scholarly journals Cytotoxic Isobractatin (Prenylated Xanthone) Epimer Mixture of Garcinia eugenifolia

2014 ◽  
Vol 14 (3) ◽  
pp. 277-282 ◽  
Author(s):  
Sri Hartati ◽  
I Ketut Triyono ◽  
Sri Handayani
Keyword(s):  
Breast Cancer ◽  
Natural Products ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Anti Cancer ◽  
Prenylated Xanthone

In search of bioactive anti-cancer compounds from natural products has been found isobractatin from n-hexane extract of Garcinia eugenifolia. Isolation was done through the stages of maceration and column chromatography. Elucidation of the structure was analyzed by spectroscopy of UV-Vis, FTIR, LC-MS, NMR proton and carbon one and two dimension data. The results found three compounds isolated from G. eugenifolia. The three compounds were stigmasterol, prenylated xanthone isobractatin and its epimer (in a mixture). The mixture of isobractatin and its epimer showed cytotoxic activity against breast cancer cell line T47D with IC50 = 17 µg/mL.

scholarly journals EXTRACTION OF PHENOLIC COMPOUNDS AS ANTIOXIDANTS FROM SOME PLANTS AND THEIR CYTOTOXIC ACTIVITY AGAINST BREAST CANCER CELL LINE

2017 ◽  
Vol 10 (7) ◽  
pp. 349
Author(s):  
Rabab Omran ◽  
Zahraa Mal Taee ◽  
Mohammed Jal Jassani ◽  
Hayder O Hashim
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Normal Cell ◽  
Cancer Cell Line ◽  
Mcf 7

Objective: The objective of the study was to study the antioxidants and cytotoxic activities of phenolic extracts of some plants against breast cancer and normal cell lines.Methods: Phenolics were extracted from different parts of some plants (15) such as seeds, fruits, leaves, and rhizomes using methanol: ethanol: HCl: Distilled water at a ratio 50:29:1:20 (v:v:v:v). The contents of phenolics and flavonoids were estimated using gallic acid and quercetin as standards, respectively. The antioxidant activity was determined by 2,2ˋ-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. Phenolic flavonoids were partially purified by adsorption chromatography using a silica gel column from selected plants and assayed their cytotoxic activity against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and a normal cell line of non-tumorigenic fetal hepatic cell line (WRL-68).Results: The Cinnamomum zeylanicum extract had the highest phenolic and flavonoid contents were followed by Lawsonia inermis, Citrullus colocynthis, Syzygium aromaticum, Peganum nigrum, and Phoenix dactylifera. The antioxidant activity of Curcuma longa, P. dactylifera, C. colocynthis, Solanum melongena, and C. zeylanicum extracts had the highest ability to scavenge the free radicals. The acetone fraction of P. dactylifera and C. colocynthis extracts had the minimum inhibition dose that kills 50% of cells inhibitory concentration 50 values 156.91 μg and 1055.06 μg against MCF-7 and 372.86 μg and 153.8 μg against WRL-68, respectively. While the S. melongena extract had less effect on both cell lines.Conclusions: Phenolics as antioxidant substances had moderate or variable effectiveness on normal and cancer cell lines, and the highest concentrations were cancerous poison impact may be on normal cells over than cancer cells.

Sign in / Sign up

Export Citation Format

Share Document