scholarly journals KAJIAN EQUINE INFECTIOUS ANEMIA PADA KUDA IMPOR DI BANDAR UDARA INTERNASIONAL SOEKARNO-HATTA

2018 ◽  
Vol 36 (1) ◽  
pp. 115
Author(s):  
Marlefzena Marlefzena ◽  
Sri Murtini ◽  
Joko Pamungkas
Keyword(s):  
Scientific Literature ◽  
Indirect Elisa ◽  
Animal Health ◽  
Health Information System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Initial Information ◽  
Blood Samples ◽  
Equine Infectious Anemia ◽  
The Relationship ◽  
Breed Horse

Equine Infectious Anemia (EIA) disease is caused by EIA Virus (EIAV) from genus Lentivirus, subfamily Orthoretrovirinae, family retroviridae. This virus causes a persistent infection and potentially fatal in Equidae. There is no scientific literature yet for this disease in Indonesia. In order to determine this disease in our country, it is necessary continuing laboratory detection in imported Equidae and conducted further surveillance in Equidae throughout Indonesia. This research is aimed (1) to detect of any antibodi titre of EIAV in imported horse, (2) To get information about the relationship between laboratory result with the secondary datas of origin country. The EIAV antibody detected by indirect enzyme-linked immunosorbent assay (indirect ELISA). The secondary datas were collected from Health Certificate of origin country, World Animal Health Information System (WAHIS) Interface OIE and literatures. Total of 133 blood samples were collected from imported horse, ex-imported horse and lokal breed horse. The result showed that all of imported horse, ex-imported horse and lokal breed horse were negative antibody against EIAV. The initial information on Equine Infectious Anemia indicated that health requirement in imported horse has fulfilled, so that imported horse was free from EIAV.

scholarly journals Serum biochemical properties of Vietnamese indigenous Noi chicken at 56 days old

2020 ◽  
Vol 36 (3) ◽  
pp. 329-339
Author(s):  
Nguyen Giang ◽  
Nguyen Hien ◽  
Huynh Loan ◽  
Phan Phuc ◽  
Nguyen Dai ◽  
...  
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Animal Health ◽  
Correlation Coefficients ◽  
Biochemical Properties ◽  
Blood Samples ◽  
Male And Female ◽  
Native Breed ◽  
Serum Biochemical ◽  
The Relationship

Serum profile is generally considered as comprehensive data which directly reflected animal health and their potential resistance to environmental, nutritional and pathological stress. The present study aimed to provide physiological reference values for selected biochemical parameters in Noi chickens, a famous Vietnamese native breed, at 56 days old. The collected blood samples of 355 Noi birds (164 males and 191 females) were used to evaluate biochemical serum profile. Glucose, total protein, albumin, globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine, and uric acid in the serum were measured. The variation in these values arising from different sexes was also investigated. The results indicated that there were no differences (P>0.05) in the assessed indices between male and female Noi chickens. In overall for the whole group, these values were obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The relationship among the biochemical parameters showed relatively low coefficients (r=0.79 in maximum). The females obtained higher correlation coefficients between levels of total protein and glucose (r=0.42) as well as between total protein and globulin (r=0.79), compared to the males. It was concluded that the male and female chickens performed similar values of serum indices. The contribution of this study might expand the knowledge on the biochemical profile and improved breeding strategies of Vietnamese indigenous Noi chickens.

scholarly journals Seroprevalence of ovine brucellosis in the microregion of Teresina, Piauí, Brazil

2020 ◽  
Vol 87 ◽  
Author(s):  
Letícia Soares de Araújo Teixeira ◽  
Joilson Ferreira Batista ◽  
Pedro Henrique Fonseca Silva ◽  
Maria Luiza Lima Cordeiro ◽  
Raissa Costa Amorim ◽  
...  
Keyword(s):  
Reproductive System ◽  
Indirect Elisa ◽  
Jugular Vein ◽  
Domestic Animals ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agar Gel ◽  
Serological Tests ◽  
Blood Samples ◽  
The Face

ABSTRACT: Among the diseases that affect the reproductive system of domestic animals, brucellosis in the sheep species is important because it generates significant economic losses to sheep rearing. Thus, it is a threat to the growth and productivity of sheep herds. In the face of this problem, the objective of the present research was to identify the prevalence of ovine brucellosis in herds in municipalities of the Teresina, Piauí, Brazil microregion by using the agar gel immunodiffusion assay (AGID) and indirect enzyme-linked immunosorbent assay (ELISA) serological tests. Fourteen municipalities were included in the research. Blood samples were collected from 521 pubescent animals by puncturing the jugular vein. After collection, the samples were submitted to the serological techniques, AGID and indirect ELISA, to detect anti B. ovis antibody. Of the 521 samples submitted to the AGIDtest, 23 (4.41%) were sera reagent and 498 (95.58%) negative. The indirect ELISA tests, 24 (4.61%) suspect samples and 497 (95.39%) negative samples were obtained, and there were no reagent animals in this test, only suspect. The seroprevalence of ovine brucellosis in the Teresina, Piauí microregion was 4.41%. Thus, it is possible to identify sheep with reagent serology to infection by B. ovis, that is present in municipalities in the state of Piauí, Brazil. Furthermore, AGIDwas shown to be more sensitive in detecting animals that had had contact with the etiological agent of the disease.

scholarly journals First serological detection of Borrelia spp. in dogs in western Cuba

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Matheus Dias Cordeiro ◽  
Claudia Bezerra da Silva ◽  
Maylin Gonzalez Navarrete ◽  
Eugênio Roque ◽  
Adivaldo Henrique da Fonseca
Keyword(s):  
Deoxyribonucleic Acid ◽  
Indirect Elisa ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Serological Detection ◽  
San Jose ◽  
Serum Samples ◽  
Blood Samples ◽  
Flab Gene

Abstract This study aimed to verify the presence of IgG antibodies against Borrelia burgdorferi sensu lato (s.l) in domestic dogs in western Cuba. Serum samples were analyzed by indirect enzyme-linked immunosorbent assay (ELISA), using crude antigens of a B. burgdorferi strain of North American origin. To verify the presence of Borrelia spp., deoxyribonucleic acid (DNA) extracted from individual blood samples was analyzed by nested-PCR, with markers targeted for amplification of portions of the flagellin B gene (flaB) present in Borrelia spirochetes. Ticks were also collected through inspection of the animals. Sera from 93 of 176 (52.84%) dogs were reactive to the indirect ELISA. Geographic prevalence varied from 54.35% (25/46) in Boyeros, 44.44% (20/45) in Cotorro, 66.67% (22/33) in Habana del Este, and 50% (26/52) in San José de las Lajas. There was no statistical difference between these tested variables. No blood samples analyzed were positive for the Borrelia flaB gene.

scholarly journals A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis

2019 ◽  
Vol 12 (6) ◽  
pp. 883-889 ◽  
Author(s):  
Marwa M. Attia ◽  
Elshaimaa Ismael ◽  
Nagla M. K. Saleh
Keyword(s):  
Early Diagnosis ◽  
Definitive Host ◽  
Gold Standard ◽  
Indirect Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Relation ◽  
Active Infection ◽  
Linguatula Serrata ◽  
Blood Samples ◽  
Concurrent Infections

Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats). This evaluation compared between the gold standard (GS) test (GS; examination of nasal and fecal samples in dogs and examination of lymph nodes in goats), sandwich enzyme-linked immunosorbent assay (S-ELISA), and indirect ELISA. Materials and Methods: Fifty goats and fifty dogs were examined for the presence of Linguatula serrata nymphs and adults, respectively, besides the collection of blood samples from the examined animals for serologic testing. Results: In goats; GS, S-ELISA, and indirect ELISA showed positivity in 32 (64%), 28 (56%), and 39 (78%) samples, respectively. In dogs; GS, S-ELISA, and indirect ELISA showed positivity in 25 (50%), 25 (50%), and 30 (60%) samples, respectively. S-ELISA displayed significant higher agreement with the GS test (≥0.83) than indirect ELISA (≤0.67) in both hosts. Infection with linguatulosis showed significant relation with the age of goats and dogs and the sex of goats (p<0.05). Conclusion: S-ELISA displayed more sensitivity and specificity for the detection of concurrent infections with linguatulosis in both dogs and goats than indirect ELISA, which could detect the prior infections. Similarly, these assays could be used for diagnosis of concurrent infections with linguatulosis in human, especially the chronic ones.

scholarly journals Increased IL-33 And IL-17 in Colorectal Carcinoma Patients with Severe Disease

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Veljko Maric ◽  
Milan Jovanovic ◽  
Natasa Zdravkovic ◽  
Marina Jovanovic ◽  
Nevena Gajovic ◽  
...  
Keyword(s):  
Liver Metastasis ◽  
Peritoneal Carcinomatosis ◽  
Poor Prognosis ◽  
Tumor Tissue ◽  
Liquid Fraction ◽  
Severe Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Advanced Stages ◽  
The Relationship

Abstract Colorectal cancer (CRC) represents one of the most common cancers. It is frequently diagnosed at advanced stages, indicating on need for new diagnostic markers. Th e aim of this study was to determine systemic and fecal values of IL- 17 and IL-33 in patients with CRC and the relationship with clinicopathological aspects of disease. Th e blood samples and feces liquid fraction of 50 patients with CRC were analyzed. Serum and fecal levels of IL-33 and IL-17 were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Fecal levels of IL-33 and IL-17 were increased in CRC patients with poor tumor tissue diff erentiation. Serum IL-33 and fecal IL-17 were increased in patients with presence of lung/liver metastasis or peritoneal carcinomatosis, respectively, while enhanced fecal IL-33 was detected only in patients with peritoneal carcinomatosis. Positive correlation between IL-33 and IL-17 values in sera and feces, respectively was also observed. We believe that increased local values of IL-33 and IL-17, reflected trough higher fecal concentration, in CRC patients with poor tumor tissue differentiation and with presence of lung/liver metastasis or peritoneal carcinomatosis may be considered as a sign of the tumor’s malignant progression and, consequently, of a poor prognosis for patients.

Development of an indirect ELISA based on a truncated S protein of the porcine epidemic diarrhea virus

2015 ◽  
Vol 61 (11) ◽  
pp. 811-817 ◽  
Author(s):  
Yufeng Li ◽  
Fangyuan Zheng ◽  
Baochao Fan ◽  
Hassan Mushtaq Muhammad ◽  
Yao Zou ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Indirect Elisa ◽  
Cross Reactivity ◽  
Virus Neutralization ◽  
Enzyme Linked Immunosorbent Assay ◽  
S Protein ◽  
Diarrhea Virus ◽  
Coefficients Of Variation ◽  
Porcine Epidemic Diarrhea ◽  
The Relationship

Porcine epidemic diarrhea (PED) is a highly contagious, enteric disease of swine caused by the porcine epidemic diarrhea virus (PEDV). To find a suitable ELISA method to assess the infection of PEDV and the effectiveness of vaccines, we developed and evaluated an indirect enzyme-linked immunosorbent assay (iELISA) based on a truncated recombinant spike (S) protein expressed in Escherichia coli. The parameters of the iELISA were optimized, and the cutoff value determined as 0.259 by analyzing optical density (OD) values of 80 PEDV negative sera confirmed by western blot. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were both less than 10%. Cross-reactivity assays demonstrated that iELISA was PEDV-specific. A virus neutralization test with sera of 7 different OD values showed a positive correlation between the OD values and virus neutralization. The results suggest this iELISA is specific, sensitive, and repeatable. Further studies should focus on the relationship between OD values of sera and its virus neutralization.

ISLAM DAN PANCASILA DALAM PEMIKIRAN MOHAMMAD NATSIR

2019 ◽  
Vol 3 (1) ◽  
pp. 82
Author(s):  
Mhd Alfahjri Sukri
Keyword(s):  
Political Parties ◽  
Qualitative Method ◽  
Political Socialization ◽  
Scientific Literature ◽  
Research Data ◽  
The Political ◽  
Direct Influence ◽  
Childhood Environment ◽  
Political Conditions ◽  
The Relationship

This research was conducted to see how Mohammad Natsir thought about the relationship between Islam and Pancasila. This study also explains the causes of Natsir's change of mind which initially supported Pancasila as part of Islam and later turned into an opponent of Pancasila in Konstituante on 11 November to 6 December 1957. The methodology used was a qualitative method by describing the results of the analysis carried out. The research data is obtained through a review of documents and scientific literature. The results of the study show that Mohammad Natsir's change of mind regarding the relationship between Islam and Pancasila was influenced by Mohammad Natsir's political socialization which began from Natsir's view of Islam influenced by the childhood environment (conditional and socio-cultural) in Minangkabau; direct influence from national figures such as Ahmad Hassan, H. Agus Salim, Sheikh Ahmad Syurkati and H. O Tjokroaminoto; the indirect influence of international figures throughout reading book such as Hassan Al-Banna, Amir Syakib Arselan, Rashid Ridha and Muhammad Abduh; the influence of Natsir's organization and political parties, namely Jong Islamieten Bond (JIB), Islamic Unity (Persis), and Masyumi political parties; and the influence of the political conditions at that time which made Natsir's views change, which initially accepted Pancasila and then became an opponent of the Pancasila. This research shown there are two patterns of Natsir's relationship with Pancasila, namely (1) Natsir accepted Pancasila and, (2) Natsir opposed Pancasila.

О НЕКОТОРЫХ ИСТОРИЧЕСКИХ АСПЕКТАХ СТАНОВЛЕНИЯ ВОСПИТАТЕЛЬНОЙ РАБОТЫ С ОСУЖДЕННЫМИ В МЕСТАХ ЛИШЕНИЯ СВОБОДЫ

2020 ◽  
Author(s):  
Vasiliy Dvortsov ◽  
Alexander Efimenko
Keyword(s):  
General Education ◽  
Scientific Literature ◽  
Criminal Code ◽  
The Self ◽  
Self Assessment ◽  
Educational Work ◽  
Legal Norms ◽  
Education Profession ◽  
Comprehensive Programs ◽  
The Relationship

В статье предпринята попытка теоретического анализа и изучения научной литературы по организации и становлению воспитательной работы с осужденными в местах лишения свободы, продемонстрирована взаимосвязь между политико-воспитательной работой, ресоциализацией и исправлением осужденных в пенитенциарных учреждениях. Проведенное исследование позволяет предполагать, что воспитательная работа является основополагающим средством исправления различных категорий осужденных (регламентировано ст. 9 УИК РФ). На этой основе критерием исправления будет становиться устойчивое правопослушное поведение человека. В связи с этим возникает необходимость использования психолого-педагогической программы по перестройке и самооценке осужденных, позволяющей формировать их готовность к самореализации, когда осознание совершенных преступлений становится внутренне неприемлемым. Авторами отмечается, что, самоисправление человека зависит от ряда направлений воспитательной работы: нравственного, правового, физического воспитания, получения основного общего образования, получения профессии. Очевидно, что для закрепления положительного результата процесс ресоциализации в пенитенциарных учреждениях должен проводиться сотрудниками всех отделов и служб на основе комплексных программ, разработанных с учетом специфики и возраста осужденных.The article attempts a theoretical analysis and study of scientific literature on the organization and formation of educational work with convicts in prisons, demonstrates the relationship between «political and educational work», re-socialization and correction of convicts in prisons. The study suggests that educational work is a fundamental means of correcting various categories of convicts (regulated by article 9 of the criminal code). Based on this criterion fixes will become sustainable human behavior, demonstrating a conscious rejection of the violation of legal norms with the aim of securing sustainable patterns of law-abiding behavior. There is a need to use the psychological and pedagogical Program for restructuring and self-assessment of convicts, which allows to form on this basis their readiness for self-realization, when the awareness of the crimes committed becomes internally unacceptable. In this regard, the self-correction of a person depends on a number of areas, namely, moral, legal, physical education, basic General education, profession, forming the basis of educational work. It is obvious that in order to consolidate a positive result in penitentiary institutions, the activities of all departments and services should be carried out a process of re-socialization on the basis of comprehensive Programs developed taking into account the specifics and different ages of convicts.

MiR-125a-5p Alleviates Dysfunction and Inflammation of Pentylenetetrazol- induced Epilepsy Through Targeting Calmodulin-dependent Protein Kinase IV (CAMK4)

2019 ◽  
Vol 16 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Qishuai Liu ◽  
Li Wang ◽  
Guizhen Yan ◽  
Weifa Zhang ◽  
Zhigang Huan ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Target Gene ◽  
Luciferase Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Factor ◽  
Dependent Protein Kinase ◽  
Expression Levels ◽  
Dependent Protein ◽  
Polymerase Chain ◽  
The Relationship

Background: MicroRNAs (miRNA) are known to play a key role in the etiology and treatment of epilepsy through controlling the expression of gene. However, miR-125a-5p in the epilepsy is little known. Epilepsy in rat models was induced by Pentylenetetrazol (PTZ) and miR- 125a-5p profiles in the hippocampus were investigated in our experiment. Also, the relationship between miR-125a-5p and calmodulin-dependent protein kinase IV (CAMK4) was identified and the related mechanism was also illustrated. Methods: The miR-125a-5p mRNA expression levels were evaluated by quantitative real time polymerase chain reaction (qRT-PCR). Western Blot (WB) was used to analyze the CAMK4 protein expression levels. Seizure score, latency and duration were determined based on a Racine scale. The enzyme-linked immunosorbent assay (ELISA) was used to analyze the inflammatory factor expression. The relationship between miR-125a-5p and CAMK4 was detected through dual luciferase assay. Results: Downregulation of miR-125a-5p was observed in the hippocampus of PTZ-induced epilepsy rats. The overexpression of miR-125a-5p attenuated seizure and decreased inflammatory factor level in the hippocampus of PTZ-induced rats. The miR-125a-5p alleviated epileptic seizure and inflammation in PTZ-induced rats by suppressing its target gene, CAMK4. Conclusion: miR-125a-5p may represent a novel therapeutic treatment for PTZ-induced epilepsy by preventing the activation of CAMK4.

scholarly journals Validation of a Novel Commercial ELISA Test for the Detection of Antibodies against Coxiella burnetii

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1075
Author(s):  
Salvatore Ledda ◽  
Cinzia Santucciu ◽  
Valentina Chisu ◽  
Giovanna Masala
Keyword(s):  
Diagnostic Accuracy ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Animal Health ◽  
Elisa Test ◽  
Clinical Diagnostics ◽  
Complex Life Cycle ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specificity And Sensitivity

Q fever is a zoonosis caused by Coxiella burnetii, a Gram-negative pathogen with a complex life cycle and a high impact on public and animal health all over the world. The symptoms are indistinguishable from those belonging to other diseases, and the disease could be symptomless. For these reasons, reliable laboratory tests are essential for an accurate diagnosis. The aim of this study was to validate a novel enzyme-linked immunosorbent assay (ELISA) test, named the Chorus Q Fever Phase II IgG and IgM Kit (DIESSE, Diagnostica Senese S.p.A), which is performed by an instrument named Chorus, a new device in medical diagnostics. This diagnostic test is employed for the detection of antibodies against C. burnetii Phase II antigens in acute disease. Our validation protocol was performed according to the Italian Accreditation Body (ACCREDIA) (Regulation UNI CEI EN ISO/IEC 17025:2018 and 17043:2010), OIE (World Organization for Animal Health), and Statement for Reporting Studies of Diagnostic Accuracy (STARD). Operator performance was evaluated along with the analytical specificity and sensitivity (ASp and ASe) and diagnostic accuracy of the kit, with parameters such as diagnostic specificity and sensitivity (DSp and DSe) and positive and negative predictive values (PPV and NPV), in addition to the repeatability. According to the evaluated parameters, the diagnostic ELISA test was shown to be suitable for validation and commercialization as a screening method in human sera and a valid support for clinical diagnostics.

Sign in / Sign up

Export Citation Format

Share Document