scholarly journals Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani

2017 ◽  
Vol 35 (2) ◽  
pp. 254
Author(s):  
Teguh Budipitojo ◽  
Ariana ◽  
Tri Wahyu Pangestiningsih ◽  
Hery Wijayanto ◽  
Dwi Liliek Kusindarta ◽  
...  

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of  Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic  documentation of  Indonesia’s native biodiversity. 

Physiotherapy ◽  
2015 ◽  
Vol 101 ◽  
pp. e524-e525
Author(s):  
S. Kouda ◽  
T. Dakeshita ◽  
S. Hara ◽  
K. Wakimoto ◽  
Y. Uchida

2006 ◽  
Vol 189 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Susan Gray ◽  
Barbara S Stonestreet ◽  
Shanthie Thamotharan ◽  
Grazyna B Sadowska ◽  
Molly Daood ◽  
...  

We investigated the effects of maternal antenatal dexamethasone (Dex) treatment given as a single course (4 doses) or multiple courses (20 doses) on fetal skeletal muscle glucose transporter (GLUT) protein concentrations at 70% of gestation (106 to 107 days with term being 145 to 150 days) in the ovine fetus. Antenatal corticosteroid administration was associated with a decrease in endogenous fetal plasma cortisol concentrations (P < 0.05), fetal hyperglycemia (P < 0.02) and hyperinsulinemia (P < 0.05). These metabolic/hormonal changes were associated with a decrease in fetal body weight (P < 0.05) in the multiple course Dex group compared with the multiple course placebo group. These perturbations were associated with an increase in fetal skeletal muscle GLUT 1 concentrations that mediate basal glucose transport in the extensor digitorum lateralis and extensor digitorum longus muscles (P < 0.05) 18 h after the last dose of Dex was given in the single course group. However, in the multiple course Dex group, a small increase in GLUT 1 was observed only in the biceps femoris. In contrast, both single and multiple courses of antenatal Dex were associated with an increase in the extensor digitorum lateralis and biceps femoris muscle GLUT 4 (insulin-responsive) concentrations (P < 0.05). We conclude that antenatal corticosteroids perturb fetal glucose/insulin homeostasis, which is associated with increases in fetal skeletal muscle glucose transporters to compensate for and attenuate the associated catabolic fetal state. These changes consist of an increase in proteins that mediate basal glucose transport (GLUT 1) to meet immediate energy requirements of the fetal skeletal muscle with an increase in basal insulin sensitivity (GLUT 4) to compensate for the Dex-induced catabolic state after exposure to multiple courses of Dex.


Author(s):  
Miloslav Fabok ◽  
Bojan Leontijević ◽  
Lazar Tomić ◽  
Milivoj Dopsaj

The main aim of this study was to define the quantitative neuromuscular characteristics of Biceps Femoris muscle (BF) as the knee joint flexor, i.e. the major synergist of caudal body in all specific movement tasks of a football player, measured by tensiomyography (TMG) method . The secondary aim of the study was to compare all TMG-BF characteristics in relation to bilateral dimorphism, as well as to compare dominant and non-dominant legs. The research was conducted on a sample which included 54 professional players of age 23.0 ± 4.4 years; body height: 182.6 ± 15.1 cm; body mass: 81.2 ± 15.1 kg; BMI: 23.3 ± 1.2 kg/cm2. TMG variables were measured on the muscles during a transition training phase (mid-season and end of the season). The results have shown that the average Tc - 32.19 ± 7.64 and 33.21 ± 8.88 ms, Td - 25.56 ± 3.58 and 25.44 ± 3.20 ms, and Dm - 7.39 ± 1.87 and 7.52 ± 2.50 mm, for dominant and non-dominant leg, respectively. The results have indicated that there was no statistically significant difference between the examined TMG variables of dominant and non-dominant leg (Wilks' lambda Value = 0.979, F=0.300, p=0.952, Part. Ƞ2 = 0.021). It may be stated that there are no differences in the manifestation of neuromuscular characteristics in healthy elite football players regardless of their dominant leg. A high level of preparedness in football in addition to a completely healthy status of the body and locomotor system result in a complete neuromuscular contractile symmetry BF of both legs.


2004 ◽  
Vol 47 (4) ◽  
pp. 367-375 ◽  
Author(s):  
T. Kisiel ◽  
J. M. Książkiewicz

Abstract. In two Polish conservative flocks of Miniduck (K2) and Pekin (P33), registered by FAO as domestic genetic resources (World Watch List, 2000) the mean values of physical (tissue components) and qualitative traits of meat (pH15, pH24, colour of meat) were determined. The experimental flocks showed significant variations in body weight at 7 weeks of age (1540 vs 2088 in drakes and 1395 vs 1986 g in ducks), in the proportion of breast and leg muscles and skin with subcutaneous fat in eviscerated carcass. K2 ducks are characterized by outstanding musculature of breast and P33 ducks by that of lower leg and by low fatness. pH15 values of breast and leg muscles in K2 and P33 birds were greater than pH24 of these muscles. In both flocks pH15 values of 6.09 to 6.33 and pH24 values of 5.90 to 6.05 in breast muscles were lower than in leg muscles (6.33 to 6.46 and 6.12 to 6.37, respectively). A significantly lower L* value (darker colour) of breast muscle was characteristic of K2 birds (43.7 in males and 40.5 in females) compared to P33 males and females. Furthermore, breast muscles of K2 drakes and ducks had significantly higher redness (a*) and yellowness values (b*) than P33. In pectoralis superficialis muscle of K2 and P33 females, a significantly lower diameter was found for white muscle fibres (αW) (30.0 to 30.5 μm) and for red muscle fibres (βR) (16.9 to 17.6 μm) than in biceps femoris muscle (αW from 48.3 to 54.2; βR from 36.0 to 37.1 μm). Muscle fibres of K2 compared to P33 ducks were characterized by significantly greater diameters of βR muscle fibre in pectoralis superficialis muscle, and by lower αW and βR fibre diameters in biceps femoris muscle. The experiment showed that meat of ducks from the conservative flocks studied is valuable from the consumer’s point of view.


2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Gaku Kakehata ◽  
Yuta Goto ◽  
Shigeo Iso ◽  
Kazuyuki Kanosue

1994 ◽  
Vol 72 (1) ◽  
pp. 194-213 ◽  
Author(s):  
S. Falinower ◽  
J. C. Willer ◽  
J. L. Junien ◽  
D. Le Bars

1. Electromyographic recordings were made from the biceps femoris muscle through a pair of noninsulated platinum/iridium needle electrodes in male Sprague-Dawley rats artificially ventilated and anesthetized with 0.8% halothane in a N2O-O2 mixture (2/3:1/3). The animals' ventilation, heart rates, and body temperatures were continuously monitored. Electrical stimuli (2-ms duration, 0.16 Hz) were delivered to the sural nerve territory through a pair of noninsulated platinum/iridium needle electrode inserted subcutaneously in the medial aspect of the 4th and the lateral part of the 5th toe. Such stimulation elicited a two-component reflex response in the ipsilateral biceps femoris muscle: The first had a short latency (17.5 +/- 2.3 ms), short duration (20.7 +/- 2.6 ms), and low threshold (1.5 +/- 0.6 mA), whereas the second had a longer latency (162.4 +/- 5.1 ms), longer duration (202.3 +/- 6.2 ms), and higher threshold (5.7 +/- 0.5 mA). 2. Lidocaine (0.02–0.1%; 0.1 ml), but not saline, injected subcutaneously over the proximal part of the sural nerve, produced a selective depression of the late component of the reflex response, whereas the first component remained unchanged. The conduction velocity of the afferent fibers was estimated from the stimulation needles in the sural nerve territory to the nerve's projection in the lumbar spinal cord: it was concluded that the second, late component of the reflex response was due to afferent signals transmitted via unmyelinated C-fibers, whereas the first component was related to activation of fine myelinated fibers (A delta group). 3. Electrical stimulation of the sural nerve was still able to elicit the two-component reflex responses in the ipsilateral biceps femoris muscle of chronic spinal rats, indicating that these responses were genuine reflex responses, transmitted completely through a spinal circuit. 4. The C-fiber reflex was recorded when the duration and frequency of the stimuli applied to the sural nerve varied within the 0.5- to 4-ms and 0.02- to 1-Hz ranges, respectively. It was concluded that a single 2-ms duration shock at an intensity of 1.2 times the C-fiber reflex threshold, delivered every 6 s (0.16 Hz), constituted an acceptable and optimal protocol for experiments in which the C-fiber reflex was studied as a function of time. These parameters were used throughout the subsequent experiments.(ABSTRACT TRUNCATED AT 400 WORDS)


Medicine ◽  
2018 ◽  
Vol 97 (38) ◽  
pp. e12274 ◽  
Author(s):  
Jeong-Hyun Park ◽  
Kwang-Rak Park ◽  
Jinseo Yang ◽  
Gun-Hyun Park ◽  
Jaeho Cho

1996 ◽  
Vol 271 (1) ◽  
pp. H203-H211 ◽  
Author(s):  
H. Bitterman ◽  
V. Brod ◽  
G. Weisz ◽  
D. Kushnir ◽  
N. Bitterman

This study investigated mechanisms of the hemodynamic effects of oxygen in hemorrhagic shock induced by bleeding 30% of the total blood volume in anesthetized rats. An ultrasonic flowmeter was used to monitor regional blood flow. Changes in tissue perfusion were assessed by the laser-Doppler technique. The inhalation of 100% oxygen induced a significant increase in mean arterial blood pressure (MABP) and vascular resistance in the hindquarters, with a concomitant decrease in blood flow in the distal aorta and biceps femoris muscle. In contrast, oxygen did not change vascular resistance in the superior mesenteric artery (SMA) and renal beds and induced a significant increase in blood flow to the renal artery, SMA, and small bowel in hemorrhaged rats. L-Arginine (100 mg/kg iv) but not D-arginine or the vehicle (0.9% NaCl) completely abolished the effects of oxygen on blood pressure and reversed its effects on blood flow and resistance in the hindquarters and biceps femoris muscle. Administration of the nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (50 mg/kg iv) significantly increased MABP and the resistance in the three vascular beds. Pretreatment of hemorrhaged rats with a superoxide dismutase mimic, the NO-stable radical 2,2,6,6-tetramethylpiperidine-N-oxyl (5 mg/kg iv), resulted in significantly diminished effects of oxygen on hindquarter hemodynamics. These results demonstrate a differential effect of oxygen, which increases vascular resistance in the hindquarters without a significant effect in the splanchnic and renal beds, thus favoring an increase in splanchnic and renal perfusion. It is suggested that inactivation of NO by reactive oxygen species may underlie the effects of oxygen on hindquarter vascular tone during shock.


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