scholarly journals Effect of Salinity and Oxygen Condition on Phosphate Release from Marine Sediment Measured Using Diffusive Gradient in Thin Film (DGT) Technique

2019 ◽  
Vol 19 (3) ◽  
pp. 615
Author(s):  
Askal Maimulyanti ◽  
Budiawan Budiawan ◽  
Asep Saefumillah ◽  
Heny Suseno

The diffusive gradient in thin film (DGT) is an analytical technique to determine phosphate in the environment. This technique uses a thin film diffusive hydrogel in contact with a binding phase (ferrihydrite) to binding of phosphate. The released phosphate from marine sediment of Jakarta Bay was studied by DGT technique for effect of salinity and oxygen condition. Effect of salinity was observed by NaCl concentration from 0-35 g/L. The maximum phosphate release from sediment was occurred at the concentration of NaCl 30 g/L with incubation for 15 days with phosphate released at 113.99 μg/L, MDGT of 4.7723 μg and CDGT of 17.56 μg/L. The experiment showed the increase of MgCl2 and CaCl2 concentration reduced phosphate release from sediment. The condition of oxygen indicating the release of phosphate under anaerobic conditions is greater than the aerobic condition. The aerobic conditions with incubation for 21 days showed the release of phosphate from sediment to overlying water of 124.72 μg/L, MDGT of 2.4492 μg and CDGT of 6.4380 μg/L. Anaerobic conditions with incubation for 21 days showed phosphate release from sediment to overlying water of 208.62 μg/L, MDGT of 6.1081 μg and CDGT of 16.06 μg/L. The experiment shows that salinity and oxygen concentration influences phosphate release from marine sediment of Jakarta Bay.

2021 ◽  
Vol 14 (01) ◽  
pp. 561-568
Author(s):  
Askal Maimulyanti ◽  
Anton Restu Prihadi ◽  
Asep Saefumillah

2000 ◽  
Vol 42 (3-4) ◽  
pp. 265-272 ◽  
Author(s):  
T. Inoue ◽  
Y. Nakamura ◽  
Y. Adachi

A dynamic model, which predicts non-steady variations in the sediment oxygen demand (SOD) and phosphate release rate, has been designed. This theoretical model consists of three diffusion equations with biochemical reactions for dissolved oxygen (DO), phosphate and ferrous iron. According to this model, step changes in the DO concentration and flow velocity produce drastic changes in the SOD and phosphate release rate within 10 minutes. The vigorous response of the SOD and phosphate release rate is caused by the difference in the time scale of diffusion in the water boundary layer and that of the biochemical reactions in the sediment. Secondly, a negative phosphate transfer from water to sediment can even occur under aerobic conditions. This is caused by the decrease in phosphate concentration in the aerobic layer due to adsorption.


2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.


2018 ◽  
Vol 107 (1) ◽  
pp. 39-54
Author(s):  
Chunli Wang ◽  
Xiaoyu Yang ◽  
Jiangang He ◽  
Fangxin Wei ◽  
Zhong Zheng ◽  
...  

Abstract To explore the diffusion behavior of 75Se(IV) in Beishan granite (BsG), the influences of temperature, oxygen condition and ionic strength were investigated using the through-diffusion experimental method. The effective diffusion coefficient De of 75Se(IV) in BsG varied from 4.21×10−14 m2/s to 3.19×10−13 m2/s in our experimental conditions, increased with increasing temperature. The formation factor Ff of BsG was calculated to be nearly constant in the range of temperatures investigated, suggesting that the inner structure of BsG had no significant change in the temperature range of 20–55°C. Meanwhile, the De values of 75Se(IV) in BsG under anaerobic condition was significantly larger than that under aerobic condition, which may be attributed to the difference in the sorption characteristics and species distribution of Se and pH values. Moreover, the diffusion of 75Se(IV) was promoted with ionic strength increased from 0.01 M to 0.1 M, and then decreased at 0.5 M, mainly due to the combined effects of reduced double layers with increased ionic strength and increase of the solution viscosity at higher ionic strength.


2020 ◽  
Vol 20 (2) ◽  
pp. 395
Author(s):  
Ardina Purnama Tirta ◽  
Asep Saefumillah ◽  
Foliatini Foliatini ◽  
Herawati Herawati

The phenomenon of phosphate release in sediments into water bodies under oxic environment has been investigated using the Diffusive Gradient in Thin Film (DGT) technique. This research consists of several stages: polymer synthesis and DGT probe assembly, sediment sampling, DGT deployment in oxic conditions, and phosphate analysis from DGT adsorption results. Acrylamide polymer was successfully synthesized with a composition 15% acrylamide; N-N'-methylenebisacrylamide 0.1% and ferrihydrite as binding gels. DGT probes were assembly by placing a 16 x 3.2 cm polyacrylamide gel, binding gels and filter membranes on the DGT probes. The sediment sample was taken from the Bogor Botanical Gardens at the coordinates 6°36’00.6” S; 106°47’51.0” E. The DGT probe was placed in sediment samples for 1, 3 and 7 days in oxic conditions. After the prescribed time, the binding gel was removed and cut every 1 cm depth, then eluted using 0.25 M H2SO4 and the phosphate concentration was measured using spectrophotometry method. The results showed that the phosphate concentration tends to be higher with the increasing incubation time and depth. Maximum CDGT phosphate released on day 1, day 3 and day 7 were 1.00 µg/L at a depth of 14 cm, 6.61 µg/L at a depth of 14 cm, and 20.92 µg/L at a depth of 11 cm respectively. This ensures that the phosphate in water bodies comes from biogeochemical processes that occur in sediments and is successfully measured through DGT techniques.


1962 ◽  
Vol 202 (5) ◽  
pp. 981-983 ◽  
Author(s):  
John M. Ginski ◽  
John F. Thomson ◽  
Akira Omachi

P32 release from dog erythrocytes previously incubated with radioactive inorganic phosphate was investigated. Under both aerobic and anaerobic conditions, apparent activation energies of similar magnitude, 25,000 cals/mole, were obtained. However, P32 release was lower in the absence of oxygen and greater in the presence of iodoacetate. These results suggest that the cellular pool from which P32 is released is accessible to glycolytic enzymes and that the rate of P32 release is related in some manner to the orthophosphate concentration in the precursor pool.


2013 ◽  
Vol 9 (1-2) ◽  
pp. 9-15 ◽  
Author(s):  
KH Talukder ◽  
IU Ahmed ◽  
MS Islam ◽  
MS Islam ◽  
M Asaduzzaman ◽  
...  

Fractions studies were done to know how the zinc applied to different soils was distributed in to various fractions when the soils incubated under aerobic and anaerobic condition. The added zinc provided significant increase in exchangeable Zn both under aerobic and anaerobic conditions although anaerobic condition gave lower results than aerobic condition. The higher results were obtained at early stage of incubation and it gradually reduced as the incubation period proceeded to 90 days. These results showed all most similar trends for all the soils under study. In general, added zinc showed significantly higher results to the different fractions of soil Zn both under anaerobic and aerobic incubation with very few exceptions. The highest amount of added Zn (12 kg/ha) always produced greater results than the lower doses. Only exchangeable Zn was found higher in the 1st measurement at 15 DAI then gradually decreased but in other cases, gradual increase in zinc fractions was seen as the incubation study proceed to longer duration provided with very few exceptions. In many cases, the exchangeable-Zn found higher only at 15 DAI but sharply reduced at 30 DAI. In general, the Gray Terrace Soil produced the highest results followed by Non Calcareous Gray Floodplain and the lowest results were observed in Dark Grey Floodplain & Brown Floodplain Soil. DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14643 J. Sci. Foundation, 9(1&2): 9-15, June-December 2011


2007 ◽  
Vol 72 (3) ◽  
pp. 420-431 ◽  
Author(s):  
Didier Jézéquel ◽  
Roberta Brayner ◽  
Edouard Metzger ◽  
Eric Viollier ◽  
François Prévot ◽  
...  

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