonneveld
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Close Relationships ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Dna Contents ◽  
Asiatic Species ◽  
Nuclear Dna Contents ◽  
Allotetraploid Species

Genome size (C-value) was applied anew to investigate the relationships within the genus Hepatica (Ranunculaceae). More than 50 samples representing all species (except H. falconeri), from wild and cultivated material, were investigated. Species of Hepatica turn out to be diploid (), tetraploid ( ), and a possible pentaploid. The somatic nuclear DNA contents (2C-value), as measured by flow cytometry with propidium iodide, were shown to range from 33 to 80 pg. The Asiatic and American species, often considered subspecies of H. nobilis, could be clearly distinguished from European H. nobilis. DNA content confirmed the close relationships in the Asiatic species, and these are here considered as subspecies of H. asiatica. Parents for the allotetraploid species could be suggested based on their nuclear DNA content. Contrary to the increase in genome size suggested earlier for Hepatica, a significant (6%–14%) loss of nuclear DNA in the natural allopolyploids was found.

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