scholarly journals Preparation, Characterization, and In Vitro Hemocompatibility of Glutaraldehyde-Crosslinked Chitosan/Carboxymethylcellulose as Hemodialysis Membrane

2021 ◽  
Vol 21 (5) ◽  
pp. 1120
Author(s):  
Khabibi Khabibi ◽  
Dwi Siswanta ◽  
Mudasir Mudasir
Keyword(s):  
Mechanical Strength ◽  
Protein Adsorption ◽  
Partial Thromboplastin Time ◽  
Carboxymethyl Cellulose ◽  
Phase Inversion ◽  
Inversion Method ◽  
Crosslinked Chitosan ◽  
Phase Inversion Method ◽  
Hemodialysis Membrane

This study aims to examine the manufacture, characterization, and in vitro hemocompatibility of glutaraldehyde-crosslinked chitosan/carboxymethyl cellulose (CS/CMC-GA) as a hemodialysis membrane. The CS/CMC-GA membrane was prepared using the phase inversion method with 1.5% CS and 0.1% CMC. The chitosan was crosslinked with glutaraldehyde in various monomers ratios, and the membranes formed were characterized by FTIR, SEM, and TGA. Furthermore, the hydrophilicity, swelling, porosity, mechanical strength, and dialysis performance of the membranes against urea and creatinine were systematically examined, and their in-vitro hemocompatibility tests were also conducted. The results showed that the CS/CMC-GA membranes have higher hydrophilicity, swelling, porosity, mechanical strength, and better dialysis performance against urea and creatinine than chitosan without modification. In addition, the hemocompatibility test indicated that the CS/CMC-GA membranes have lower values of protein adsorption, thrombocyte attachment, hemolysis ratio, and partial thromboplastin time (PTT) than that of pristine chitosan. Based on these results, the CC/CMC-GA membranes have better hemocompatibility and the potential to be used as hemodialysis membranes.

scholarly journals Pengaruh Penambahan Epiklorohidrin Terhadap Sifat Mekanik dan Daya Serap Film Khitosan Sebagai Adsorben

2017 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Nisfayati Juir ◽  
Rahmi Rahmi ◽  
Marlina Marlina
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Tensile Test ◽  
Phase Inversion ◽  
Crosslinking Agent ◽  
Chitosan Film ◽  
Inversion Method ◽  
Test Results ◽  
Crosslinked Chitosan ◽  
Phase Inversion Method

Pembuatan film khitosan berikatan silang epiklorohidrin telah dilakukan dengan metode inversi fasa. Pada penelitian ini khitosan dimodifikasi dengan epiklorohidrin untuk memperbaiki sifat mekanik dan ketahanannya terhadap asam. Film khitosan hasil modifikasi diuji sifat mekaniknya dengan alat uji tarik dan daya serapnya diuji terhadap ion Cd2+. Hasil uji tarik menunjukkan bahwa dengan adanya pembentuk ikat silang epiklorohidrin  pada khitosan dapat meningkatkan tensile strength film khitosan dan optimum pada komposisi 54,128 % b/b. Hasil uji adsorpsi terhadap ion Cd2+ menunjukkan bahwa penambahan epiklorohidrin dapat meningkatkan kapasitas penyerapan ion Cd2+ sebesar 31,33 %  dibandingkan dengan film khitosan tanpa silang.  Preparation of epichlorohydrin crosslinked  chitosan film has been done by phase inversion method. In this study, chitosan was modified with epichlorohydrin by the purpose of improving its  mechanical properties and adsorption capasity. Mechanical properties of crosslinked chitosan film was examined by tensile test. Adsorption study was done for Cd2+ ions removal. Tensile test results showed  epichlorohydrin as crosslinking agent of chitosan can improve tensile strength and optimum at 54.128 % w/w of composition. The adsorption results for Cd2+ ions showed the addition of epichlorohydrin in chitosan can increase the adsorption capacity of Cd2+ ions 31,33 % compared with non-crosslinked chitosan films.

scholarly journals Bilayered Fibrin-Based Electrospun-Sprayed Scaffold Loaded with Platelet Lysate Enhances Wound Healing in a Diabetic Mouse Model

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2128
Author(s):  
Paola Losi ◽  
Tamer Al Kayal ◽  
Marianna Buscemi ◽  
Ilenia Foffa ◽  
Aida Cavallo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Phase Inversion ◽  
Diabetic Mouse ◽  
Platelet Lysate ◽  
Inversion Method ◽  
Post Surgery ◽  
Phase Inversion Method

The present study examined the effects of a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate by a combination of electrospinning and spray, phase-inversion method for wound healing. In particular, the poly(ether)urethane layer was obtained using by a spray phase-inversion method and the fibrin fibers network were loaded with platelet lysate by electrospinning. The kinetics release and the bioactivity of growth factors released from platelet lysate-scaffold were investigated by ELISA and cell proliferation test using mouse fibroblasts, respectively. The in-vitro experiments demonstrated that a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate provides a sustained release of bioactive platelet-derived growth factors. The effect of a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate on wound healing in diabetic mouse (db/db) was also investigated. The application of the scaffold on full-thickness skin wounds significantly accelerated wound closure at day 14 post-surgery when compared to scaffold without platelet lysates or commercially available polyurethane film, and at the same level of growth factor-loaded scaffold. Histological analysis demonstrated an increased re-epithelialization and collagen deposition in platelet lysate and growth factor loaded scaffolds. The ability of bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate to promote in-vivo wound healing suggests its usefulness in clinical treatment of diabetic ulcers.

A straight, open and macro-porous fuel electrode-supported protonic ceramic electrochemical cell

2021 ◽  
Author(s):  
Yuxin Pan ◽  
Kai Pei ◽  
Yucun Zhou ◽  
Tong Liu ◽  
Meilin Liu ◽  
...  
Keyword(s):  
Phase Inversion ◽  
Electrochemical Cell ◽  
Inversion Method ◽  
Phase Inversion Method

A straight, open and macro-porous Ni–BaZr0.1Ce0.7Y0.1Yb0.1O3 fuel electrode-supported protonic ceramic electrochemical cell has been fabricated by a modified phase-inversion method.

A novel PVFM based membranes prepared via phase inversion method and its application in GPE

Polymer ◽  
2013 ◽  
Vol 54 (18) ◽  
pp. 4807-4813 ◽  
Author(s):  
Yan Ren ◽  
Fang Lian ◽  
Yan Wen ◽  
Hong-Yan Guan
Keyword(s):  
Phase Inversion ◽  
Inversion Method ◽  
Phase Inversion Method

scholarly journals Formulation, characterization and stability of o/w nanoemulsion containing rice bran oil prepared by emulsion phase inversion

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1025-1029
Author(s):  
Y.P. Sari ◽  
S. Raharjo ◽  
U. Santoso ◽  
Supriyadi
Keyword(s):  
Rice Bran ◽  
Phase Inversion ◽  
Rice Bran Oil ◽  
Coconut Oil ◽  
Tween 80 ◽  
Polydispersity Index ◽  
Inversion Method ◽  
Emulsion Phase ◽  
Naturally Occurring ◽  
Phase Inversion Method

Rice bran oil (RBO) contains naturally occurring antioxidants such as carotenoids, tocopherol, and γ-oryzanol. The aim of this research was to formulate and evaluate the characteristics of nanoemulsion which was prepared using RBO containing naturally occurring antioxidants. The RBO-in-water nanoemulsion was prepared by the emulsion phase inversion method. The oil phase of the nanoemulsion was prepared by either virgin coconut oil (VCO) or palm oil (PO) combined with RBO with the ratio of 5:5; 4:6, 3:7, 2:8 and 0:10. Tween 80 was used as a surfactant. The surfactant to oil ratios was predetermined at 2.5:1.0 and 3.0:1.0. The aqueous phase (80% w/w) was titrated into an organic phase that consisted of Tween 80 and oil phase (approximately 20% w/w). Droplet size, zeta-potential and polydispersity index of the nanoemulsion were used as the main parameters. The results showed that the smallest droplet (<100 nm) of the nanoemulsion was obtained when the ratio of VCO: RBO at 3:7 and the ratio of PO: RBO at 4:6 with the surfactant to oil ratio (SOR) was 2.5. Nanoemulsion with a relatively small polydispersity index of 0.3 was achieved when the ratio of PO: RBO was 3:7 and SOR at 3. All of the freshly prepared RBO containing nanoemulsion have good stability with zetapotential values of < -30 mV. Nanoemulsions were stable against centrifugation at 2300 rpm for 15 mins, but they were not stable against heating at 105°C for 5 hrs. The RBO-inwater nanoemulsion could be successfully prepared by phase inversion method, by combining RBO with either VCO or PO at different ratios.

Sign in / Sign up

Export Citation Format

Share Document