scholarly journals Potassium Permanganate-Catalyzed Alpha-Pinene Oxidation: Formation of Coordination Compound with Zinc(II) and Copper(II), and Growth Inhibition Activity on Staphylococcus aureus and Escherichia coli

2018 ◽  
Vol 16 (1) ◽  
pp. 59 ◽  
Author(s):  
Masruri Masruri ◽  
Rekfa Wika Amini ◽  
Mohammad Farid Rahman

Catalytic oxidation of alpha-pinene was investigated using potassium permanganate as an oxidant. The reaction consumed catalyst following stoichiometric amount instead of the catalytic one. The keto-carboxylate compound 2 was afforded as the oxidation product. Further study of its complex compound with copper(II) and zinc(II) was also reported including their activity for inhibiting the growth of Staphylococcus aureus and Escherichia coli. In overall, the complex compound shows important result by inhibiting the bacterial growth.

2019 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Ariyetti Ariyetti ◽  
Muhammad Nasir ◽  
Safni Safni ◽  
Syukri Darajat

<p><em>Metil merah merupakan salah satu zat warna golongan azo yang sering digunakan dalam industri dan laboratorium. Penggunaan metil merah dapat menimbulkan efek terhadap kesehatan dan lingkungan. Oleh sebab itu dilakukan metode fotodegradasi dengan menggunakan semikonduktor dan radiasi sinar tampak. Semikonduktor yang digunakan yaitu berbahan dasar tembaga sulfat hidrat dan perak nitrat. Prekusor tembaga sulfat hidrat dibuat dari pengolahan limbah logam tembaga hasil pemotongan tembaga yang ada di bengkel Lembaga Ilmu Pengetahuan Indonesia (LIPI) Bandung. Bahan semikonduktor juga memiliki kemampuan dalam menghambat pertumbuhan bakteri. Hasil optimum yang didapatkan dalam proses fotodegradasi dan antibakteri merupakan gabungan antara kedua prekusor tembaga sulfat hidrat dan perak nitrat dengan bantuan penyinaran. Kemampuan dalam menghambat pertumbuhan bakteri didapatkan persentase kematian 100 % untuk masing-masing bakteri, yaitu Escherichia coli dan Staphylococcus aureus. Aktifitas fotokatalitiknya dengan konsentrasi semikonduktor 10 ppm untuk mendegradasi zat warna metil merah 5 ppm, selama 23 jam, dimana persentase degradasi yang didapatkan dengan penyinaran lebih tinggi dibandingkan dengan tanpa penyinaran. Pengaruh pH larutan terhadap degradasi metil merah yaitu optimum pada pH 12 (basa).</em></p><p><em><br /></em></p><p><em>Methyl red is one of the azo group dyes that is often used in industry and laboratories. The use of methyl red can have an effect on health and the environment. Therefore photodegradation method is done by using semiconductor and visible light radiation. The semiconductor used is based on copper sulfate hydrate and silver nitrate. The copper sulphate hydrate precursor is made from the processing of copper-cut copper metal waste in the workshop of the Indonesian Institute of Sciences (LIPI) in Bandung. Semiconductor materials also have the ability to inhibit bacterial growth. The optimum results obtained in the photodegradation and antibacterial process are a combination of both copper sulfate hydrate precursor and silver nitrate with the help of irradiation. The ability to inhibit bacterial growth obtained 100% mortality for each bacterium, namely Escherichia coli and Staphylococcus aureus. Photocatalytic activity with 10 ppm semiconductor concentration to degrade methyl red dye 5 ppm, for 23 hours, where the percentage of degradation obtained by irradiation is higher than without irradiation. The effect of pH of the solution on the degradation of methyl red is optimum at pH 12 (base).</em></p>


2020 ◽  
Vol 8 (10) ◽  
pp. 1478 ◽  
Author(s):  
Dobroslava Bujňáková ◽  
Anna Čuvalová ◽  
Milan Čížek ◽  
Filip Humenik ◽  
Michel Salzet ◽  
...  

The present study investigated the in vitro antibacterial, antibiofilm and anti-Quorum Sensing (anti-QS) activities of canine bone marrow mesenchymal stem cell-conditioned media (cBM MSC CM) containing all secreted factors <30 K, using a disc diffusion test (DDT), spectrophotometric Crystal Violet Assay (SCVA) and Bioluminescence Assay (BA) with QS-reporter Escherichia coli JM109 pSB1142. The results show a sample-specific bacterial growth inhibition (zones varied between 7–30 mm), statistically significant modulation of biofilm-associated Staphylococcus aureus and Escherichia coli bioluminescence (0.391 ± 0.062 in the positive control to the lowest 0.150 ± 0.096 in the experimental group, cf. 11,714 ± 1362 to 7753 ± 700, given as average values of absorbance A550 ± SD versus average values of relative light units to growth RLU/A550 ± SD). The proteomic analysis performed in our previous experiment revealed the presence of several substances with documented antibacterial, antibiofilm and immunomodulatory properties (namely, apolipoprotein B and D; amyloid-β peptide; cathepsin B; protein S100-A4, galectin 3, CLEC3A, granulin, transferrin). This study highlights that cBM MSC CM may represent an important new approach to managing biofilm-associated and QS signal molecule-dependent bacterial infections. To the best of our knowledge, there is no previous documentation of canine BM MSC CM associated with in vitro antibiofilm and anti-QS activity.


2004 ◽  
Vol 52 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Shigeki Kobayashi ◽  
Nahomi Atuchi ◽  
Hiroki Kobayashi ◽  
Akiko Shiraishi ◽  
Hajime Hamashima ◽  
...  

2015 ◽  
Vol 327 ◽  
pp. 504-516 ◽  
Author(s):  
D. Nithyadevi ◽  
P. Suresh Kumar ◽  
D. Mangalaraj ◽  
N. Ponpandian ◽  
C. Viswanathan ◽  
...  

1981 ◽  
Vol 27 (2) ◽  
pp. 216-225 ◽  
Author(s):  
Beatriz M. Nieves ◽  
Francisco Gil ◽  
Francisco J. Castillo

Thirty-four strains corresponding to 16 species of Clostridium were examined for growth inhibition activity and production of phage and bacteriocinlike particles. Twenty-five strains were found to inhibit growth of one or more of the other strains. The widest range of activity corresponded to C. bifermentans 18137 which inhibited growth of 23 strains. Cell-free filtrates of positive cultures were inactive with the exception of the one from C. bifermentans 18137. A wide variety of particles with diverse morphology was observed in lysates of cultures treated with mitomycin C (1 μg/mL).


2004 ◽  
Vol 13 (5) ◽  
pp. 282-296
Author(s):  
Romeo Romagnoli ◽  
Pier Giovanni Baraldi ◽  
Maria Giovanna Pavani ◽  
Francesca Fruttarolo ◽  
Delia Preti ◽  
...  

2003 ◽  
Vol 11 (6) ◽  
pp. 965-975 ◽  
Author(s):  
Pier Giovanni Baraldi ◽  
Italo Beria ◽  
Paolo Cozzi ◽  
Nicoletta Bianchi ◽  
Roberto Gambari ◽  
...  

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