scholarly journals Protective Effect of Microbubble Aeration and Dietary Probiotics BALSS on Survival and Immunity of White Leg Shrimp (Litopenaeus vannamei) Postlarvae against Acute Low Salinity Stress

2020 ◽  
Vol 22 (1) ◽  
pp. 1
Author(s):  
Shima Bhaskara Ayuningrum ◽  
Indah Istiqomah ◽  
Rustadi Rustadi ◽  
Bambang Triyatmo ◽  
Alim Isnansetyo ◽  
...  
Keyword(s):  
Survival Rate ◽  
Glucose Level ◽  
Salinity Stress ◽  
Bactericidal Activity ◽  
Litopenaeus Vannamei ◽  
Stress Test ◽  
Stress Tests ◽  
Low Salinity ◽  
Before And After ◽  
Experimental Trials

Survival rate and immunity of white leg shrimp ((Litopenaeus vannamei) postlarvae against acute salinity stress were evaluated in indoor experimental trials. The research was conducted with 2x2 factorial design with two levels of aeration (microbubble and macro bubble) and two levels of diet (with and without probiotics) resulting four treatments in triplicate: application of microbubble with gut probiotics (MiP), without gut probiotics (MiC), and macro bubble with gut probiotics (MaP) and without probiotics (MaC). White leg shrimps measuring 7.6 g were maintained in the tanks for 60 days and fed five times a day at a dose of 5% biomass. Harvested shrimp were tested for low salinity stress (5 ppt) for 3 hours. Glucose level, total protein, bactericidal activity, natural agglutination, phenoloxidase, respiratory burst, and superoxide dismutase activities in the hemolymph and the shrimp survival rate (SR) were analyzed before and after the stress tests. Although all examined parameters remained the same between groups before the stress test, significant differences were found among them after the stress test. Microbubble aeration obtained stabilization of hemolymph glucose level, best survival rate, bactericidal activity, and agglutination activities. No significant effect was resulted by the dietary probiotic treatments, but the interaction with aeration treatments revealed the importance to compensate shrimp survival rate when microbubble aeration is unavailable.

Exercise performance in children and adolescents after the Ross procedure

2006 ◽  
Vol 16 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Bradley S. Marino ◽  
Sara K. Pasquali ◽  
Gil Wernovsky ◽  
John R. Bockoven ◽  
Michael McBride ◽  
...  
Keyword(s):  
Children And Adolescents ◽  
Exercise Performance ◽  
Stress Test ◽  
Ideal Body Weight ◽  
Exercise Stress Test ◽  
Exercise Stress ◽  
Ross Procedure ◽  
Stress Tests ◽  
Primary Indication ◽  
Before And After

Objectives:The Ross procedure is increasingly utilized in the treatment of aortic valvar disease in children and adolescents. Our purpose was to compare pre- and post-operative exercise state in this population.Methods:We included patients who underwent the Ross procedure at our institution between January, 1995, and December, 2003, and in whom we had performed pre- and post-operative exercise stress tests. We used a ramp bicycle protocol to measure consumption of oxygen and production of carbon dioxide. Cardiac output was estimated from effective pulmonary blood flow by the helium acetylene re-breathing technique.Results:We studied 26 patients, having a median age at surgery of 15.7 years, with a range from 7.5 to 24.1 years. The primary indication for surgery in two-thirds was combined aortic stenosis and insufficiency. Median time from the operation to the post-operative exercise stress test was 17.4 months, with a range from 6.7 to 30.2 months. There was a trend toward lower maximal consumption of oxygen after the procedure, at 36.3 plus or minus 7.6 millilitres per kilogram per minute (83.9% predicted) as opposed to 38.6 plus or minus 8.4 millilitres per kilogram per minute (88.5% predicted, p equal to 0.06). Patients after the procedure, however, had significantly increased adiposity, so that there was no difference in maximal consumption of oxygen indexed to ideal body weight before and after the operation. In 20 of the patients, aerobic capacity improved or was stable after the operation. There was no post-operative chronotropic impairment.Conclusions:In the majority of patients following the Ross procedure, exercise performance is stable and within the normal range of a healthy age and sex matched population, despite sedentary lifestyles and increased adiposity.

scholarly journals Substitution time of natural food by artificial diet on survival rate and growth of pacific white shrimp (Litopenaeus vannamei) postlarvae during rearing in low salinity media

2011 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Ferdinand Hukama Taqwa ◽  
Daniel Djokosetiyanto ◽  
Ridwan Affandi
Keyword(s):  
Survival Rate ◽  
Litopenaeus Vannamei ◽  
Crude Protein ◽  
Artificial Diet ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Natural Food ◽  
Daily Growth ◽  
Growth And Survival ◽  
Low Salinity

<p>This research was conducted to determine natural food substitution time by artificial diet   after salinity acclimatization from 20 ppt until 2 ppt, which can increase survival and growth of (<em>Litopenaeus vannamei</em>) postlarvae during rearing period. Design experiment was completely randomized design with five treatments and three replications of natural food <em>Chironomus</em> sp.  (60% of crude protein) substitution time by artificial diet (40% of crude protein) at day: 1 (A), 7 (B), 14 (C), 21 (D) and full natural food without artificial diet (E) during 28 days rearing period. White shrimp postlarvae and rearing media in this experiment based from best result of earlier research that is PL<sub>25 </sub>from acclimatization in media 2 ppt with addition of potassium 25 ppm to freshwater media.  The densities of PL<sub>25</sub> white shrimp were 20 PLs/50 liters of 2 ppt media. The result of this experiment showed that the use of artificial diet as soon as after salinity acclimatization (PL<sub>25</sub>) gave best performance production compared to which only that was given natural food <em>Chironomus</em> sp. during experiment or with treatment by artificial diet substitution at day-7, day-14 or day-21, shown with the highest value of food consumption level, protein retention, energy retention, daily growth rate and food efficiency. Survival rate of PL<sub>54</sub> was above 80% and not significant different between treatment. That is supported by chemical-physical value of water quality still in range appropriate to survival rate of white shrimp post larvae during a rearing period. The result of this experiment indicated that requirement nutrient of PL<sub>25</sub> in low salinity did not fulfilled if only rely on natural food, so that require artificial diet with nutrition content to support growth and survival rate of white shrimp post larvae more maximal.</p> <p>Key words: salinity, natural food, artificial diet, Pacific white shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tujuan penelitian ini adalah untuk menentukan waktu penggantian pakan alami oleh pakan buatan yang tepat selama masa pemeliharaan postlarva udang vaname di media bersalinitas rendah setelah melalui masa aklimatisasi penurunan salinitas dari 20 ppt hingga 2 ppt, sehingga dapat meningkatkan pertumbuhan dan kelangsungan hidup. Rancangan percobaan berupa rancangan acak lengkap dengan perlakuan yang diterapkan berupa waktu penggantian pakan alami <em>Chironomus </em>sp. (kadar protein 62%) oleh pakan buatan (kadar protein 40%) pada hari ke-1 (A), ke-7 (B), ke-14 (C), ke-21 (D) dan pakan alami (E) selama masa pemeliharaan. Postlarva udang vaname dan media pemeliharaan yang dipergunakan selama percobaan mengacu pada hasil terbaik yang didapatkan dari penelitian pendahuluan yaitu berupa PL<sub>25 </sub>hasil aklimatisasi di media bersalinitas 2 ppt  dengan penambahan kalium 25 ppm ke media air tawar pengencer. Padat tebar sebanyak 20 ekor/50 liter/wadah. Hasil percobaan menunjukkan bahwa pemberian pakan buatan yang diberikan segera setelah masa aklimatisasi salinitas (pada awal pemeliharaan PL<sub>25</sub>) memberikan performa produksi budidaya terbaik bila dibandingkan dengan yang hanya diberi pakan alami selama masa pemeliharaan maupun waktu penggantian pakan alami oleh pakan buatan pada hari ke-7, ke-14 dan hari ke-21 yang ditunjukkan dengan tingkat konsumsi pakan, retensi protein, retensi energi, laju pertumbuhan harian dan efisiensi pakan yang tertinggi.  Kelangsungan hidup di akhir pemeliharaan (PL<sub>54</sub>)  di atas 80% dan tidak berbeda nyata antar perlakuan. Hal ini ditunjang oleh nilai fisika kimia air yang berada dalam kisaran yang layak selama masa pemeliharaan. Hasil percobaan ini menunjukkan bahwa kebutuhan nutrisi pada stadia PL<sub>25</sub> di media bersalinitas rendah tidak terpenuhi jika hanya mengandalkan pakan alami sehingga perlu ditunjang dari pakan buatan dengankandungan nutrisi yang dapat mendukung pertumbuhan dan kelangsungan hidup yang lebih maksimal.</p> <p>Kata kunci: salinitas, pakan alami, pakan buatan, udang vaname.</p>

scholarly journals Effects of Nitrogen Sources on the Growth and Biochemical Composition of Diatom (Amphora coffeaeformis) Used for Shrimp Larviculture

2021 ◽  
Vol 18 (4) ◽  
Author(s):  
Chairat KHWANCHAROEN ◽  
Ninlawan CHOTIPAN ◽  
Thongchai NAWATMAI ◽  
Sataporn DIREKBUSSARAKOM
Keyword(s):  
Survival Rate ◽  
Ammonium Sulfate ◽  
Salinity Stress ◽  
Sodium Nitrate ◽  
Biochemical Composition ◽  
Water Exchange ◽  
Stress Test ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Nitrate Treatment

Amphora coffeaeformis is a benthic diatom and serves as a rich nutritional feed for various aquaculture industries. The objective of this experiment was to compare the effects of different nitrogen sources on the growth and biochemical composition of the diatom (A. coffeaeformis) culture. Sodium nitrate in Guillard’s f medium, used as a control treatment, was compared with sodium nitrite and ammonium sulfate. The results in the 3rd batch of culture showed that all treatments led to the highest growth on Day 6. The highest cell dry weight occurred in the ammonium sulfate treatment with significance (0.78±0.06 mg/mL). The cultures in each treatment were harvested at the exponential phase (Day 3) for biochemical composition analysis. A. coffeaeformis in the ammonium sulfate treatment significantly accumulated the highest protein content, at 44.82 % by dry weight, while the highest lipid content at 29.78 % by dry weight was significantly found in the sodium nitrate treatment. The cell size and structure showed no significant differences among the treatments. The experiment demonstrated the effects of different nitrogen sources on the growth and biochemical composition of A. coffeaeformis. After that, we selected A. coffeaeformis that was cultured with the nitrate and ammonia nitrogen sources for feeding to shrimp larvae. This experiment was conducted to evaluate the effects of A. coffeaeformis on the growth, survival rate, salinity stress test, and ammonia stress test for shrimp. The experiment was designed with four treatments, as follows: 1) the control feed with flake winner (no water exchange for 28 days), 2) Am-NO3 supplement with A. coffeaeformis cultured using nitrates (no water exchange for 28 days), 3) Am-NH3 supplement with A. coffeaeformis cultured using ammonia (no water exchange for 28 days), and 4) the positive control feed with flake winner (daily water exchange at 20 %). The results showed that the growth of shrimp fed with the diet supplemented with A. coffeaeformis cultured using nitrate and ammonia were not significantly different from the negative and positive controls (P > 0.05). However, the survival rate in the negative control was significantly lower than the other groups (P < 0.05). In this study, the stress test for white shrimp postlarvae utilized 2 methods: the ammonia or salinity stress tests. The survival rate of the shrimp after the ammonia stress test was not statistically and significantly different (P > 0.05). However, the results from the salinity stress test indicated that the highest survival rate was observed in the shrimp fed with the diet supplemented with A. coffeaeformis cultured using nitrate. A. coffeaeformis cultured with the sodium nitrate treatment had a positive effect on shrimp tolerance to salinity changes.

Investigation of Forward Voltage Degradation due to Process-Induced Defects in 4H-SiC MOSFET

2018 ◽  
Vol 924 ◽  
pp. 365-368 ◽  
Author(s):  
Kumiko Konishi ◽  
Ryusei Fujita ◽  
Yuki Mori ◽  
Akio Shima
Keyword(s):  
High Reliability ◽  
Stress Test ◽  
Implantation Dose ◽  
Imaging Method ◽  
Stress Tests ◽  
Forward Voltage ◽  
Current Stress ◽  
Before And After ◽  
Induced Defects ◽  
Voltage Degradation

We investigated process induced defects at various ion implantation conditions, and evaluated forward voltage degradation of body diode in 3.3 kV SiC MOSFET. First, by using photoluminescence (PL) observation, we evaluated the formation level of Basal Plane Dislocations (BPD) induced by Al implantation and anneal process with various Al implantation dose. Second, 3.3 kV double-diffused SiC MOSFETs were fabricated and forward current stress tests were performed to body diodes in SiC MOSFETs. Then, electrical characteristics of SiC MOSFETs before and after the stress test were measured, and expanded Stacking faults (SFs) in SiC epitaxial layer after the stress test were observed by PL imaging method. These results indicate that low dose or high temperature Al implantation conditions can suppress the formation of BPDs, and SiC MOSFETs fabricated using optimized Al implantation conditions show high reliability under current stress test.

Salinity stress test as a predictor of survival during growout in pacific white shrimp (Litopenaeus vannamei)

Aquaculture ◽  
2004 ◽  
Vol 237 (1-4) ◽  
pp. 237-249 ◽  
Author(s):  
Alma L Álvarez ◽  
Ilie S Racotta ◽  
Olivia Arjona ◽  
Elena Palacios
Keyword(s):  
Salinity Stress ◽  
Litopenaeus Vannamei ◽  
Stress Test ◽  
Pacific White Shrimp ◽  
White Shrimp
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