scholarly journals Wild Boar-Specific PCR Assay and Sequence Analysis Based on Mitochondrial Cytochrome-B Gene for Halal Authentication Studies

2020 ◽  
Vol 20 (2) ◽  
pp. 483
Author(s):  
Ganea Qorry Aina ◽  
Abdul Rohman ◽  
Yuny Erwanto
Keyword(s):  
Sequence Analysis ◽  
Wild Boar ◽  
Cytochrome B ◽  
Coefficient Of Determination ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Mt Dna ◽  
Mitochondrial Cytochrome B ◽  
Amplicon Size ◽  
Halal Authentication

Wild boar meat (WBM) is non-halal meat widely abused in Indonesia. The most common case is mixing beef with WBM either in raw or processed foods. Therefore, it is necessary to develop a detection method of WBM contamination. The objective of this study was to employ polymerase chain reaction (PCR) and sequence analysis using species specific primer (SSP) targeting on wild boar mitochondrial cytochrome-b (CYTBWB2-wb) gene for the identification of WBM in a meatball. The specificity of primer was tested, and the amplicon size was confirmed with conventional PCR and agarose electrophoresis. The base sequences were analyzed using GeneStudio software and subjected to BLAST using NCBI. CYTBWB2-wb primer was also used to test the reference meatballs made from beef and WBM using real-time PCR. The result showed that CYTBWB2-wb amplified wild boar Cyt-B mt-DNA gene specifically. The amplicon size was 194 base pair (bp) with a similarity of 93–98% toward gen Cyt-B mt-DNA of several wild boar types. The primer is able to detect WBM on the reference meatballs up to 0.1% wt/wt with efficiency value of 108.0% and coefficient of determination (R2) of 0.970. The CYTBWB2-wb primer proved to be specific and could be used as a standard method to identify the presence of WBM contamination in meatball products for halal authentication studies.

Mitochondrial Cytochrome b DNA Sequence Variations: An Approach to Fish Species Identification in Processed Fish Products

2005 ◽  
Vol 68 (2) ◽  
pp. 421-425 ◽  
Author(s):  
TIZIANA PEPE ◽  
MICHELE TROTTA ◽  
ISOLINA DI MARCO ◽  
PAOLA CENNAMO ◽  
ANIELLO ANASTASIO ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Cytochrome B ◽  
Fish Species ◽  
Mitochondrial Cytochrome ◽  
Fish Products ◽  
Community Regulation ◽  
Sequence Variations ◽  
Fish Species Identification ◽  
Mitochondrial Cytochrome B ◽  
Pcr Products

The identification of fish species in food products is problematic because morphological features of the fish are partially or completely lost during processing. It is important to determine fish origin because of the increasing international seafood trade and because European Community Regulation 104/2000 requires that the products be labeled correctly. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. This method allowed the identification of fish species in all samples. Fish in all of the examined products belonged to these two families, with the exception of one sample of smoked baccalà (salt cod), which was not included in the Gadidae cluster.

scholarly journals Kajian Penanda Genetik Gen Sitokrom b DNA Mitokondria Ikan Lais dari Sungai Kampar Riau

2018 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Roza Elvyra ◽  
Dedy Duryadi Solihin
Keyword(s):  
Species Diversity ◽  
Molecular Marker ◽  
Phylogenetic Relationship ◽  
Cytochrome B ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Phylogenetic Marker ◽  
Mitochondrial Cytochrome B ◽  
Cyt B Gene

The mitochondrial cytochrome b (cyt-b) gene as a phylogenetic marker of lais fish Kryptopterus schilbeides from Kampar River in Riau has been studied. This is a prelimininary research on the utility of cyt-b gene as a molecular marker to obtain species diversity and phylogenetic relationship among Kryptopterus fishes from Kampar River. The primers of L14841 and H15149 were used to amplify the cyt-b gene. The results showed that K. schilbeides has isoleusine at site-81 and metionine at site-114; K. schilbeides from Kampar River and K. schilbeides from GenBank form a phylogeny cluster at 45% value.

scholarly journals Partial sequence analysis of mitochondrial cytochrome B gene of Labeo calbasu of Bangladesh

2019 ◽  
Vol 5 (1) ◽  
pp. 25-30
Author(s):  
RA Begum ◽  
MT Alam ◽  
H Jahan ◽  
MS Alam
Keyword(s):  
Genetic Diversity ◽  
Cytochrome B ◽  
Tissue Sample ◽  
Sequence Similarity ◽  
Cytochrome B Gene ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Multiple Sequence ◽  
Mitochondrial Cytochrome B ◽  
Cyt B Gene

Labeo calbasu (Family Cyprinidae) was studied at DNA level to know genetic diversity within and between species. The mitochondrial cytochrome b (cyt-b) gene of L. calbasu was sequenced and compared to the corresponding sequences of other Labeo species. DNA was isolated from the tissue sample of L. calbasu using phenol: chloroform extraction method. Forward and reverse primers were designed to amplify the target region of cytochrome b gene. A standard PCR protocol was used for the amplification of the desired region. Then, the forward and reverse sequences obtained were aligned and edited to finalize a length of 510 nucleotides which was submitted to NCBI genbank database. Nucleotide BLAST of this sequence at NCBI resulted 100% sequence similarity with L. calbasu sequence of the same region of cyt-b gene. Multiple sequence alignment of the sequence with seven more Labeo species sequences revealed 120 polymorphic sites, which have been mark of diversity among the species and might be used in molecular identification of the Labeo species. A constructed phylogenetic tree has shown relationship among the Labeo species. This research demonstrated the usefulness of mitochondrial DNA-based approach in species identification. Further, the data will provide appropriate background for studying genetic diversity within-species of the Labeo species in general and of L. calbasu in particular. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(1): 25-30

Comparison of mitochondrial cytochrome b lineages and morphospecies of two avian malaria parasites of the subgenera Haemamoeba and Giovannolaia (Haemosporida: Plasmodiidae)

Zootaxa ◽  
2007 ◽  
Vol 1626 (1) ◽  
pp. 39-50 ◽  
Author(s):  
VAIDAS PALINAUSKAS ◽  
VLAD KOSAREV ◽  
ANATOLY SHAPOVAL ◽  
STAFFAN BENSCH ◽  
GEDIMINAS VALKINAS
Keyword(s):  
Cytochrome B ◽  
Avian Malaria ◽  
Passer Domesticus ◽  
Morphological Identification ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Malaria Parasites ◽  
Mitochondrial Cytochrome B ◽  
The Baltic ◽  
Avian Malaria Parasites

PCR-based methods have been increasingly used in diagnosis of parasitic diseases. Over 40 morphospecies of avian malaria parasites of the genus Plasmodium have been described. However, only nine of them have been identified on the level of their mitochondrial cytochrome b (cyt b) gene lineages, which are frequently used in molecular biology studies of avian blood haemosporidian parasites. In this study, we linked two common mitochondrial cyt b lineages P-SGS1 and P-TURDUS1 with their morphospecies. Light infections with two species of malaria parasites of the subgenera Haemamoeba and Giovannolaia were isolated from naturally infected adult Hawfinches, Coccothraustes coccothraustes Linnaeus, on the Curonian Spit in the Baltic Sea. These parasites were inoculated to naive juveniles of the Common Crossbill, Loxia curvirostra Linnaeus, and House Sparrow, Passer domesticus Linnaeus. Heavy parasitemia of Plasmodium (Haemamoeba) relictum Grassi & Feletti, 1891 (lineage P-SGS1) and Plasmodium (Giovannolaia) circumflexum Kikuth, 1931 (P-TURDUS1) developed in the subinoculated Common Crossbills and House Sparrows, respectively, which enabled the detailed illustration of all main blood stages of these parasites and the deposition of their voucher specimens. The parasites of both lineages are actively transmitted in Europe and inhabit a broad range of avian hosts. Lineages closely related to P. relictum and P. circumflexum were identified. This study contributes to establishing of combined PCR-based and morphological identification of avian malaria parasites.

scholarly journals Molecular detection of cattle and buffalo species meat origin using mitochondrial cytochrome b (Cyt b) gene

2016 ◽  
Vol 2 (2) ◽  
pp. 177-182
Author(s):  
Sirazum Munira ◽  
Fatema Tuz Jahura ◽  
Md Munir Hossain ◽  
Mohammad Shamsul Alam Bhuiyan
Keyword(s):  
Cytochrome B ◽  
Genomic Dna ◽  
Molecular Technique ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Dna Yield ◽  
Mitochondrial Cytochrome B ◽  
Meat Samples ◽  
Species Specific ◽  
Cyt B Gene

The study was conducted to adopt PCR based technique for identification of species origin from meat samples of cattle and buffalo using mitochondrial cytochrome b (Cyt b) gene fragment. A total of 42 ear tissue and meat samples were collected from different slaughterhouses and farms of Mymensingh, Bogra and Rangpur districts and stored in 96% ethanol at room temperature. Genomic DNA was extracted from all samples using GeNet Bio genomic DNA isolation kit. The average DNA yield of considered samples was found 204.57 ng/?l where the purity ranged from 1.82–1.99. Two (2) pair species-specific primers were used to amplify Cyt b gene fragments of 472 bp and 124 bp for cattle and buffalo, respectively. The PCR results revealed different species specific amplified fragments which could discriminate between cattle (472 bp) and buffalo (124 bp) species precisely from pure and mixed samples of those species. This study suggests an accurate molecular technique for identification of cattle and buffalo species meat origin and differentiates species present in adulterated meat samples. In conclusion, this DNA based technique could be utilized for prevention of malpractice in slaughterhouse and chain shops and thereby to protect consumer’s right.Asian J. Med. Biol. Res. June 2016, 2(2): 177-182

scholarly journals An analysis of polymorphism and phylogenetic tree using mitochondrial cytochrome B of wild boar in central Vietnam highlands

2012 ◽  
Vol 34 (3se) ◽  
Author(s):  
Nguyen Thi Phuong Mai ◽  
Le Ngoc Chau ◽  
Nguyen Khac Duy ◽  
Le Thanh Long ◽  
Hoang Nghia Son
Keyword(s):  
Phylogenetic Tree ◽  
Wild Boar ◽  
Cytochrome B ◽  
Mitochondrial Cytochrome ◽  
Central Vietnam ◽  
Mitochondrial Cytochrome B

Phylogenetic Affi nities of the Globodera pallida Inferred From the mtDNA cyt-b Gene Polymorphism

2014 ◽  
Vol 1 (2) ◽  
pp. 3-11
Author(s):  
L. Pylypenko ◽  
V. Blok ◽  
M. Phillips
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Variability ◽  
Cytochrome B ◽  
Software Package ◽  
Cytochrome B Gene ◽  
Mitochondrial Cytochrome ◽  
Cyt B ◽  
Sources Of Resistance ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene

The mitochondrial cytochrome b gene marker was used to investigate the genetic variability of G. pallida populations of different origins and selection on three sources of resistance. Aim. To sequence the mitochon- drial gene cyt-b and to clarify its application as a genetic marker for intraspecifi c genetic diversity study, phylogenetic analysis and nematode virulence assessment. Methods. The cysts of nematodes were used as a source for DNA extraction. Polymerase chain reaction was performed using the specifi c primers of INRAcytbL and INRAcytbR, followed by the amplifi ed product sequencing. The nucleotide sequences were processed and aligned using software PhredPhrap, CONSED and Clustal W. MEGA-4, DNADIST software package; while PHYLIP and Arlequin were used for statistical analysis. Phylogenetic trees construction and visualization were performed using the software package PHYLIP and TREEVIEW. Results. The phylogenetic analysis based on mitochondrial cytochrome b gene sequences has showed that the Ukrainian populations of G. pallida were almost identical to other populations from the Europe. Limited genetic variability was observed between G. pallida populations distributed in the Europe and Ukraine, accounting for 82.3 per cent (P < 0.05) of the genetic variability inferred from the mitochondrial cytochrome b gene polymorphisms within the populations studied. G. pallida populations selected on three sources of resistance were similar but not identical indicat- ing that changes in mtDNA haploid type frequency had taken place as a result of the selection regime, but the marker used was not yet applicable for virulence monitoring. Conclusions. The obtained data prove the hypothesis that G. pallida populations in Ukraine are the result of the continuing spread of the species within the Europe and not the consequence of additional introduction from the South America.

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