scholarly journals SPECTROSCOPICAL STUDY OF AN ETHER FRACTION ISOLATE FROM STEAM BARK OF “ JATI” (Tectona grandis, L)

2010 ◽  
Vol 9 (2) ◽  
pp. 312-314
Author(s):  
Fredryk Mandey ◽  
Raimundus Chalik ◽  
Ismail Ibrahim
Keyword(s):  
Melting Point ◽  
Column Chromatography ◽  
Betulinic Acid ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Tectona Grandis ◽  
Stem Bark ◽  
H Nmr ◽  
Ether Fraction ◽  
Spectroscopical Study

This research is aimed to primarily study the spectroscopy of ether fraction of from Jati wood (T. grandis, L). The stem bark of T.grandis, L was firstly extracted undergoes a reflux in methanol solvent. Further, the dried methanol crude extract was extracted with diethyl ether and moreover separated its chemical constituents by using of column chromatography using degraded mixtures of n-hexane - ethyl acetate from 9:1 to 7:3. After a final column chromatography from the E fraction, a white needles crystal with a melting point of 295 - 297.5 °C was resulted. This results was then confirmed undergoes a spectroscopic analysis including UV, FTIR, H-NMR, and Mass spectroscopy to get an acid of 3β-hydroxy-20(29)-lupen-28-oic or commonly known as betulinic acid.   Keywords: betulinic acid, spectroscopy, primarily study

scholarly journals Anti-inflammatory and cytotoxic activities of the extracts, fractions, and chemical constituents isolated from Luehea ochrophylla Mart

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Clináscia Rodrigues Rocha Araújo ◽  
Thiago de Melo Silva ◽  
Michaelle Geralda dos Santos ◽  
Marcelo Henrique Fernandes Ottoni ◽  
Elaine Maria de Souza Fagundes ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cytotoxic Activity ◽  
Rheumatic Diseases ◽  
Betulinic Acid ◽  
Muscle Injury ◽  
Chemical Constituents ◽  
Stem Bark ◽  
Proliferation Index ◽  
Hct 116 ◽  
Anti Inflammatory

Abstract Background Stem bark of Luehea ochrophylla (L. ochrophylla) is used by the traditional Brazilian medicine for treatment of rheumatic diseases and tumors. This study aimed to investigate inhibition of acute and chronic inflammations and cytotoxic activity of extracts, fractions, and isolated compounds from L. ochrophylla. Methods Hexane (HE) and ethanol (EE) extracts obtained from stem bark of L. ochrophylla were submitted to chromatographic fractionation. In order to test acute inflammation, experimental model of impact injury was used, followed by transdermal application of gels using phonophoresis. Histological analysis was based on scores assigned by the capacity of decreasing the lesion. To evaluate the effect EE and fractions on cell proliferation, human lymphocytes were stimulated with phytohemagglutinin and analyzed using flow cytometry. Proliferation was measured using VPD 450 staining and the calculated proliferative index (PI). The cytotoxic activity was evaluated using MTT colorimetric method against MDA-MB-231, MCF-7, HCT-116, and Vero cells. GraphPad Prism Version 5 was used for statistical analysis. Results HE and EE provided friedelin, β-friedelinol, lupeol, mixture of lupeol and pseudotaraxasterol, β-sitosterol, betulinic acid, mixture of lupeol and taraxasterol, (−)-epicatechin, β-sitosterol-3-O-β-D-glucopyranoside, and (+)-epicatechin-(4β-8)-epicatechin. HE, ethyl acetate fraction (AF), betulinic acid, and β-sitosterol promoted regeneration of muscle fibers caused by muscle injury. AF significantly (p < 0.05) reduced the lymphocyte proliferation index (1.36 for cultures stimulated with PHA, 0.7 for untreated cultures and 0.12 for cultures stimulated with PHA and treated with AF 25 μg/mL and AF 50 μg/mL, respectively). β-Sitosterol-3-O-β-D-glucopyranoside exhibited high cytotoxic activity (IC50 = 1.279 μg/mL) against HCT-116 cell line. Conclusion These results suggest that extracts, fractions, and chemical constituents from L. ochrophylla decreases inflammatory processes generated by muscle injury. The anti-inflammatory activity may be justified by high inhibition of T cell proliferation. These extracts, fractions, and chemical constituents from L. ochrophylla may be useful as a therapeutic agent against rheumatic diseases. Moreover, chemical constituents from L. ochrophylla show potent cytotoxic activity against colon and rectal carcinomas.

Chemical constituents of the stem bark of Acacia AuriculiformisA.Cunn ex. Benth. (fabaceae)

2020 ◽  
Vol 23 ◽  
pp. 88-91
Author(s):  
A.A. Ahmadu ◽  
B.A. Lawal ◽  
B. Olanipekun ◽  
A. Udobre ◽  
N. Tsafantakis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Metabolites ◽  
Acid Ester ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Stem Bark ◽  
Acacia Auriculiformis ◽  
Compound I ◽  
Literature Report ◽  
First Time

The genus Acacia has been known to be a rich source of many secondary metabolites. This study was carried to isolate chemical constituents present in the stem bark of Acacia auriculiformis. The dichloromethane extract of the stem bark of Acacia auriculiformis was obtained by maceration. The extract obtained was subjected to silica gel column chromatography and preparative TLC. The isolated compounds were identified by spectroscopic analysis. This led to the isolation of ferulic acid ester (I), along with a steroid (II) and a triterpenoid (III). The structure of compound I was established using spectroscopic analysis (UV, IR, NMR and mass spectrometry) and identified to be dodecyl-4-hydroxy-3-methoxy-trans-cinnamate (I), compounds II and III were found to be á-spinasterol and lupenol respectively, based on the comparison of their spectral data NMR and MS with literature report. Compound I is being reported for the first time in the genus Acacia.

Chemical Constituents from the Seed of the Stellera chamejasme L

2013 ◽  
Vol 726-731 ◽  
pp. 50-53
Author(s):  
Chun Yu Wu ◽  
Fu Li Tian ◽  
Lei Wang ◽  
Hai Tian Yu
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Spectroscopic Analysis ◽  
Chemical Constituents

To investigate the chemical constituents of the seed of Stellera Chamejasme L, the constituents were isolated by silica gel, and polyamide column chromatography, and their structures were determined on the basis of spectroscopic analysis. Three compounds were obtained and identified as umbelliferone, 3-hydroxy-6-methoxy-7-(2-oxochromen-7-yl) oxychromen-2-one and dephnin. A novel compound two was isolated from the seed of Stellera Chamejasme L.

scholarly journals Triterpenoids from the Stem Bark of Avicennia officinalis

1970 ◽  
Vol 5 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Md. Enamul Haque ◽  
Hussain Uddin Shekhar ◽  
Akim Uddin Mohamad ◽  
Hafizur Rahman ◽  
AKM Mydul Islam ◽  
...  
Keyword(s):  
Thin Layer ◽  
Betulinic Acid ◽  
Full Text ◽  
Spectroscopic Analysis ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Stem Bark ◽  
Phytochemical Investigation ◽  
Avicennia Officinalis ◽  
Layer Chromatography

The triterpinoids, betulinic acid, lupeol and betulinaldehyde, were isolated from the ethyl acetate extract of the stem bark of Avicennia officinalis (Avicenniaceae) by a combination of column and preparative thin-layer chromatography over silica gel. The structures of these compounds were determined by spectroscopic analysis (UV, IR, 1H NMR, 13CNMR and EIMS). This is the first report of a systematic phytochemical investigation and the presence of these triterpoids from this plant. Key words: Triterpenoid, Avicenniaceae, Betulinic acid, Lupeol and Betulinaldehyde Dhaka Univ. J. Pharm. Sci. Vol.5(1-2) 2006 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals ISOLATION AND IDENTIFICATION FLAVONOID COMPOUND FROM THE STEM BARK OF Saccopetalum horsfieldii BENN

2010 ◽  
Vol 6 (3) ◽  
pp. 312-315
Author(s):  
Mahmiah Mahmiah
Keyword(s):  
Melting Point ◽  
1H Nmr ◽  
Column Chromatography ◽  
Room Temperature ◽  
Stem Bark ◽  
Spectroscopy Method ◽  
Flavonoid Compound ◽  
Isolation And Identification ◽  
Vacuum Column ◽  
Flash Column Chromatography

Isolation and identification flavonoid compound from the stem bark of Indonesian plant Saccopetalum horsfieldii Benn. Annonaceae family have been done. Extraction was carried out by maseration method using methanol at room temperature, the product was then extracted by n-hexane and etil acetate. Etil acetate extract separation carried out by liquid vacuum column chromatography and flash column chromatography. The product purificated by recristalization using acetone p.a. into yellow solid that having melting point of 224-226 °C. The structure of flavonoid compound was determinated by spectroscopy method : UV-vis, IR, 1H-NMR and 12C-NMR. The flavonoid compound known as quercetin-3,7-dimetil eter or 3,7-dimetoksi quercetin.                                                                                                                                                       Keywords: Saccopetalum horsfieldii Benn, flavonoid, chromatography, quercetin

ISOLASI SENYAWA KUMARIN DARI KULIT BATANG KECAPI (Sandoricum koetjape) SEBAGAI ANTIBAKTERI

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
FEBRIA ELVY SUSANTI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Melting Point ◽  
Ethyl Acetate ◽  
Organic Solvents ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Stem Bark ◽  
Antibacterial Activities ◽  
Sandoricum Koetjape

<p>Isolation of coumarine on stem bark from Sandoricum koetjape has done. Stem bark powder were extracted by maceration method using organic solvents hexane, ethyl acetate and methanol. Extract of ethyl acetate was separated by column chromatography. The antibacterial activities of extracts made by method of diffusion discs. The results showed the extracts of ethyl acetate has the highest activity than others with 12 mm (Staphylococcus aureus) and 19 mm (Escherichia coli) inhibitory area. Isolation result is needle white crystals from extracts of ethyl acetate with melting point 194ºC - 195ºC.</p>

Progesterone Side-Chain Cleavage by Aspergillus versicolor

2013 ◽  
Vol 781-784 ◽  
pp. 1164-1167
Author(s):  
Hai Ying Yang ◽  
Hai Ling Su ◽  
Gang Du ◽  
Guang Jian Shen ◽  
Jing Xian Sun ◽  
...  
Keyword(s):  
Melting Point ◽  
Column Chromatography ◽  
Optical Rotation ◽  
Side Chain ◽  
Spectroscopic Methods ◽  
Aspergillus Versicolor ◽  
Side Chain Cleavage ◽  
H Nmr ◽  
Chain Cleavage ◽  
C Nmr

Side chain cleavage of progesterone occurred in the biotransformation by Aspergillus versicolor. The conversion products were purified by column chromatography with ether/EtOAc and characterized by spectroscopic methods including 1H NMR, 13C NMR, IR, UV, MS and physical constants such as melting point and optical rotation. Those conversion products were identified as testosterone, androstenedione and androstenediendione.

scholarly journals Antioxidant Activity of the Isoflavonoids from the Roots of Maackia amurensis

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Nadezda I. Kulesh ◽  
Sergey A. Fedoreyev ◽  
Marina V. Veselova ◽  
Natalia P. Mischenko ◽  
Vladimir A. Denisenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Column Chromatography ◽  
Thiobarbituric Acid ◽  
Formalin Injection ◽  
Thiobarbituric Acid Reactive Substances ◽  
H Nmr ◽  
Maackia Amurensis

Seven isoflavonoids, including a new glycoside, (6a R,11a R)-medicarpin-3- O-gentiobioside (6), were isolated from the roots of Maackia amurensis using repeated column chromatography on a Toyopearl HW-50F sorbent and identified by HPLC–PDA–MS, 1H NMR, 13C, 1H–1H COSY, HSQC NMR and HMBC NMR analyses as daidzin (1), genistein-7- O-gentiobioside (2), pseudobaptigenin-7- O-gentiobioside (3), formononetin-7- O-gentiobioside (4), (6a R,11a R)-maackiain-3- O-gentiobioside (5), and 5- O-methylgenistein-7- O-gentiobioside (7). In the model of oxidative stress induced by formalin injection, the isolated isoflavone and pterocarpan glucosides 1-7 were shown to reduce the formation of malondialdehyde (MDA) and other thiobarbituric acid reactive substances (TBARS), as well as glutathione peroxidase (GPO) activity in rats.

scholarly journals Bioactive Antidiabetic Flavonoids from the Stem Bark of Cordia dichotoma Forst.: Identification, Docking and ADMET Studies

Molbank ◽  
10.3390/m1234 ◽  
2021 ◽  
Vol 2021 (2) ◽  
pp. M1234
Author(s):  
Nazim Hussain ◽  
Bibhuti Bhushan Kakoti ◽  
Mithun Rudrapal ◽  
Khomendra Kumar Sarwa ◽  
Ismail Celik ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Binding Affinity ◽  
Mass Spectroscopy ◽  
Column Chromatography ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Computational Studies ◽  
Autodock Vina ◽  
Online Tool

Cordia dichotoma Forst. (F. Boraginaceae) has been traditionally used for the management of a variety of human ailments. In our earlier work, the antidiabetic activity of methanolic bark extract of C. dichotoma (MECD) has been reported. In this paper, two flavonoid molecules were isolated (by column chromatography) and identified (by IR, NMR and mass spectroscopy/spectrometry) from the MECD with an aim to investigate their antidiabetic effectiveness. Molecular docking and ADMET studies were carried out using AutoDock Vina software and Swiss ADME online tool, respectively. The isolated flavonoids were identified as 3,5,7,3′,4′-tetrahydroxy-4-methoxyflavone-3-O-L-rhamnopyranoside and 5,7,3′-trihydroxy-4-methoxyflavone-7-O-L-rhamnopyranoside (quercitrin). Docking and ADMET studies revealed the promising binding affinity of flavonoid molecules for human lysosomal α-glucosidase and human pancreatic α-amylase with acceptable ADMET properties. Based on computational studies, our study reports the antidiabetic potential of the isolated flavonoids with predictive pharmacokinetics profile.

Non-destructive estimation of fibre morphological parameters and chemical constituents of Tectona grandis L.f. wood by near infrared spectroscopy

2021 ◽  
pp. 096703352199911
Author(s):  
SR Shukla ◽  
S Shashikala ◽  
M Sujatha
Keyword(s):  
Near Infrared ◽  
Cross Validation ◽  
Chemical Constituents ◽  
Fibre Diameter ◽  
Tectona Grandis ◽  
Cost Effective ◽  
Wood Products ◽  
Timber Species ◽  
Morphological Parameters ◽  
Mean Square Errors

Near infrared (NIR) spectroscopy is developing as an advanced and non-invasive tool in the wood, wood products and forestry sectors. It may be applied as a rapid and cost effective technique for assessment of different wood quality parameters of timber species. In the present study, NIR spectra of heartwood samples of Tectona grandis (teak) were collected before measuring fibre morphological parameters (fibre length, fibre diameter and fibre lumen diameter)and main chemical constituents (cellulose, hemicellulose, lignin and extractives) using maceration and wet chemistry methods respectively. Multivariate partial least squares (PLS) regression was applied to develop the calibration models between measured values of wood parameters and NIR spectral data. Pre-processing of NIR spectra demonstrated better predictions based on higher values of correlation coefficient for estimation (R2), validation (Rcv 2 ), ratio of performance to deviation (RPD), and lower values of root mean square errors of estimation (RMSEE), cross-validation (RMSECV) and number of latent variable (rank). Internal cross-validation was used to find the optimum rank. Robust calibrations models with high R2 (>0.87), low errors and high RPD values (> 2.93) were observed from PLS analysis for fibre morphological parameters and main chemical constituents of teak. These linear models may be applied for rapid and cost effective estimation of different fibre parameters and chemical constituents in routine testing and evaluation procedures for teak.

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