scholarly journals Deteksi Staphylococcus aureus dan Staphylococcus sp. Secara Langsung Dari Susu Segar Kambing Peranakan Etawa dengan Teknik PCR

2020 ◽  
Vol 38 (2) ◽  
pp. 168
Author(s):  
Fatkhanuddin - Aziz ◽  
Fajar Budi Lestari ◽  
Sarah Nuraida S. ◽  
Endah Purwati ◽  
Siti Isrina Oktavia Salasia
Keyword(s):  
Staphylococcus Aureus ◽  
Nucleic Acid ◽  
23S Rrna ◽  
Food Borne ◽  
Spin Column ◽  
Nucleic Acid Purification

Genus Staphylococcus merupakan salah satu patogen bakteri penyebab mastitis yang menyebabkan kerugian ekonomi pada kambing Peranakan Etawa. Diantara Staphylococcus sp. yang dapat tumbuh dengan baik dalam susu segar, diketahui Staphylococcus aureus dapat membahayakan kesehatan manusia yang mengkonsumsi (food borne disease) karena kemampuannya dalam memproduksi enterotoksin yang tahan terhadap enzim pencernaan maupun pemanasan. Tujuan penelitian ini adalah mendeteksi Staphylococcus sp. dan S. aureus secara langsung dari susu kambing peranakan etawa dengan teknik PCR.Metode yang dilakukan adalah mengekstraksi DNA dari 60 sample susu segar dengan prinsip spin column-based nucleic acid purification dan kemudian dilakukan amplifikasi gen spesifik 23S rRNA Staphylococcus sp. dan S. aureus. Hasil PCR diketahui 37 (61%) sampel susu positif mengandung Staphylococcus sp. dan hanya 1 (1,6%) sampel mengandung S. aureus. Metode deteksi dengan PCR dapat digunakan untuk mendeteksi kontaminan Staphylococcus sp. dan S. aureus dengan waktu yang singkat

scholarly journals Filter paper-based spin column for low throughput nucleic acid purification

2018 ◽  
Author(s):  
Rui Shi ◽  
Ramsey S. Lewis ◽  
Dilip R. Panthee
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Filter Paper ◽  
Genomic Dna ◽  
Total Rna ◽  
Binding Material ◽  
Pcr Product ◽  
Spin Column ◽  
Nucleic Acid Purification ◽  
Commercial Kits

AbstractWe describe a method of recharging used spin column or assembling homemade spin column using filter paper as binding material for low throughput nucleic acid purification. We evaluated the efficiency of filter paper based spin columns in the purification of different type of nucleic acids. For instance, by following protocols of respective commercial kits, we found that filter paper to be a useful binding material for purification of many types of nucleic acids, including plant genomic DNA, plant total RNA, PCR product, and DNA from agarose gels. We also found that filter paper has a weak binding affinity to plasmid DNA in tested miniprep protocols. Also, we present the protocols of using filter paper recharged spin column or homemade spin column for low throughput purification of plant genomic DNA and plant total RNA with commercial kit buffer leftover and less expensive homemade buffer.

Fully Integrated Lab-on-a-Disc for Nucleic Acid Analysis of Food-Borne Pathogens

2014 ◽  
Vol 86 (8) ◽  
pp. 3841-3848 ◽  
Author(s):  
Tae-Hyeong Kim ◽  
Juhee Park ◽  
Chi-Ju Kim ◽  
Yoon-Kyoung Cho
Keyword(s):  
Nucleic Acid ◽  
Fully Integrated ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Nucleic Acid Analysis

A highly sensitive fluorescence biosensor for detection of Staphylococcus aureus based on HCR-mediated three-way DNA junction nicking enzyme assisted signal amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Chuyan Zhang ◽  
Zewei Luo ◽  
Mengfan Wu ◽  
Wei Ning ◽  
Ziyi Tian ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Safety ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Safety Control ◽  
Food Borne ◽  
Highly Sensitive ◽  
Fluorescence Biosensor ◽  
Highly Sensitive Detection ◽  
Nicking Enzyme

Sensitive and efficient monitoring of food-borne bacteria is of great importance for food safety control. Herein, a novel biosensor for highly sensitive detection of Staphylococcus aureus (S. aureus) was constructed...

scholarly journals Limited-Resource Preparable Chitosan Magnetic Particles for Extracting Amplification-Ready Zeptomole-Range Nucleic Acid from Complex Biofluid

2021 ◽  
Author(s):  
Sayantan Tripathy ◽  
Arunansu Talukdar ◽  
Goutam Pramanik ◽  
P. V. Rajesh ◽  
Souradyuti Ghosh
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Magnetic Particles ◽  
Limited Resource ◽  
Nucleic Acid Amplification ◽  
Acid Extraction ◽  
Analytical Sensitivity ◽  
Nucleic Acid Extraction ◽  
Spin Column

<b>Layman Summary: </b>Nucleic acid extraction is a key prerequisite for any nucleic acid amplification test (NAAT) or isothermal NAAT (iNAAT) based molecular diagnosis assays.<b> </b>Existing methods utilizes spin column system for nucleic acid extraction which are unsuitable for limited resource settings. Our work explores two methods for chitosan coated magnetic particle preparation that can be executed within 6 h from commonly available chemicals with nothing but a magnetic stirrer and water bath and doable by a minimally trained person. We will also investigated the compatibility of the extracted nucleic acid with downstream NAATs such as real time LAMP, colorimetric LAMP, and real time PCR. In the process, we established the analytical sensitivity of the overall method.<div><br><div><b>Characterization methods</b>: SEM, XRD, EDX, FT-IR</div><div><br></div><div><b>Bioanalytical methods:</b> Real time LAMP, Colorimetric LAMP, Real time PCR</div></div>

scholarly journals Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (20) ◽  
Author(s):  
Phil Oberacker ◽  
Peter Stepper ◽  
Donna Bond ◽  
Katharina Hipp ◽  
Timothy Hore ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Simple Synthesis ◽  
Nucleic Acid Purification ◽  
Paramagnetic Beads

Recent developments of magnetic beads for use in nucleic acid purification

1998 ◽  
Vol 816 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Peter R Levison ◽  
Stephen E Badger ◽  
Jon Dennis ◽  
Prit Hathi ◽  
Martin J Davies ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Magnetic Beads ◽  
Recent Developments ◽  
Nucleic Acid Purification
Sign in / Sign up

Export Citation Format

Share Document