scholarly journals Hematology Profile and Liver Histopathology in Escherichia coli Infected Layers Treated with Combination of Phyllanthus ( Phyllanthus niruri L. ) and Turmeric ( Curcuma domestica )

2021 ◽  
Vol 39 (1) ◽  
pp. 55
Author(s):  
Sri Hartati ◽  
Tri Untari ◽  
Bambang Sutrisno ◽  
Ida Fitriana
Keyword(s):  
Alternative Therapy ◽  
Economic Loss ◽  
Liver Histology ◽  
Control Group ◽  
Phyllanthus Niruri ◽  
Liver Sample ◽  
Liver Histopathology ◽  
Important Health ◽  
Leukocyte Counts ◽  
One Way Anova

Colibacillosis a disease that can cause considerable economic loss, remains an important health problem. Phyllanthus (Phyllanthus niruri L) and turmeric (Curcuma domestica) are herbs that can be used as immunomodulators. This study was aimed to determine the level of safety of the combination of phyllanthus and turmeric on hematology profile and liver histopathology of layers with colibacillosis. The layers were assigned to the following of 5 groups: a) colibacillosis group without treatment, b) colibacillosis group with 500 mg/kg BW of phyllanthus, c) colibacillosis group with 300 mg/kg BW of turmeric, d) colibacillosis group with phyllanthus and turmeric combination (1:1), e) colibacillosis group with combination of phyllanthus and turmeric (1:2) . After 21 days of treatment, blood and liver sample were collected. The hematological profile (hemoglobin, hematocrit, erythrocyte and leukocyte counts) and liver histology were examined. The result were analyzed statistically by one-way ANOVA. The group that received phyllanthus had higher levels of hemoglobine, haematocrit and erythrocytes than the control group. However, no significant differences were found for the overall groups. Treatment with the combination of turmeric and phyllanthus for 21 days did not cause changes in the hematological profiles or liver histology, and therefor this herbal combination can be used as an alternative therapy for colibacillosis in layers.

scholarly journals EFFECT OF NATURE SOUND THERAPY ON THE LEVEL OF CORTISOL IN POSTPARTUM PRIMIPARA

2018 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
Yulinda Laska ◽  
Suryono Suryono ◽  
Melyana Nurul Widyawati ◽  
Ari Suwondo ◽  
Suryati Kusworowulan
Keyword(s):  
Music Therapy ◽  
Alternative Therapy ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Brain Wave ◽  
Anova Test ◽  
Sound Therapy ◽  
Cortisol Levels ◽  
One Way Anova

Background: Prevalence of postpartum blues for Asia between 26-85%, while the prevalence in Indonesia is 50-70%. Of all women postpartum can experience this is experiencing stress, almost 80% of primiparous moms experience feelings of sadness after childbirth. These stresses can trigger an increase in cortisol. Music raises changes in brain wave status and stress hormones. Nature Sound music is music that has a slow tempo and can cause feelings relaxed and comfortable.Objective: To examine the effect of the nature music therapy on on cortisol levels in postpartum primipara.Methods: This was a true experimental study with pretest-posttest control group design. The study was conducted in the postpartum ward in the General Hospital of Semarang from November 2016 to January 2017. There were 39 postpartum primipara recruited in this study using simple random sampling divided into three groups: 1) the experiment group who received the nature music therapy for 15 minutes, 2) the experiment group who received the nature music therapy for 30 minutes, and 3) the control group. One-way ANOVA test was performed for data analysis.Results: One-way anova test showed p-value 0.010 (<0.05), which indicated that there was a statistically significant effect of the nature sound therapy on the cortisol level in the postpartum primipara.Conclusion: There was a significant effect of the nature music therapy on the cortisol levels in postpartum primipara. Thus, the application of nature music therapy can be an alternative therapy especially for postpartum primipara who experience emotional stress, physical, anxiety, and fatigue.

Pengaruh Pemberian Ekstrak Rumput Laut Merah (Kappaphycus alvarezii) terhadap Kadar LDL Pada Tikus Putih (Rattus Norvegicus) Jantan Galur Wistar Yang Diberi Diet Tinggi Lemak

2018 ◽  
Vol 15 (2) ◽  
pp. 112
Author(s):  
MENTARI AMENDA SAPUTRI ◽  
HERIN SETIANINGSIH
Keyword(s):  
Rattus Norvegicus ◽  
Kappaphycus Alvarezii ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova ◽  
Test Control

<p class="Default">Penyakit kardiovaskular merupakan penyebab kematian nomor satu di dunia. Gaya hidup masyarakat terutama dalam mengkonsumsi diet yang tidak sehat dapat meningkatkan kadar LDL yang dapat menyebabkan  penyakit kardiovaskular. Rumput laut merah (<em>Kappaphycus alvarezii</em>)<em> </em>yang banyak dibudidayakan di Indonesia mengandung flavonoid dan triterpenoid yang diduga dapat menurunkan kadar LDL. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) <em> </em>terhadap kadar LDL pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak. Penelitian ini adalah penelitian eksperimental murni laboratorik dengan rancangan penelitian <em>Post Test Control Group Design. </em>Sampel yang digunakan adalah 24 ekor tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang dibagi ke dalam tiga kelompok: kelompok yang diberi diet standar selama 28 hari (K1), kelompok yang diberi diet tinggi lemak selama 28 hari (K2), dan kelompok yang diberi diet tinggi lemak selama 28 hari dan pada hari ke-15 sampai hari ke-28 diberi ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) dengan dosis 140mg/200grBB/hari (K3). Hasil analisis statistik <em>One Way Anova </em>menunjukkan bahwa terdapat perbedaan kadar LDL yang signifikan antara ketiga kelompok pada penelitian ini (p&lt;0,001). Kadar LDL pada K2 (=16,00±3,29) meningkat secara bermakna dibandingkan dengan K1 (=10,62±1,77). Sedangkan kadar LDL pada K3 (=6,88±2,42) menurun secara bermakna dibandingkan dengan K2. Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) berpengaruh terhadap kadar LDL darah pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak.</p><p><strong>Kata kunci</strong> : diet tinggi lemak, LDL, <em>Kappaphycus alvarezii</em></p>

PENGELUARAN KOLOSTRUM DENGAN PEMBERIAN PERAWATAN PAYUDARA DAN ENDORPHIN MASSAGE PADA IBU POST SEKSIO SESAREA

2019 ◽  
Vol 2 (1) ◽  
pp. 34-40
Author(s):  
Diah Evawanna Anuhgera ◽  
Eka Fitria Panjaitan ◽  
Desika Wali Pardede ◽  
Desika Wali Pardede ◽  
Nikmah Jalilah Ritonga ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cesarean Section ◽  
Alternative Therapy ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
A Value ◽  
Quasi Experimental ◽  
Breast Care ◽  
June July

Spending colostrum in post-cesarean section mothers is difficult in the first week postpartum. Efforts to overcome colostrum disruption can use non-pharmacological methods, namely breast care and endorphin massage. This study aims to determine the expenditure of colostrum by giving breast care and endorphin massage to post-cesarean mothers. This research is a quasi-experimental study with a nonequivalent design posttest control group design. This research was carried out in the midwifery room of the second floor of Grand Medistra Lubuk Pakam Hospital during the June-July 2019 period. There were 48 participants selected using purposive sampling, with 18 assigned in breast care, endorphin massage and combination of breast care and endorphin massage. The time of spending colostrum expenditure is assessed every day while the amount of colostrum expenditure is assessed after intervening. Analysis of the data used is one-way Annova. The results showed that the combination of breast care and endorphin massage was the most effective action in the time of spending colostrum and the amount of colostrum with a value of p = 0,000. The combination of breast care and endorphin massage could be applied as an alternative therapy post-cesarean mothers    

scholarly journals Benefits of Dog-Assisted Therapy in Patients with Dementia Residing in Aged Care Centers in Spain

2021 ◽  
Vol 18 (4) ◽  
pp. 1471
Author(s):  
Eva Vegue Parra ◽  
Jose Manuel Hernández Garre ◽  
Paloma Echevarría Pérez
Keyword(s):  
Scientific Evidence ◽  
Alternative Therapy ◽  
Group Versus ◽  
Aged Care ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Neuropsychiatric Inventory ◽  
Randomized Controlled ◽  
Institutionalized Patients ◽  
Experimental Group

(1) Background: Currently, the scientific evidence on the benefits of assisted therapy with dogs in dementia is not clear. In this study, we want to evaluate such benefits through a randomized controlled clinical trial in multiple centers across the country. (2) Methods: The participants were people over 65 years old with dementia, residing in senior centers in Spain (n = 334). The experimental group underwent assisted therapy with dogs based on the Comprehensive Cognitive Activation Program in Dementia, for 8 months, with weekly sessions of 45 min. Data were collected at the commencement, middle, and end of the program, to evaluate the aspects using the Mini-Examination Cognitive, the modified Bartell Index, the Cornell Scale for Depression in Dementia and the Neuropsychiatric Inventory. (3) Results: The results show significant improvements in the experimental group versus the control group in the affective (T1 = p 0.000; T2 = p 0.000) and behavioral (T1 = p 0.005; T2 = p 0.000) aspects, with the affective aspect displaying greater progress in participants with additional depressive (p = 0.022) or anxiety (p = 0.000) disorders, shorter institutionalization periods (r = −0.222, p = 0.004), and those undergoing complementary psychotherapy (p = 0.033) or alternative therapy (p = 0.011). (4) Conclusions: Dog therapy is effective in improving the affective and behavioral aspects of institutionalized patients with dementia.

scholarly journals A cross-sectional study of lung functions in traffic police personnel at work in Kathmandu Valley, Nepal

2015 ◽  
Vol 1 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Hari Sunder Shrestha ◽  
Ojashwi Nepal ◽  
Kishor Khanal ◽  
Bhoopinder Kumar Kapoor
Keyword(s):  
Cross Sectional Study ◽  
Control Group ◽  
Kathmandu Valley ◽  
Cross Sectional ◽  
Traffic Police ◽  
Police Personnel ◽  
Lung Functions ◽  
Function Test ◽  
Pollution Exposure ◽  
One Way Anova

BACKGROUND: The present study was aimed to assess pulmonary functions in the traffic police personnel (TPP) posted on traffic duty in Kathmandu valley, Nepal. METHODS: The study group consisted of 17 females and 89 males, constituting 16% and 84% of the total police personnel studied, respectively. In the control group of 25 individuals, 16% (n=4) were female and 84% (n=21) were male. Portable desktop spirometer was used for the pulmonary function test (PFT) measurements. RESULTS: It is seen that in females as compared to males, PFT parameters show a significant decrease. One-way ANOVA conducted to compare the effect of duration of air pollution exposure showed that there is a significant variation in PFT parameters among the groups. The exposure duration has significant effect on the PFT parameters. CONCLUSION: Greater the officers are engaged in traffic duty for years, greater is the decrement in their lung functions test. DOI: http://dx.doi.org/10.3126/acclm.v1i1.12315 Ann. Clin. Chem. & Lab. Med. 1(1) 2015: 42-48

scholarly journals Curcumin Decreased Oxidative Stress, Inhibited NF-κB Activation, and Improved Liver Pathology in Ethanol-Induced Liver Injury in Rats

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Suchittra Samuhasaneeto ◽  
Duangporn Thong-Ngam ◽  
Onanong Kulaputana ◽  
Doungsamon Suyasunanont ◽  
Naruemon Klaikeaw
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Early Stage ◽  
Control Group ◽  
Liver Pathology ◽  
Sprague Dawley ◽  
Hepatocyte Apoptosis ◽  
Sod Activity ◽  
Ethanol Group ◽  
Liver Histopathology

To study the mechanism of curcumin-attenuated inflammation and liver pathology in early stage of alcoholic liver disease, female Sprague-Dawley rats were divided into four groups and treated with ethanol or curcumin via an intragastric tube for 4 weeks. A control group treated with distilled water, and an ethanol group was treated with ethanol (7.5 g/kg bw). Treatment groups were fed with ethanol supplemented with curcumin (400 or 1 200 mg/kg bw). The liver histopathology in ethanol group revealed mild-to-moderate steatosis and mild necroinflammation. Hepatic MDA, hepatocyte apoptosis, and NF-κB activation increased significantly in ethanol-treated group when compared with control. Curcumin treatments resulted in improving of liver pathology, decreasing the elevation of hepatic MDA, and inhibition of NF-κB activation. The 400 mg/kg bw of curcumin treatment revealed only a trend of decreased hepatocyte apoptosis. However, the results of SOD activity, PPARγprotein expression showed no difference among the groups. In conclusion, curcumin improved liver histopathology in early stage of ethanol-induced liver injury by reduction of oxidative stress and inhibition of NF-κB activation.

Application of Hydroxyapatite scaffold from Portunus pelagicus on OPG and RANKL expression after tooth extraction of Cavia cobaya

2021 ◽  
pp. 4647-4651
Author(s):  
Michael Josef Kridanto Kamadjaja ◽  
Sherman Salim ◽  
Birgitta Dwitya Swastyayana Subiakto
Keyword(s):  
Tooth Extraction ◽  
Alveolar Bone ◽  
Control Group ◽  
Rankl Expression ◽  
Control Group Design ◽  
Portunus Pelagicus ◽  
Group Design ◽  
Post Test ◽  
Hydroxyapatite Scaffold ◽  
One Way Anova

Objective: This study was to determine OPG and RANKL expression after hydroxyapatite (HA) scaffold from crab shells (Portunus pelagicus) application in tooth socket of Cavia cobaya. Methods: This study was a post-test only control group design. Twenty four Cavia cobaya was divided into 4 groups. The lower left incisor was extracted and given a combination of HA and gelatin scaffold. Experimental animals were sacrificed on the 7th and 14th day. The amount of OPG and RANKL expression was calculated under a light microscope at 1000x magnification. The statistical analysis was done by One Way ANOVA Test and Tukey HSD. Results: Compared to other groups, the lowest and the highest level of OPG and RANKL were in P14 group. Conclusion: HA scaffold from crab shells (Portunus pelagicus) can increase OPG expression and decrease RANKL expression in the process of regenerating alveolar bone after tooth extraction.

scholarly journals Evaluation of (anti)genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test

2013 ◽  
Vol 49 (1) ◽  
pp. 135-148 ◽  
Author(s):  
Fernando Márlisson de Queiroz ◽  
Kayo Wanderson de Oliveira Matias ◽  
Mylena Mylana Freire da Cunha ◽  
Aline Schwarz
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Weight Gain ◽  
Micronucleus Test ◽  
Body Weight Gain ◽  
Tap Water ◽  
Control Group ◽  
Cytotoxic Activities ◽  
Phyllanthus Niruri ◽  
Polychromatic Erythrocytes

Phyllanthus niruri L. (Euphorbiaceae), known as "quebra-pedra" (Portuguese for "stonebreaker"), is an herb used for kidney disorders. In light of its frequent use by the population, the present study aimed to investigate the genotoxic, antigenotoxic and cytotoxic activities of a standardized P. niruri extract in bone marrow rats. Three groups of 12 animals were treated daily by gavage over a period of 30 days, with 50, 150 or 250 mg/kg of P. niruri extract aqueous solution. The control group (n = 12) received tap water. At the end of treatment (day 31), groups were divided into two minor subgroups (n=6/group) and received cyclophosphamide (50 mg/kg, i.p.) or saline 0.9% (i.p.). After 24 hours, we evaluated the frequency of micronucleated polychromatic erythrocytes for each animal (MNPCE) at 1000 PCE. Cytotoxicity was evaluated with the PCE/NCE ratio (NEC = normochromatic erythrocytes). General toxicity was assessed during treatment using the parameters of body weight gain, ration and water consumption. The dry extract did not provoke changes in body weight, weight gain, ration and water intake or changes in the frequency of MNPCE or cytotoxicity in bone marrow. We propose that the P. niruri extract used here showed no genotoxic, antigenotoxic and cytotoxic activities under the experimental conditions.

scholarly journals Status Kerentanan Aedes Aegypti Vektor Demam Berdarah Dengue di Kota Padang

2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.

scholarly journals PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP PENURUNAN KADAR KOLETEROL DARAH TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 151
Author(s):  
Ausich Singal ◽  
Edwin De Queljoe ◽  
Paulina Yamlean
Keyword(s):  
Rattus Norvegicus ◽  
Dose Group ◽  
T Test ◽  
Blood Cholesterol ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Sechium Edule ◽  
One Way Anova ◽  
Paired T Test

ABSTRACT This study aims to determine the effect of infusion of conjoined pumpkin leaves (Sechium edule) on reducing total blood cholesterol of male white rats (Rattus norvegicus). The subjects of this study were 15 male white rats with an average body weight of 200 grams which were divided into 5 groups, each group consisted of 3 mice. The method used is a laboratory experiment with a completely randomized design. The results were obtained from 2 measurements of blood cholesterol levels, namely measurements before and after treatment. The treatment begins with the provision of high-fat foods for 48 days. On the 49th day a blood cholesterol level was measured before treatment. Furthermore, treatment was given to each group, namely aquades in the negative control group, simvastatin in the positive control group, and squash leaves infusion with their respective doses in the dose group I (40%), the dose group II (20%), and the dose group III (10%). Measurement of cholesterol levels after treatment was carried out on day 54. Data were analyzed by Paired t-test and One Way ANOVA. The analysis showed that there were no significant differences between treatment groups. Judging from the change in average and percentage, 40% infusion dose of siamese pumpkin leaves gives the best reduction in cholesterol levels. Keywords: Cholesterol, pumpkin leaves, male white mouse infusion. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa daun labu siam (Sechium edule ) terhadap penuruan kolesterol darah total tikus putih jantan (Rattus norvegicus). Subjek penelitian ini berupa tikus putih jantan berjumlah 15 ekor dengan berat badan rata-rata 200 gram yang dibagi dalam 5 kelompok, masing-masing kelompok sebanyak 3 ekor. Metode yang digunakan yaitu eksperimen laboratorium dengan rancangan acak lengkap. Hasil penelitian diperoleh dari 2 kali pengukuran kadar koleterol darah yaitu pengukuran sebelum dan sesudah perlakuan. Perlakuan dimulai dengan pemberian makanan tinggi lemak selama 48 hari. Pada hari ke49 dilakukan pengukuran kadar kolesterol darah sebelum perlakuan. Selanjutnya diberikan perlakuan pada tiap kelompok yaitu aquades pada kelompok kontrol negatif, simvastatin pada kelompok kontrol positif, dan infusa daun labu siam dengan dosis masing-masing pada kelompok dosis I (40 %), kelompok dosis II (20 %), dan kelompok dosis III (10%). Pengukuran kadar kolesterol sesudah perlakuan dilakukan pada hari 54. Data diananlisis dengan Paired t-test dan One Way ANOVA. Hasil analisa menunjukan tidak terdapat perbedaan yang bermakna antar kelompok perlakuan. Dilihat dari perubahan rerataan dan presentase, dosis infusa  40% daun labu siam memberikan penurunan kadar koleterol terbaik. Kata kunci : Infusa daun Labu siam, kolesterol, tikus putih jantan.

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