scholarly journals FTIR-based fingerprinting combined with chemometrics for discrimination of Sonchus arvensis leaves extracts of various extracting solvents and the correlation with its antioxidant activity

2021 ◽  
pp. 132-140
Author(s):  
Mohamad Rafi ◽  
Winda Rismayani ◽  
Rita Merisa Sugiarti ◽  
Utami Dyah Syafitri ◽  
Wulan Tri Wahyuni ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Biological Activities ◽  
Principal Component ◽  
Partial Least Square ◽  
Pls Regression ◽  
Sample Extract ◽  
Dpph Method ◽  
Sonchus Arvensis ◽  
Extracting Solvent

Sonchus arvensis, the local name for tempuyung, is acknowledged to have many biological activities, including the antioxidant activity. This study aimed to cluster the leaves extracts based on the extracting solvent and to determine the functional groups significantly contributing to the antioxidant activity. From the water, 10%, 30%, 50%, 70% ethanol, and absolute ethanol extracts, we analyzed the total phenolics content (Folin-Ciocalteu method), antioxidant activity (DPPH method), and the FTIR spectra. The 70% ethanol extract exhibits the highest total phenolics and the highest antioxidant activity. The extracts were grouped based on the extracting solvent using the principal component analysis (PCA) with 95% total variance from its principal component 1 and 2. The partial least square (PLS) regression was employed for finding a functional group from the antioxidant constituents present in the sample extract. We predicted by PLS regression that the –OH and the C-O groups are attributed to the phenolics that give a significant contribution to the antioxidant activity of the S. arvensis leaves.

FTIR-based Fingerprinting and Chemometrics for Rapid Investigation of Antioxidant Activity from Syzygium polyanthum Extracts

2020 ◽  
Vol 21 (1) ◽  
pp. 128
Author(s):  
Eti Rohaeti ◽  
Fadila Karunina ◽  
Mohamad Rafi
Keyword(s):  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Functional Groups ◽  
Total Phenolics ◽  
Principal Component ◽  
Ethanolic Extract ◽  
Ftir Spectra ◽  
Partial Least Square ◽  
Least Square ◽  
Total Phenolic

Syzygium polyanthum, known as salam in Indonesia, which is rich in phenolics compounds with high antioxidant activity. In this study, we performed the determination of total phenolics and characterization of the antioxidant activity of S. polyanthum leaves extracts using the FTIR-based fingerprinting technique. The extracts of S. polyanthum in water, ethanol, and ethanol-water (30, 50, and 70%) were measured for their antioxidant activity, total phenolics, and FTIR spectra. The antioxidant activity of S. polyanthum extract with different solvent extraction showed the highest antioxidant activity and total phenolic content is 70% and 50% ethanolic extract, respectively. The FTIR spectrum of each extract showed identical FTIR spectra patterns. According to their different solvent extraction, S. polyanthum extract could be grouped based on FTIR spectra using principal component analysis. Correlation between the functional group in the FTIR spectra with IC50 from S. polyanthum extract was analyzed using partial least square (PLS). The PLS analysis results showed that O–H, C–H sp3, C=O, C=C, C-O, and C–H aromatic are the main functional groups contributed to the antioxidant activity of S. polyanthum extract. FTIR-based fingerprinting combined with chemometrics could be used to classify different extracts of S. polyanthum and predicted functional groups having a significant contribution to antioxidant activity.

scholarly journals Evaluation of phenolic compounds and antioxidant activity in some edible flowers

2020 ◽  
Vol 5 (1) ◽  
pp. 857-870
Author(s):  
Fernando Gonçalves ◽  
João Carlos Gonçalves ◽  
Ana Cristina Ferrão ◽  
Paula Correia ◽  
Raquel P. F. Guiné
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Total Phenolic ◽  
Potential Health ◽  
Phenolic Contents ◽  
Spectrophotometric Methods ◽  
Potential Source ◽  
Successive Extraction ◽  
Dpph Method ◽  
Total Anthocyanins

AbstractRecently, edible flowers (EF) have aroused increased interest because of their aesthetic properties as well as potential health benefits related to the occurrence of some bioactive compounds. Therefore, the aim of this work was to evaluate the total phenolics, anthocyanins, flavonoids, and antioxidant activity (AOA) (following DPPH and ABTS methods) in eleven EF. The samples were subjected to three successive extraction steps using methanol, and these extracts were then analysed for the aforementioned properties using spectrophotometric methods. The obtained extracts were used for the quantification of phenolic composition and AOA. The results indicated that, among the flowers analysed in this study, red rose, pink rose, and red carnation possessed the highest total phenolic contents (27.53, 23.30, and 18.17 mg g−1 gallic acid equivalents, respectively), total anthocyanins (3.07, 1.97, and 4.47 mg g−1 catechin equivalents [CE], respectively), and AOA (12.07, 15.77, and 12.93 mg g−1 TE, respectively, as given by the DPPH method or 8.23, 9.27 and 8.00 mg g−1 TE, respectively, as given by the ABTS method). The flowers with highest flavonoids contents were red carnation, Mexican marigold, and pink rose (17.50, 16.90, and 16.57 mg g−1 CE, respectively). Cluster analysis grouped the analysed flowers into two groups, those richest in phenolics with AOA and those not so rich. Finally, some important correlations were observed between the total phenolics and the AOA. In conclusion, these flowers could represent a potential source of natural compounds with antioxidant capacity.

scholarly journals Influence of Cooking Conditions on Nutritional Properties and Sensory Characteristics Interpreted by E-Senses: Case-Study on Selected Vegetables

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 607
Author(s):  
Susanna Buratti ◽  
Carola Cappa ◽  
Simona Benedetti ◽  
Gabriella Giovanelli
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Nutritional Quality ◽  
Total Phenolics ◽  
Principal Component ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Cooking Methods ◽  
Nutritional Properties ◽  
Cooking Method

This study investigates the effects of three cooking methods (boiling, steaming and microwaving) on the nutritional and physical properties of cauliflowers, carrots and sweet potatoes; e-senses were applied to interpret the sensory characteristics according to physico-chemical aspects. The nutritional quality was evaluated by determining the concentrations of key components and the antioxidant activity; e-sense data, combined with texture parameters, were processed by a principal component analysis. The cooking method and time significantly influenced the quality of the three products. Boiling, which detrimentally affected ascorbic acid, total phenolic concentration and antioxidant activity, enhanced carotene accessibility. Steaming produced losses in ascorbic acid, increasing total phenolics and carotenoids. Microwaving resulted in minor changes in ascorbic acid concentrations, preserved carotenoids and increased total phenolics. The nutritional quality was better preserved or enhanced using shorter cooking times. The elaboration of the data collected by the e-senses showed a clear evolution according to the cooking method and time. The results helped to determine the cooking method that best preserves the nutritional properties of the vegetables, highlighting the applicability of rapid instrumental methods to interpret the evolution of sensory characteristics.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SENYAWA PIRAZOLIN 3-(2- METOKSI-FENIL)-5-NAFTALEN-1-IL-4,5-DIHIDRO-1H-PIRAZOL

2018 ◽  
Vol 8 (2) ◽  
pp. 53-58
Author(s):  
Nurlaili ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Microwave Irradiation ◽  
Biological Activities ◽  
Spectroscopy Data ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Inflammatory

Pyrazoline compounds are reported to have many useful biological activities such as antimicrobials, antihyperglycemia, antioxidants, anti-inflammatory, anticancer and anticonvulsants. The pyrazoline analogues of 3- (2-methoxy-phenyl) -5-naphthalene-1-yl-4,5-dihydro-1H-pyrazol were synthesized usingglacial acetic acid as catalyst and assisted by microwave irradiation. The structures of the compounds were obtainedby UV, IR and HRMS spectroscopy data. Antioxidant activity test using DPPH method showed that pyrazoline compound had IC50 value of 27.41 μg/mL.

scholarly journals Evaluation of the antioxidant activity of some types of red and white wines

2014 ◽  
Vol 25 (2) ◽  
pp. 65-70 ◽  
Author(s):  
Ana Leahu ◽  
Sonia Amariei ◽  
Cristina Damian ◽  
Mircea Oroian ◽  
Sorina Ropciuc
Keyword(s):  
Antioxidant Activity ◽  
Multivariate Data Analysis ◽  
Red Wine ◽  
Total Phenolics ◽  
Principal Component ◽  
White Wine ◽  
Total Phenols ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
White Wines

Abstract Samples of commercially available Romanian wines were analyzed in order to determine total phenols content and the antioxidant activity. The content of total phenolics in the extracts was determined according to the Folin-Ciocalteu method and calculated as gallic acid equivalents (mg GAE/100g). Antiradical activities of the extracts were evaluated by a micro assay using 1, 1¢-diphenyl-2-picrylhydrazyl spectrophotometric method. Wine characteristics measurements were examined by multivariate data analysis, using principal component analysis (PCA). Total polyphenol content was correlated to the antioxidant activity of the studied wine samples. The values of the inhibition power of free radical, PI%, are ranging between 1.68 for white wine and 0.95 for red wine (“Bull blood” bottled by Tohani winery, Prahova, Romania).

Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.

10.5219/1360 ◽  
2020 ◽  
Vol 14 ◽  
pp. 393-401
Author(s):  
Yulia Vinogradova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Eva Ivanišová ◽  
Ján Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Biologically Active ◽  
Polyphenol Compounds ◽  
Dpph Method ◽  
Ethanol Extracts

Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.

scholarly journals DETERMINATION OF BIOLOGICAL ACTIVITIES OF THREE MARINE ALGAE COLLECTED FROM VISAKHAPATNAM COAST

2017 ◽  
Vol 10 (12) ◽  
pp. 274 ◽  
Author(s):  
Satya Lakshmi S
Keyword(s):  
Antioxidant Activity ◽  
Marine Algae ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Dpph Method ◽  
Anti Inflammatory

  Objective: Determination of biological activities of marine algae collected from the Visakhapatnam coastal region.Methods: Antibacterial activity of algal extracts determined by the well diffusion method, antioxidant activity was determined by reducing power (RP) method and 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method finally anti-inflammatory activity was determined by human red blood cell stabilization method and egg albumin method.Results: Methanolic extracts of three algae have potential inhibition activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Green alga Enteromorpha compressa has better antioxidant activity compared to the Gracilaria arcuata and Ulva fasciata when tested in RP and DPPH method. U. fasciata found good anti-inflammatory activity among the selected three algae.Conclusion: The three tested algae exhibited significant antibacterial and antioxidant activity compared to anti-inflammatory activity. These bioactive compounds containing macroalgae may find their commercial potential in medicine, food, and cosmetic industry

scholarly journals Comparison of the Antioxidant Activities and Phenolic Content of Five Lonicera Flowers by HPLC-DAD/MS-DPPH and Chemometrics

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Rong-rong Zhou ◽  
Xue-hui Liu ◽  
Lin Chen ◽  
Jian-hua Huang ◽  
Xue-juan Liang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Potential Health ◽  
Caffeoylquinic Acid ◽  
Acid Methyl ◽  
Dpph Method ◽  
Comprehensive Development

The Lonicera plants (family Caprifoliaceae) with strong antioxidant activity are used as potential health-supporting phytochemicals. Studying the detailed relationships between bioactive compounds and their antioxidant activity is important for further comprehensive development and application of them. In this paper, the antioxidant capacities and compositions of five species of Lonicera flowers were investigated by using the online HPLC-DAD/MS-DPPH method. Results indicated that the samples contained higher amounts of phenols had better antioxidant activity. Furthermore, principal component analysis and linear regression were further used to analyze the correlations between antioxidant capacity and compounds and find the compounds having higher contribution to antioxidant activity. 5-O-Caffeoylquinic acid, 4-O-caffeoylshikimic acid, methyl-5-O-caffeoylquinate, 1,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid were screened as stronger antioxidant candidates. In this study, HPLC-DAD/MS and antioxidant activity methods were combined together to analyze the compounds’ information and activity assays of Lonicera, which might provide more evidence for its quality control.

scholarly journals Aktivitas Antioksidan Protein Hidrolisat Dari Kasein Susu Kambing Etawa Hasil Hidrolisis Bromelin Dari Daun Nanas Madu

2020 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Puji Lestari
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Goat Milk ◽  
Degree Of Hydrolysis ◽  
Protein Fragments ◽  
Protein Hydrolyzate ◽  
Hydrolyzed Protein ◽  
Casein Hydrolyzate ◽  
Dpph Method ◽  
Hemolysis Test

Hydrolyzed proteins are fragments of protein fragments that have certain biological activities. Some of the functions of hydrolyzed proteins that are known are as antibacterial, antioxidant, antihypertensive, antithrombotic, anti-inflammatory, opioid, and immunomodulatory. Protein from Etawa goat's milk is one source of protein hydrolyzate. In this research, the antioxidant activity of protein hydrolyzate from casein Etawa goat milk was tested. Casein is hydrolyzed with bromelain from honey pineapple leaves. The research steps are: bromelain extraction, casein hydrolysis of Etawa goat milk, antioxidant activity fraction of hydrolyzed protein fractions by DPPH method, and hemolysis test. The results of the study were: (1) the highest degree of hydrolysis in casein hydrolyzate was achieved at 60 minutes incubation time, (2) casein hydrolyzate which had the highest antioxidant activity was incubated for 30 minutes, (3) based on the AAI value, the casein hydrolyzed activity included into the classification of weak antioxidants because AAI <0.5, (4) the results of the hemolysis test show that casein hydrolyzate does not cause hemolysis of red blood cells

scholarly journals Free Volatile Compounds of Veronica austriaca ssp. jacquinii (Baumg.) Eb. Fisch. and Their Biological Activity

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2529
Author(s):  
Marija Nazlić ◽  
Željana Fredotović ◽  
Elma Vuko ◽  
Nenad Vuletić ◽  
Ivica Ljubenkov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Volatile Compounds ◽  
Glandular Trichomes ◽  
Principal Component ◽  
Stalk Cell ◽  
Total Phenolic ◽  
Dpph Method ◽  
Hexahydrofarnesyl Acetone ◽  
Different Types

The composition of free volatile compounds of essential oils (EO) and hydrosols (Hy) from four different localities of the species Veronica austriaca ssp. jacquinii (Baumg.) Eb. Fisch. were analyzed by gas chromatography coupled with mass spectrometry. In the EOs, the most abundant compounds identified were hexahydrofarnesyl acetone (23.34–52.56%), hexadecanoic acid (palmitic acid, 26.71–58.91%) and octadecanol acetate (0–6.24%). The hydrosols were characterized by high abundance of methyl eugenol (23.35–57.93%), trans-p-mentha-1(7),8-dien-2-ol (5.24–7.69%) and thymol (3.48–9.45%). Glandular trichomes were analyzed using SEM (Scanning Electron Microscopy), as they are the sites of synthesis of free volatile compounds. We have detected glandular trichomes, consisting of a one stalk cell and two elliptically shaped head cells, and non-glandular (unbranched, bi-cellular to multicellular) trichomes on stems, leaves and the sepals. Data for volatile compounds from EOs and hydrosols were analyzed using Principal Component Analyses (PCA) to demonstrate variations in the composition of the volatile compounds identified. Isolated samples of EO and hydrosols were analyzed for their antioxidant activity using two methods, DPPH (2,2-diphenyl-1-picrylhydrazyl) and ORAC (Oxygen Radical Absorbance Capacity). The essential oils showed higher antioxidant activity than the hydrosols in ORAC method, but lower activity by the DPPH method. The isolates were also tested for their antiproliferative activity on different types of cancer cells and also on two lines of healthy cells, and the results showed that the extracts were not toxic to the cell lines tested. Total polyphenols, total tannins, total flavonoids and total phenolic acids were also analyzed and determined spectrophotometrically. The free volatile compounds of Veronica austriaca ssp. jacquinii can be considered as a safe natural product.

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