scholarly journals Studi In-Vivo Ekstrak Daun Teh Hijau (Camellia Sinensis) sebagai Alternatif anti Bakteri Eschericia Coli pada Ayam Broiler

2019 ◽  
Vol 37 (2) ◽  
pp. 172
Author(s):  
Bambang Sutrisno ◽  
R. Wasito ◽  
Kurniasih Kurniasih ◽  
Sitarina Widyarini ◽  
Yuli Purwandari Kristianingrum ◽  
...  
Keyword(s):  
Green Tea ◽  
Broiler Chickens ◽  
Leaf Extract ◽  
Histopathological Examination ◽  
Green Tea Extract ◽  
E Coli ◽  
Group B ◽  
Tea Extract ◽  
Tea Leaf

The prevalence of colibasillosis  in chicken farms in Indonesia is very high, treatment using antibiotics is experiencing resistance, so it is necessary to look for alternatives to antibacterial. The study was aimed to determine the antibacterial effect of green tea leaf extract on broiler chickens infected with Eschericia coli by looking at the score of macroscopic lesions strengthened by histopathological examination, heterophile examination, plasma protein and fibrinogen. The research used 20 day old broilers (DOC) which were randomly divided into 4 groups, group A group B, group C and group D, each consisting of 5 DOC broilers. While maintaining ND and Gumboro vaccines on schedule like maintenance in general. At the age of 21 days all broilers in each group began to be treated as controls (Group A) without infecting E. coli and were not given 0,1g/ml  water extract of green tea leaves (Camillia sinensis). Group B, intratracheal-infected broilers with local strains of E.coli were 108 cells / ml according to 0,5  Mc Farland standard, and were not given green tea leaf extract. Group C, broilers infected by intratracheal with local strains of E. coli 108 cells / ml by 0,5 Mc Farland standard, and given to drink green tea leaf extract (Camillia sinensis) 0,1 g/ml and group D, broilers were given drinking green tea leaf extract (Camillia sinensis) 0,1g/ml. During the treatment all of chickens were given food and drink ad libitum. Fourteen days after infection of E.coli, 5 chickens in each group were collected to collect blood for heterophyll, total plasma protein (TPP) and fibrinogen. And then were euthanasied  with Mg SO4 saturated solution intravenously injection and necropsied  for gross and histpathological examination. Analysis of blood tests results were used one way of anova  (SPSS version 22 program), whereas for gross and histopathological examination with descriptive analysis. The results showed that the gross examination and histopathological organs of brolier infected with E. coli without being given a green tea extract experienced airsacculitis, pericarditis, perihepatitis and peritonitis, whereas broilers infected with E. coli and given green tea extract does not indicate the presence of inflammation. Examination of heterophile counts and blood fibrinogen levels had shown a difference (P <0.05), in broilers infected with E. coli and given green tea extracts had lower amounts of hetrophils and fibrinogen levels. While blood TPP levels were not significantly different (P> 0.05). The conclusion can be drawn, that the study of in vivo green tea extract (Camelia sinensis) 0,1g/ml has the potential to inhibit the infection of Eschericia coli bacteria in broiler chickens.

scholarly journals Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract

2021 ◽  
Vol 23 (1) ◽  
pp. 132-137
Author(s):  
O. Yu. Maslov ◽  
S. V. Kolisnyk ◽  
O. V. Hrechana ◽  
A. H. Serbin
Keyword(s):  
Dietary Supplements ◽  
Green Tea ◽  
Leaf Extract ◽  
Green Tea Extract ◽  
Biologically Active ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
Tea Extract ◽  
Tea Leaf ◽  
Active Substances

Recently, there has been a high demand from the population for dietary supplements that have antioxidant activity. First of all, these are dietary supplements with green tea leaf extract, because green tea contains catechins in a fairly high amount. Nowadays, according to the current legislation of Ukraine the qualitative composition and quantitative content of biologically active substances in dietary supplements are not established, which to a certain extent affects the quality and safety of these products. The aim of the study was to determine the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Materials and methods. Three dietary supplements from different manufacturers “Green Tea Extract”, “Green Tea Extract”, “Green Tea” were chosen for the study. Qualitative analysis was performed by thin layer chromatography, for quantification spectrophotometry in the UV-region was used. Results. The presence of catechins was found in the studied dietary supplements, caffeine was found only in trace amounts. The total content of catechins is 146.80 ± 1.36 mg, 79.00 ± 0.88 mg, 28.00 ± 0.75 mg, and in terms of green tea extract, which is stated in dietary supplements for dietary supplements “Green Tea Extract”, “Green Tea Extract”, “Green Tea”, respectively. Conclusions. Qualitative and quantitative analysis of some groups of BAS of three dietary supplements with green tea leaf extract was performed. It was found that dietary supplements “Green Tea Extract” and “Green Tea” meet the requirements of the US Pharmacopoeia 38 in terms of catechins. Based on the study, it can be concluded that the problem of compliance with dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

scholarly journals Prophylactic Catechin-Rich Green Tea Extract Treatment Ameliorates Pathogenic Enterotoxic Escherichia coli-Induced Colitis

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1573
Author(s):  
Jeong-Won Kim ◽  
Chang-Yeop Kim ◽  
Jin-Hwa Kim ◽  
Ji-Soo Jeong ◽  
Je-Oh Lim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Green Tea ◽  
Green Tea Extract ◽  
Intestinal Damage ◽  
Ki 67 ◽  
E Coli ◽  
Beneficial Effects ◽  
Tea Extract

In this study, we explored the potential beneficial effects of green tea extract (GTE) in a pathogenic Escherichia coli (F18:LT:STa:Stx2e)-induced colitis model. The GTE was standardized with catechin and epigallocatechin-3-gallate content using chromatography analysis. Ten consecutive days of GTE (500 and 1000 mg/kg) oral administration was followed by 3 days of a pathogenic E. coli challenge (1 × 109 CFU/mL). In vitro antibacterial analysis showed that GTE successfully inhibited the growth of pathogenic E. coli, demonstrating over a 3-fold reduction under time- and concentration-dependent conditions. The in vivo antibacterial effect of GTE was confirmed, with an inhibition rate of approximately 90% when compared to that of the E. coli alone group. GTE treatment improved pathogenic E. coli-induced intestinal injury with well-preserved epithelial linings and villi. In addition, the increased expression of annexin A1 in GTE-treated jejunum tissue was detected, which was accompanied by suppressed inflammation-related signal expression, including TNFA, COX-2, and iNOS. Moreover, proliferation-related signals such as PCNA, CD44, and Ki-67 were enhanced in the GTE group compared to those in the E. coli alone group. Taken together, these results indicate that GTE has an antibacterial activity against pathogenic E. coli and ameliorates pathogenic E. coli-induced intestinal damage by modulating inflammation and epithelial cell proliferation.

scholarly journals Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

2013 ◽  
Vol 49 (2) ◽  
pp. 341-349 ◽  
Author(s):  
Tariq Mahmood ◽  
Naveed Akhtar ◽  
Barkat Ali Khan ◽  
Akhtar Rasul ◽  
Haji M. Shoaib Khan
Keyword(s):  
Green Tea ◽  
Storage Conditions ◽  
Green Tea Extract ◽  
Viscosity Data ◽  
Skin Protection ◽  
Multiple Emulsion ◽  
Multiple Emulsions ◽  
Tea Extract

Complex multiple emulsions have an excellent ability to fill large volumes of functional cosmetic agents. This study was aimed to encapsulate large volume of green tea in classical multiple emulsion and to compare its stability with a multiple emulsion without green tea extract. Multiple emulsions were developed using Cetyl dimethicone copolyol as lipophilic emulsifier and classic polysorbate-80 as hydrophilic emulsifier. Multiple emulsions were evaluated for various physicochemical aspects like conductivity, pH, microscopic analysis, rheology and these characteristics were followed for a period of 30 days in different storage conditions. In vitro and in vivo skin protection tests were also performed for both kinds of multiple emulsions i.e. with active (MeA) and without active (MeB). Both formulations showed comparable characteristics regarding various physicochemical characteristics in different storage conditions. Rheological analysis showed that formulations showed pseudo plastic behavior upon continuous shear stress. Results of in vitro and in vivo skin protection data have revealed that the active formulation has comparable skin protection effects to that of control formulation. It was presumed that stable multiple emulsions could be a promising choice for topical application of green tea but multiple emulsions presented in this study need improvement in the formula, concluded on the basis of pH, conductivity and apparent viscosity data.

scholarly journals Antibacterial effect of green tea extract against multi drug resistant Escherichia coli isolated from urine sample of patients visiting tertiary care hospital of Eastern Nepal

2020 ◽  
Vol 8 (1) ◽  
pp. 45-51
Author(s):  
Kabita Giri ◽  
Bijay Kumar Shrestha ◽  
Jenish Shakya ◽  
Shiv Nandan Sah ◽  
Hemanta Khanal
Keyword(s):  
Escherichia Coli ◽  
Green Tea ◽  
Antibacterial Effect ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Green Tea Extract ◽  
Care Hospital ◽  
Drug Resistant ◽  
E Coli ◽  
Tea Extract

Urinary tract infections (UTIs) caused by drug resistant (DR) Uropathogenic Escherichia coli have become a significant worldwide public health problem. Green tea (Camellia sinensis), has been reported to have antimicrobial activities against various pathogenic bacteria. The main aim of our study was to estimate the antibacterial effect of green tea extract against drug resistant Uropathogenic E. coli isolated from urine samples of patients visiting in tertiary care hospital from eastern Nepal. During the study 360 urine samples were collected from UTI suspected patients visiting a tertiary care hospital of Biratnagar. Urine samples were cultured by using semi-quantitative culture technique and bacteria was identified by standard microbiological procedure. Antibiotic susceptibility testing was done by Kirby-Bauer Disk Diffusion method according to NCCLS (2011) guidelines. The antibacterial effect of green tea extract was performed by preparing the 95% ethanol extract in Soxhlet apparatus which was dispensed in DMSO solution and sterilized by membrane filtration. Antibacterial activity of Green Tea Extract against MDR Uropathogenic Escherichia coli was performed by making different concentration of green tea. The overall prevalence of E. coli was 27.22% in study population whereas the prevalence of MDR E. coli was 21.08%. All the isolated E. coli exhibited 100% sensitivity towards Nitrofurantoin and it was still a drug of choice for the treatment of Urinary tract infection caused by E. coli. The green tea extracts exhibited effective antibacterial activity against MDR E. coli. The MIC of Green Tea Extract was found to be 600µg/ml for 24 MDR isolates and 1000µg/ml for remaining 11 isolates. Based on the present study it is concluded that Green Tea extracts have great potential as an antimicrobial agent against E. coli. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 45-51

Effect of defined green tea extract in various dosage schemes on drug-metabolizing enzymes in mice in vivo

2014 ◽  
Vol 10 ◽  
pp. 327-335 ◽  
Author(s):  
Petra Matoušková ◽  
Hana Bártíková ◽  
Iva Boušová ◽  
Barbora Szotáková ◽  
Jan Martin ◽  
...  
Keyword(s):  
Green Tea ◽  
Green Tea Extract ◽  
Drug Metabolizing Enzymes ◽  
Metabolizing Enzymes ◽  
Tea Extract

scholarly journals Design and in vivo evaluation of emulgel formulations including green tea extract and rose oil

2013 ◽  
Vol 63 (4) ◽  
pp. 531-544 ◽  
Author(s):  
Evren Algin Yapar ◽  
Özge Ýnal ◽  
M. Sedef Erdal
Keyword(s):  
Green Tea ◽  
Barrier Function ◽  
Antioxidant Properties ◽  
Physicochemical Characterization ◽  
Green Tea Extract ◽  
In Vivo Studies ◽  
Skin Barrier Function ◽  
Rose Oil ◽  
Tea Extract

Abstract Prevention of skin aging and its treatment is an emerging field for development of new formulations in cosmetics. Accordingly, plant extracts with antioxidant properties are beneficial cosmetic ingredients for this purpose. This study was aimed at developing a stable and easily manufactured emulgel including green tea extract and rose oil that is effective on the barrier function and hydration of the skin. An emulgel formulation containing 20 % green tea extract and 5 % rose oil was designed as a result of pre-formulation studies. Physicochemical characterization, in vitro stability studies, in vivo water content of the stratum corneum and transepidermal water loss studies were carried out afterwards. In vivo studies on ten female subjects were evaluated by using non-invasive skin bioengineering techniques. Finally, a cosmetically acceptable, stable and effective emulgel formulation for skin barrier function with good hydrating properties was obtained for skin hydration, protection and anti-aging purposes.

scholarly journals To investigate the antimicrobial effects of extracted polyphenols from green tea (Camellia sinensis) and banana (Musa sp.) leaves

2015 ◽  
Author(s):  
Qin Xiang Ng
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Bacterial Infections ◽  
Bactericidal Effect ◽  
Green Tea Extract ◽  
Bacterial Strains ◽  
E Coli ◽  
Musa Sp ◽  
Banana Leaves ◽  
Tea Extract

In recent years, there is an emergence of antibiotic-resistant bacterial strains due to the indiscriminate use of antibiotics, commonly prescribed to treat infectious diseases. The increasing occurrence of antibiotic resistance has undoubtedly made bacterial infections a greater health risk and threat than before. In this project, the use of natural polyphenolic compounds, extracted from green tea (Camellia sinensis) and banana (Musa sp.) leaves, as a novel antimicrobial chemotherapeutic agent was investigated. This study examined their bactericidal effects on Escherichia coli and Micrococcus luteus, and also evaluated the potential synergistic ability of these polyphenol-rich extracts when used alongside the beta-lactam antibiotic ampicillin. For the same dry mass of plant material used (3g), the prepared green tea extract had a higher total polyphenol concentration than that of banana leaves extract (495.1mg/L as compared to 221.6mg/L respectively). Subsequently, bacterial broth of 105 colony forming units/ml was mixed with varying concentrations of the different test compounds before plating 10µl of the mixture and quantifying the compounds’ bactericidal effect by doing a viable count after overnight incubation. The green tea extract showed the greatest bactericidal effect on E. coli and M. luteus as compared to the banana leaves extract and ampicillin. Against E. coli, this difference was confirmed to be significant (p<0.05) using one-way ANOVA and Tukey’s post-hoc analysis. Furthermore, by applying ampicillin in close association with polyphenol-rich extracts of green tea or banana leaves, there was an enhanced bactericidal effect on the tested bacterial strains. This interaction was categorized as synergistic by the fractional bactericidal concentration index calculated (≤0.5). Ultimately, the plant polyphenols studied in this research could provide a potential adjuvant to currently-produced antibiotics and help to reduce antibiotic application concentrations; as well as prolong the effective lifespan of antibiotics through its synergistic action.

In Vivo and In Vitro Antitumor Effect of Ascorbic Acid, Lysine, Proline, Arginine, and Green Tea Extract on Human Fibrosarcoma Cells HT-1080

2006 ◽  
Vol 23 (1) ◽  
pp. 105-112 ◽  
Author(s):  
M. Waheed Roomi ◽  
Vadim Ivanov ◽  
Tatiana Kalinovsky ◽  
Aleksandra Niedzwiecki ◽  
Matthias Rath
Keyword(s):  
Ascorbic Acid ◽  
Green Tea ◽  
Antitumor Effect ◽  
Green Tea Extract ◽  
Fibrosarcoma Cells ◽  
Human Fibrosarcoma Cells ◽  
Tea Extract
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