scholarly journals Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using p-Amino-N,N-dimethylaniline as a Derivatizing Agent

2020 ◽  
Vol 21 (1) ◽  
pp. 192
Author(s):  
Bahaa Malik Altahir ◽  
Omar Abdulazeez ◽  
Sarmad Bahjat Dikran ◽  
Keith Edward Taylor
Keyword(s):  
Oxidative Coupling ◽  
Extraction Procedure ◽  
Personal Care Products ◽  
Calibration Plot ◽  
Personal Care ◽  
Derivatizing Agent ◽  
Colored Product ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Two simple methods for the determination of eugenol were developed. The first depends on the oxidative coupling of eugenol with p-amino-N,N-dimethylaniline (PADA) in the presence of K3[Fe(CN)6]. A linear regression calibration plot for eugenol was constructed at 600 nm, within a concentration range of 0.25-2.50 μg.mL–1 and a correlation coefficient (r) value of 0.9988. The limits of detection (LOD) and quantitation (LOQ) were 0.086 and 0.284 μg.mL–1, respectively. The second method is based on the dispersive liquid-liquid microextraction of the derivatized oxidative coupling product of eugenol with PADA. Under the optimized extraction procedure, the extracted colored product was determined spectrophotometrically at 618 nm. A linear plot within a concentration range of 0.05–1.65 μg.mL–1 (r = 0.9997) was constructed. The LOD and LOQ were 0.053 and 0.177 μg.mL–1, respectively. Both methods were tested for the analysis of eugenol in commercial personal-care products, and the results confirmed that the procedures are accurate, precise, and reproducible (RSD < 1%).

Dispersive Liquid–Liquid Microextraction for HPLC-UV Determination of PAHs in Milk

2016 ◽  
Vol 99 (2) ◽  
pp. 527-533 ◽  
Author(s):  
Mansour Mahmoudpour ◽  
Javad Mohtadinia ◽  
Masood Ansarin ◽  
Mahboob Nemati
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Milk Sample ◽  
Aromatic Hydrocarbons ◽  
Extraction Procedure ◽  
Correlation Coefficients ◽  
Extraction Solvent ◽  
Polycyclic Aromatic ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract A simple and rapid analytical method for the extraction and quantification of four polycyclic aromatic hydrocarbons in milk sample has been developed using dispersive liquid–liquid microextraction followed by the use of HPLC. Benzo(a)pyrene, benzo(a)anthracene, and benzo(b)fluoranthene were used as model compounds; the milk sample was spiked with these compounds to assess the extraction procedure. Experimental parameters that influence the extraction efficiency, including the nature and volume of the disperser and extraction solvents, pH, and the volume of milk sample, were optimized. Under the optimum extraction conditions (extraction solvent: chloroform, 200 μL; dispersive solvent: acetonitrile, 700 μL; and extraction time 5 s or less), the performance of the proposed method was evaluated. The chromatographic peak area was linear with concentrations in the range of 0.2–10 ng/mL−1 and with correlation coefficients ranging from 0.9968 to 0.9985. The LODs, based on a S/N ratio of 3, ranged from 0.06 to 0.18 ng/mL−1. The RSDs varied from 3.68 to 9.7% (n = 3). The recoveries of these compounds were from 88.38 to 100%. The performance of the present method was evaluated for the determination of polycyclic aromatic hydrocarbons in various types of milk samples.

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