scholarly journals INTERACTION OF IRON(II) MIXED-LIGAND COMPLEXES WITH DNA: BASE-PAIR SPECIFICITY AND THERMAL DENATURATION STUDIES

2010 ◽  
Vol 4 (3) ◽  
pp. 174-179 ◽  
Author(s):  
Mudasir Mudasir ◽  
Karna Wijaya ◽  
Endang Tri Wahyuni ◽  
Hidenari Inoue
Keyword(s):  
Dna Binding ◽  
Electrostatic Interaction ◽  
Base Pair ◽  
Thermal Denaturation ◽  
Spectrophotometric Titration ◽  
Mixed Ligand ◽  
Mixed Ligand Complexes ◽  
Dna Base ◽  
Dna Base Pair ◽  
Ct Dna

A research about base-pair specificity of the DNA binding of [Fe(phen)3]2+, [Fe(phen)2(dip)]2+ and [Fe(phen)(dip)2]2+ complexes and the effect of calf-thymus DNA (ct-DNA) binding of these metal complexes on thermal denaturation of ct-DNA has been carried out. This research is intended to evaluate the preferential binding of the complexes to the sequence of DNA (A-T or G-C sequence) and to investigate the binding strength and mode upon their interaction with DNA. Base-pair specificity of the DNA binding of the complexes was determined by comparing the equilibrium binding constant (Kb) of each complex to polysynthetic DNA that contain only A-T or G-C sequence. The Kb value of the interaction was determined by spectrophotometric titration and thermal denaturation temperature (Tm) was determined by monitoring the absorbance of the mixture solution of each complex and ct-DNA at λ =260 nm as temperature was elevated in the range of 25 - 100 oC. Results of the study show that in general all iron(II) complexes studied exhibit a base-pair specificity in their DNA binding to prefer the relatively facile A-T sequence as compared to the G-C one. The thermal denaturation experiments have demonstrated that Fe(phen)3]2+ and [Fe(phen)2(dip)]2+ interact weakly with double helical DNA via electrostatic interaction as indicated by insignificant changes in melting temperature, whereas [Fe(phen)2(dip)]2+  most probably binds to DNA in mixed modes of interaction, i.e.: intercalation and electrostatic interaction. This conclusion is based on the fact that the binding of [Fe(phen)2(dip)]2+ to ct-DNA moderately increase the Tm value of ct- DNA   Keywords: DNA Binding, mixed-ligand complexes

DNA-Binding Properties of Iron(II) Mixed-Ligand Complexes Containing 1,10-Phenanthroline and Dipyrido[3,2-a:2’,3’-c]phenazine

2004 ◽  
Vol 59 (3) ◽  
pp. 310-318 ◽  
Author(s):  
◽  
Karna Wijaya ◽  
Daryono H. Tjahjono ◽  
Naoki Yoshioka ◽  
Hidenari Inoue
Keyword(s):  
Dna Binding ◽  
Bathochromic Shift ◽  
Mixed Ligand Complex ◽  
Visible Region ◽  
Spectrophotometric Titration ◽  
Mixed Ligand ◽  
Ligand Complex ◽  
Binding Properties ◽  
Ct Dna ◽  
Dna Binding Properties

An iron(II) mixed-ligand complex with 1,10-phenanthroline (phen) and dipyrido[3,2-a:2’,3’- c]phenazine (dppz), [Fe(phen)2(dppz)]2+, has been synthesized. The DNA-binding properties of the mixed-ligand complex have been studied in terms of equilibrium binding constant, thermodynamic parameter, thermal denaturation as well as Pfeiffer effect upon binding to DNA. The spectrophotometric titration of [Fe(phen)2(dppz)]2+ with calf thymus DNA (ct-DNA) has shown that the iron(II) mixed-ligand complex binds effectively to ct-DNA in an intercalation mode as indicated by remarkable hypochromicity (ca. 36%) and moderate bathochromic shift (8 nm) of the absorption spectra. This intercalative mode is supported by a significant increase (Δ Tm = 21 °C) in the melting temperature (Tm) of ct-DNA at R([complex]/[ct-DNA]) = 1.5. The binding of [Fe(phen)2(dppz)]2+ to ct-DNA is entropically driven as characterized by a positive enthalpy change and a large negative TΔ S term. An intense CD signal in the UV and visible region develops upon addition of ct-DNA to the racemate solution of [Fe(phen)2(dppz)]2+. This has revealed that a shift in diastereomeric inversion equilibrium takes place in the solution to yield an excess of one enantiomer of the DNA-iron(II) complex (Pfeiffer effect). The striking resemblance of the CD spectral profiles to those of the corresponding Δ -enantiomer indicates that Δ -[Fe(phen)2(dppz)]2+ is preferentially bound to ct-DNA

Varying DNA Base-Pair Size in Subangstrom Increments:  Evidence for a Loose, Not Large, Active Site in Low-Fidelity Dpo4 Polymerase

Biochemistry ◽  
2006 ◽  
Vol 45 (9) ◽  
pp. 2772-2778 ◽  
Author(s):  
Shin Mizukami ◽  
Tae Woo Kim ◽  
Sandra A. Helquist ◽  
Eric T. Kool
Keyword(s):  
Active Site ◽  
Base Pair ◽  
Dna Base ◽  
Dna Base Pair

scholarly journals A rhodium(III) complex for high-affinity DNA base-pair mismatch recognition

2003 ◽  
Vol 100 (7) ◽  
pp. 3737-3742 ◽  
Author(s):  
H. Junicke ◽  
J. R. Hart ◽  
J. Kisko ◽  
O. Glebov ◽  
I. R. Kirsch ◽  
...  
Keyword(s):  
Base Pair ◽  
High Affinity ◽  
Base Pair Mismatch ◽  
Dna Base ◽  
Mismatch Recognition ◽  
Dna Base Pair

Interaction between the Guanine−Cytosine Watson−Crick DNA Base Pair and Hydrated Group IIa (Mg2+, Ca2+, Sr2+, Ba2+) and Group IIb (Zn2+, Cd2+, Hg2+) Metal Cations

1998 ◽  
Vol 102 (29) ◽  
pp. 5951-5957 ◽  
Author(s):  
Jiří Šponer ◽  
Jaroslav V. Burda ◽  
Michal Sabat ◽  
Jerzy Leszczynski ◽  
Pavel Hobza
Keyword(s):  
Base Pair ◽  
Metal Cations ◽  
Dna Base ◽  
Dna Base Pair

DNA Base Pair Stacking Crystallization of Gold Colloids

Langmuir ◽  
2020 ◽  
Vol 36 (19) ◽  
pp. 5118-5125 ◽  
Author(s):  
Jaewon Lee ◽  
Ji-Hyeok Huh ◽  
Seungwoo Lee
Keyword(s):  
Base Pair ◽  
Gold Colloids ◽  
Dna Base ◽  
Dna Base Pair ◽  
Base Pair Stacking

scholarly journals Isosteric and fluorescent DNA base pair formed by 4-amino-phthalimide and 2,4-diaminopyrimidine: melting, structure, and THz polar solvation dynamics

2016 ◽  
Vol 18 (9) ◽  
pp. 6813-6820 ◽  
Author(s):  
L. Dehmel ◽  
F. Berndt ◽  
M. Weinberger ◽  
M. Sajadi ◽  
I. Ioffe ◽  
...  
Keyword(s):  
Base Pair ◽  
Solvation Dynamics ◽  
Duplex Dna ◽  
Dna Base ◽  
Dna Base Pair ◽  
Polar Solvation

An artificial base pair in duplex DNA, formed by 2,4-diaminopyrimidine and fluorescent 4-aminophthalimide C-nucleosides, is characterized spectroscopically, for use in femtosecond solvation dynamics.

140 Attacking mechanism of H and OH radicals to DNA base pair: DFT calculations in water

2015 ◽  
Vol 33 (sup1) ◽  
pp. 91-92
Author(s):  
Naoko Okutsu ◽  
Eisuke Shimizu ◽  
Victor I. Danilov ◽  
Sergiy Shulga ◽  
Noriyuki Kurita
Keyword(s):  
Dft Calculations ◽  
Base Pair ◽  
Oh Radicals ◽  
Dna Base ◽  
Dna Base Pair
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