scholarly journals Determination of Aflatoxins in Feed by HPLC and PCR

2020 ◽  
pp. 315-323
Author(s):  
Areej Zuhair Azeez ◽  
Mrwa Thamer Hindi ◽  
Maha Muhamed Khudiar ◽  
Adel Saadi AL Saadi ◽  
Noor Ibrahim Khadim

The aim of this study is to identify aflatoxin secretion isolates in animal feeds by using HPLC and PCR  methods . In this study we collected fourty three samples of animal feed from different sites in Iraq (maize ,soybean ,sunflower grain ,barley grain, wheat). we isolated fungi  on potato dextrose agar, Aspergillus flavus  fungi was isolated from this samples  and identified the enzyme activities were tested for this isolate. The detection and determination for aflatoxin secretion of the isolates were done by using High Performance Liquid Chromatography (HPLC) technique. Twelve isolates shown Aflatoxin B1 secretion. Polymrease chain reaction ( PCR) technique is an alternative method to detect for Aspergillus spp. strains that secret aflatoxin by using specific primers( ITS1) endogenous gene for  Aspergillus flavus  and (ord , nor) genes  for aflatoxin B1 secreation, the PCR technique  considered to be an important role for safety and quality in industrial food and feed.

Author(s):  
А.А. ГРЯЗНОВ ◽  
Ю.А. ЛЕТЯГО ◽  
О.А. ГРЯЗНОВА ◽  
Р.И. БЕЛКИНА

Исследовано влияние добавки микроводоросли спирулины в рецептуру хлеба из пшеничной муки, обогащенного обойной мукой из зерна ячменя сортов Гранал 32 и Нудум 95 и тритикале сорта Цекад 90, на показатели его качества. Размолотую до порошкообразного состояния спирулину в количестве 0,5 к массе муки вносили при замесе теста в три опытных образца: пшеничный хлеб, хлеб Гранал и Ясень , рецептуры которых были разработаны ранее. В качестве контрольного образца был хлеб из пшеничной муки высшего сорта без добавок. Лабораторную выпечку образцов хлеба проводили в соответствии с методикой госкомиссии по сортоиспытанию на лабораторном хлебопекарном оборудовании. В результате определения органолептических и физико-химических показателей качества готовой продукции установлено, что добавка спирулины к пшеничной муке повышает пористость мякиша хлеба и увеличивает его объем до 546 см3, что на 18 см3 больше, чем в контрольном образце. Достаточно высокий объем 494 см3 имел образец хлеба, в рецептуру которого входит ячменная мука с добавлением спирулины. Внесение спирулины в рецептуру существенно не повлияло на показатели влажности (4346) и кислотности хлеба, которые в опытных образцах соответствуют нормативам ГОСТ 2698786 на хлеб из пшеничной муки. Самую высокую общую оценку 4,2 балла по пятибалльной шкале получил образец хлеба с добавлением к пшеничной муке порошка спирулины. The effect of the addition of spirulina microalgae in the formulation of bread from wheat flour enriched with wholemeal flour from barley grain varieties Granal 32 and Nudum 95 and triticale varieties Tsekad 90, on the indicators of its quality has been studied. Spirulina ground to a powdered state in an amount of 0,5 by weight of flour was introduced when kneading the dough into the formulations of three samples: wheat bread, bread Granal and Yasen , the formulations of which were developed earlier. Bread from wheat flour of the highest grade without additives was as a control sample. Laboratory baking of bread samples was carried out in accordance with the methodology of the state Commission for variety testing on laboratory baking equipment. As a result of the determination of organoleptic and physico-chemical indicators of the quality of the finished product, it was found that the addition of spirulina to wheat flour increases the porosity of the bread crumb and increases its volume to 546 cm3, which is 18 cm3 more than in the control sample. A sample of bread, the formulation of which includes barley flour with the addition of spirulina, had a sufficiently high volume 494 cm3. Adding spirulina to the formulation did not significantly affect the moisture content (4346) and acidity of bread, which in the prototypes correspond to the standards of GOST 2698786 for bread made of wheat flour. A sample of bread with spirulina powder added to wheat flour had the highest overall score 4б2 points on a five-point scale.


2006 ◽  
Vol 89 (4) ◽  
pp. 1012-1020 ◽  
Author(s):  
Joerg Stroka ◽  
Michelle Derbyshire ◽  
Carsten Mischke ◽  
Massimo Ambrosio ◽  
Katy Kroeger ◽  
...  

Abstract An interlaboratory study was conducted for the determination of deoxynivalenol in baby food and animal feed by high-performance liquid chromatography with UV detection. The study included 14 participants representing a cross section of industry, official food control, and research facilities. Mean recoveries reported ranged from 89% (at 120 g/kg) to 85% (at 240 g/kg) for baby food and from 100% (at 200 g/kg) to 93% (at 400 g/kg) for animal feed. On the basis of the results for spiked samples (blind duplicates at 2 levels), as well as those for naturally contaminated samples (blind duplicates at 3 levels), the relative standard deviation for repeatability (RSDr) in analyses of baby food ranged from 6.4 to 14.0% and in analyses of animal feed, from 6.1 to 16.5%. The relative standard deviation for reproducibility (RSDR) in analyses of baby food ranged from 9.4 to 19.5% and in analyses of animal feed, from 10.5 to 25.2%. The HorRat values ranged from 0.4 to 1.0 and from 0.7 to 1.3, for baby food and animal feed, respectively. The method showed acceptable performance for within-laboratory and between-laboratory precision for each matrix, as required by European legislation.


2013 ◽  
Vol 1 (04) ◽  
pp. 148-151 ◽  
Author(s):  
P. N. Rajarajan ◽  
K. M. Rajasekaran ◽  
N. K. Asha Devi

Aflatoxin is a naturally occurring Mycotoxin produced by Aspergillus flavus and Aspergillus parasiticus. Aspergillus flavus is common and widespread in nature and is most often found when certain grains are grown under stressful conditions such as draught. The mold occurs in soil, decaying vegetation, hay and grains undergoing microbiological deterioration and invades all types of organic substrates whenever and wherever the conditions are favourable for its growth. Favourable conditions include high moisture content and high temperature.The aflatoxin group is comprised of aflatoxin B1,B2,G1 and G2. In addition , aflatoxin M1 (AFM1), a hydroxylated metabolite of AFB1, is excreted in the milk of dairy cows consuming an AFB1-contaminated ration. Aflatoxin B1 a prototype of the aflatoxins, is widely recognized as the most potent hepato carcinogenic compound and along with other certain members of the group, possess additional toxic properties including mutagenicity, tetrogenicity, acute cellular toxicity and it suppresses the immune system. Aflatoxin contamination of food and feed has gained global significance as a result of its deleterious effects on human as well as animal health. The marketability of food products is adversely affected by aflatoxin contamination.


Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 121
Author(s):  
Michail D. Kaminiaris ◽  
Sophie Mavrikou ◽  
Maria Georgiadou ◽  
Georgia Paivana ◽  
Dimitrios I. Tsitsigiannis ◽  
...  

Aflatoxins are highly toxic fungal secondary metabolites that often contaminate food and feed commodities. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing monoclonal AFB1 antibodies onto a screen-printed gold electrode that was modified with carbo-methyldextran by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide cross-linking. An electrochemical interfacial modelling of biomolecular recognition was suggested and reasonably interpreted. Impedance technology was employed for the quantitative determination of AFB1. The limit of detection concentration of AFB1 for standard solutions and spiked pistachio samples was 0.5 ng/mL and 1 ng/mL, respectively. The immunosensor was able to successfully determine AFB1 concentrations in the range of 4.56–50.86 ng/mL in unknown pistachio samples. Comparative chromatographic analysis revealed that AFB1 concentrations that were higher than 345 ng/mL were not within the immunosensor’s upper limits of detection. Selectivity studies against Ochratoxin A and Aflatoxin M1 demonstrated that the proposed AFB1 immunosensor was able to differentiate between these other fungal mycotoxins. The novel electrochemical immunosensor approach has the potential for rapid sample screening in a portable, disposable format, thus contributing to the requirement for effective prevention and the control of aflatoxin B1 in pistachios.


2014 ◽  
Vol 77 (10) ◽  
pp. 1760-1767 ◽  
Author(s):  
FATEN KACHOURI ◽  
HAMIDA KSONTINI ◽  
MOKTAR HAMDI

Olives can be contaminated with a wide variety of molds (Aspergillus and/or Penicillium) that can be occurring naturally on fresh and processed olives and could support mycotoxin production. The aim of this work was to investigate aflatoxin B1 (AFB1) production by fungi and its bioaccumulation in olives during storage and to study the impact of the application of Lactobacillus plantarum on the inhibition of mold development and production of AFB1. Two different treatments were applied: (i) olives with natural microflora and (ii) olives inoculated with Aspergillus flavus after elimination of natural microflora. AFB1 has been extracted from olives and quantitated by high-performance liquid chromatography using a fluorescence detector. Results showed the absence of this metabolite in the olives for the season 2008 to 2009. In 2009 to 2010, AFB1 was detected at the level of 11 μg/kg. The application of L. plantarum during the storage of olives favors the reduction of the level of AFB1 to 5.9 μg/kg correlated with a decrease in the amount of molds (86.3%). The images obtained by environmental scanning electron microscopy showed that L. plantarum was able to adhere to the olive surface and probably produce a biofilm that inhibits the multiplication of yeast and fungi by oxygen competition. Results showed an increase of antioxidant activity and amount of total phenolic compounds of olives, respectively, by 24 and 8.6%. In many olives contaminated with A. flavus, AFB1 was present at an initial level of 5.15 μg/kg and increased to 6.55 μg/kg after 8 days of storage. The biological detoxification of AFB1 in olives by L. plantarum is confirmed by the reduction of the level of AFB1 to 2.12 μg/kg on day 0 and its absence after 4 days of storage.


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