scholarly journals Pengaruh Penambahan Laurat dan Glisin terhadap Nilai Warna dan Kadar Sitrinin Angkak

2019 ◽  
Vol 38 (3) ◽  
pp. 320
Author(s):  
Susana Ristiarini ◽  
Muhammad Nur Cahyanto ◽  
Jaka Widada ◽  
Endang Sutriswati Rahayu
Keyword(s):  
Fatty Acid ◽  
Polyketide Synthase ◽  
Ph Value ◽  
Water Soluble ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Significant Difference ◽  
Solids Content ◽  
And Control ◽  
Number Of Cells

Angkak, commonly used for food colorant and flavor enhancers in oriental cuisine, is the result of fermentation by Monascus purpureus on steamed rice. In addition to producing pigments Monascus purpureus, Angkak also produces mycotoxins, citrinin, which is hepato-nephrotoxic. Biosynthesis of pigment and citrinin is following a polyketide synthase pathway and then subdivides to form pigment or citrinin. Fatty acids and amino acids are known to be the precursors of red pigment formers in their biosynthetic pathways. The purpose of this research was to know the effect of addition of lauric fatty acid and amino acid glycine on steamed rice substrate to the color characteristic and citrinin level by M. purpureus JK9A. The amount of fatty acid and glycine was respectively 0.5% and 1% (w/w). Fermentation was carried out for 14 days and every two days the solids content of fermented products, pH, number of cells, colors, pigments dissolved in water were measured. The level of citrinin was tested at the end of the fermentation period (14th day). There was no significant difference between treatments for the solids content of about 23  ̶  29% and the number of cells 6.32  ̶  6.56 logCFU/g. While the pH value, color and water-soluble pigments were significantly different between treatment and control. The ˚hue values of glycine and combination of lauric-glycine were 16.11 and 15.33, respectively, lower than controls (22.76). The highest A500nm/A400nm ratio was in the combination treatment of lauric-glycine and the lowest levels of citrinin also in the treatment of lauric-glycine combination. This study noticed that the addition of lauric or glycine and its combination in rice media for Monascus purpureus JK9A fermentation proved to increase the biosynthesis of red pigment (46.34%) and decrease citrinin level up to 49.97%.

Some effects of Mermis nigrescens on the hemolymph of Schistocerca gregaria

1978 ◽  
Vol 56 (2) ◽  
pp. 339-347 ◽  
Author(s):  
T. A. Rutherford ◽  
J. M. Webster
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Schistocerca Gregaria ◽  
Infection Period ◽  
Glucose Levels ◽  
Lipid Fatty Acid ◽  
Significant Difference ◽  
And Control

The sugar, free amino acid, and lipid fatty acid components of the hemolymph of adult Schistocerca gregaria parasitized by the larvae of the entomophilic nematode Mermis nigrescens were examined at intervals during the 4-week infection period. Glucose levels remained low, relative to trehalose, and at no time during the infection was there a significant difference in the glucose concentrations of controls and parasitized locusts. Trehalose was maintained at high levels (75–80 mM) in controls but fell to less than 25 mM between 7 and 10 days after parasitization and continued at a low level until day 17, when it began to rise in the parasitized group. Total free amino acid levels doubled in the parasitized group by day 21. By day 7 the concentration of methionine doubled, by day 14 both methionine and ornithine were at high levels, and by day 21, 13 of the 21 ninhydrin-positive substances were different from controls. There was no significant difference in the relative lipid fatty acid composition at day 14 between parasitized and control locusts.It is suggested that the nematode modifies its host's metabolism to favour production of small molecules such as glucose and amino acids from carbohydrate and protein reserves and it is also suggested that the host cell's ability to sequester nutrients is impaired during parasitism.

scholarly journals Influence of breed on selected quality parameters of fresh goat meat

2020 ◽  
Vol 63 (2) ◽  
pp. 219-229
Author(s):  
Snežana Ivanović ◽  
Marija Pavlović ◽  
Ivan Pavlović ◽  
Aleksandra Tasić ◽  
Jelena Janjić ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Volatile Compounds ◽  
Ph Value ◽  
Quality Parameters ◽  
Saanen Goat ◽  
Goat Meat ◽  
Significant Difference ◽  
The Impact

Abstract. The potential of goats to produce a high-quality meat is mainly reflected in their healthy fats, low calorie intramuscular fats, saturated fats, and, especially, their high ratios of unsaturated (UFA) and saturated (SFA) fatty acids, as well as hypocholesterolemic and hypercholesterolemic fatty acids. The aim of this study was to collect and compare meat quality parameters for domestic Balkan, Alpine and Saanen goats of the same age. Samples for all tests were taken from musculus gluteus superficialis. Chemical composition, pH value, fatty acid composition, content of volatile compounds, color and overall sensory quality (appearance, texture and smell) were determined. In chemical composition, moisture, fat, protein and ash varied significantly between each of the examined groups as opposed to pH values. Furthermore, among all the examined groups a significant difference was found for fatty acids and volatile compounds. Determined ratio of polyunsaturated fatty acids (PUFAs) to SFAs was 0.089, 0.085 and 0.071 for Balkan, Alpine and Saanen goat meats, respectively. Regarding that ratio, Saanen goat meat had the most favorable characteristics. Saanen goat meat showed the highest nutritional value. On the other hand, Balkan goat meat had the lowest intramuscular fat content. Measurements of the meat color from all three groups, as well as overall acceptability, showed significant differences between breeds. Obtained results point to the impact of breed on chemical composition and fatty acid profile of goat meat.

scholarly journals Comparison of infertility of Varroa destructor in resistant and control colonies of the Iraqi honeybee (Apis mellifera meda)

Biologija ◽  
2020 ◽  
Vol 66 (3) ◽  
Author(s):  
Farzad Moradpour
Keyword(s):  
Varroa Destructor ◽  
Varroa Mite ◽  
Hygienic Behaviour ◽  
Significant Difference ◽  
Varroa Mites ◽  
Apis Mellifera Meda ◽  
The University ◽  
And Control ◽  
Number Of Cells ◽  
Mite Eggs

In the current study, the infertility of Varroa mite was compared in resistant (VSH) and control honeybee colonies at the University of Garmian of Iraq. At each stage, the infestation rate, the percentage of fertile mites, the percentage of infertile mites, the number of cells containing mite eggs, and the number of protonymphs and deutonymphs were counted. Percentages of infestation in resistant and control colonies were 6.2 ± 0.9 and 10.9 ± 1.2, respectively. There was a statistically significant difference in the percentage of infested cells between the control and resistant groups (p < 0.05). Moreover, 56.3% of the resistant colonies and 37.50% of the control colonies showed hygienic behaviour (VHS). Resistant colonies showed hygienic behaviour (VHS) regarding the removal of infested pupae and reducing the infestation in colonies compared to control colonies. Mean total infertility in resistant and control colonies were 1.4 ± 0.4 and 1.1 ± 0.3, respectively. Also, the total mean of protonymphs, deutonymphs, and eggs in resistant and control colonies were 0.06, 1.3 ± 0.4, and 2.6 ± 0.8 in resistant colonies and 0.2, 1.3 ± 0.8, and 2.6 ± 1 in control colonies. The results showed that VSH behaviour in adult bees reduces the rate of infestation by Varroa mites in pupas.

scholarly journals Manuka Honey with Varying Levels of Active Manuka Factor (AMF) Ratings as an Anaerobic Fermentation Substrate for Limosilactobacillus reuteri DPC16

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 128
Author(s):  
Anand Mohan ◽  
Noemi Gutierrez-Maddox ◽  
Tong Meng ◽  
Ning He ◽  
Yihuai Gao ◽  
...  
Keyword(s):  
Ph Value ◽  
Anaerobic Fermentation ◽  
Probiotic Strain ◽  
Short Chain Fatty Acids ◽  
Manuka Honey ◽  
Significant Difference ◽  
Batch Fermenter ◽  
And Control ◽  
Strong Antibacterial Effect ◽  
Fermentation Substrate

Manuka honey is known for its strong antibacterial effect against pathogens but can promote probiotic growth in certain conditions. In a two-factor ANOVA study, AMFTM Manuka honey (Active Manuka Factor: 05+, 10+, 15+ and 20+) was utilised as a substrate for probiotic Limosilactobacillus reuteri DPC16 in an anaerobic batch fermenter for 36 h. The biomass growth in MRS broth was noticeably higher with AMF Manuka honey than invert syrup and control samples without any additional sweetener source. The pH value was significantly lowered below 4.0 only in the AMF samples with the formation of lactic acid as the major metabolite. Other beneficial short-chain fatty acids (SCFA), such as acetic, succinic, and propionic acids, produced during the fermentation, along with the honey saccharides, were quantified by two-dimensional (2-D) nuclear magnetic resonance (NMR) spectroscopy. A significantly (p < 0.05) high biomass in AMF 20+ sample after 36 h, can partly be attributed to the high total sugar and oligosaccharide content in the honey. Importantly, however, no statistically significant difference was observed in the recorded major fermentation outcomes for the different AMF levels. The results, nevertheless, indicate the potential prebiotic efficacy of Manuka honey as a fermentation substrate for the lactobacilli probiotic strain.

scholarly journals Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)

2013 ◽  
Vol 11 (1) ◽  
pp. 193-198 ◽  
Author(s):  
Celina Alcoforado Santos ◽  
Dominik Lenz ◽  
Geisamanda Pedrini Brandão ◽  
Adriana Regina Chippari-Gomes ◽  
Levy Carvalho Gomes
Keyword(s):  
Soluble Fraction ◽  
Micronucleus Test ◽  
Exposure Period ◽  
Diesel Oil ◽  
Water Soluble ◽  
Control Fish ◽  
Control Group ◽  
Water Soluble Fraction ◽  
Significant Difference ◽  
And Control

Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.

scholarly journals OPTIMIZATION OF RED PIGMENT PRODUCTION BY MONASCUS PURPUREUS FTC 5356 USING RESPONSE SURFACE METHODOLOGY

2018 ◽  
Vol 19 (1) ◽  
pp. 34-47 ◽  
Author(s):  
Farhan M. Said ◽  
Nor Farhana Binti Hamid
Keyword(s):  
Response Surface Methodology ◽  
Moisture Content ◽  
Response Surface ◽  
Ph Value ◽  
Initial Moisture Content ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Design Expert ◽  
Initial Ph

Factors such as environmental conditions and nutrients are significant for successful growth and reproduction of microorganisms. Manipulations of the factors are the most effective way to stimulate the growth of the microorganism, which can be used to optimize the yield of a product. In this study, Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimize the production of red pigment by Monascus purpureus FTC 5356 using the petioles of oil palm fronds (OPF) as a substrate in solid state fermentation (SSF). The data was analyzed using Design Expert Software. The optimum combination predicted via RSM was confirmed through experimental work. The interactions between three variables such as initial moisture content (%), initial pH value (pH), and peptone concentration (%) were studied and modelled. The statistical analysis of the results showed that the optimal conditions for red pigment production 47 AU/g with the biomass of 425.1 mg/g was at 55% initial moisture content, 3% of peptone, and at pH 3.  The RSM results showed that the initial pH value had a significant effect on red pigment production (P-value <0.05). The validation of these results was also conducted by fermentation with predicted conditions and it was found that there was a discrepancy of 0.39% between the values of the experimental result and those of the predicted values. ABSTRAK: Keadaan persekitaran dan nutrien merupakan faktor-faktor penting dalam pertumbuhan mikroorganisma. Manipulasi faktor-faktor tersebut adalah kaedah terbaik bagi meningkatkan pertumbuhan mikroorganisma dan mengoptimumkan penghasilan produk. Kajian ini mengguna pakai Rekaan Gabungan Pusat (CCD) melalui Kaedah Tindak balas Permukaan (RSM) bagi penghasilan pigmen merah optimum oleh Monascus purpureus FTC 5356 menggunakan batang pelepah kelapa sawit (OPF) sebagai perumah dalam proses penapaian pepejal (SSF). Data telah dianalisis menggunakan perisian Design Expert. Gabungan parameter optimum seperti cadangan RSM telah disahkan secara eksperimen. Interaksi antara tiga pemboleh ubah seperti kandungan lembapan awal (%), nilai pH awal (pH), dan kepekatan pepton (%) telah dikaji dan dimodelkan. Analisis statistik menunjukkan penghasilan optimal pigmen merah adalah pada 47 AU/g dengan biomas sebanyak 425.1 mg/g, pada 55% lembapan awal, 3% pepton dan pada pH 3. Hasil keputusan RSM menunjukkan pH awal memberikan kesan signifikan kepada penghasilan pigmen merah (nilai P <0.05). Pengesahan analisis juga telah dijalankan melalui proses penapaian dan hasil ujikaji mendapati 0.39% lebih tinggi daripada nilai jangkaan.

IOP Lowering Effects of Ocimum basilicum Seed Extract in Two Rabbit Models of Ocular Hypertension

2019 ◽  
Vol 4 (1) ◽  
pp. 39
Author(s):  
Renu Agarwal ◽  
SK Gupta ◽  
Sushma Srivastava ◽  
Rohit Saxena
Keyword(s):  
Ocular Hypertension ◽  
Antioxidant Properties ◽  
Ocimum Basilicum ◽  
Seed Extract ◽  
Water Loading ◽  
Antiglaucoma Medication ◽  
Lowering Effect ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Introduction: Ocimum basilicum (OB), a herb known for its antihypertensive, anticholinesterase and antioxidant properties was investigated for possible intraocular pressure (IOP) lowering effects in rabbits with ocular hypertension (OHT). Methods: The IOP lowering effect of a single drop of OB extract (OBE) was evaluated in oculonormotensive rabbits using three concentrations (0.25, 0.5 and 1% w/v). The concentration showing maximum IOP reduction was further evaluated in rabbits with water-loading and steroid-induced OHT. Results: IOP lowering effect of OBE 0.5% in oculonormotensive rabbit eyes was significantly greater compared to OBE 0.25% (p<0.05) but was comparable (p>0.05) to OBE 1%. Therefore, 0.5% concentration was selected for further evaluation. Pretreatment with OBE (0.5%) caused significantly lower increase in IOP after water loading amounting to 23.39% above baseline as compared to 54.00% in control eye, 15 minutes post water loading. At 60 minutes, post water loading, mean IOP rise was 95.12% and 63.58% in control and test eyes, respectively. Significant difference between the mean IOP of two eyes persisted during the 2nd hr. In rabbits with steroid induced OHT, OBE 0.5% produced a mean IOP reduction of 24.73% at the end of first hr and the mean peak IOP reduction of 31.63% was observed at the end of 2 hr. A significant difference between the IOP of test and control eyes persisted from 1 to 6 hr. Conclusions: Ocimum basilicum seed extract showed significant IOP lowering effect in rabbits with water loading and steroid induced OHT, however, its utility as an effective antiglaucoma medication needs further investigations.

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim
Keyword(s):  
Allergic Rhinitis ◽  
Side Effects ◽  
Coconut Oil ◽  
Test Group ◽  
Nasal Symptom ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

THE EFFECT OF THE ATTITUDE TOWARD BREASTFEEDING CONCEPT BASED COMIC ON THE ADOLESCENCE'S ATTITUDE TOWARD EXCLUSIVE BREASTFEEDING

2017 ◽  
Vol 1 (2) ◽  
pp. 138
Author(s):  
Mekar Dwi Anggraeni ◽  
Lutfatul Latifah ◽  
Aprilia Kartikasari ◽  
Ima Rismawati
Keyword(s):  
Exclusive Breastfeeding ◽  
Nuclear Family ◽  
T Test ◽  
Breastfeeding Duration ◽  
Control Group ◽  
Marriage Age ◽  
First Marriage ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Background and purpose: The earlier development of the attitude toward exclusive breastfeeding produces the longer exclusive breastfeeding duration. Considering the first marriage age among Indonesian, the attitude toward exclusive breastfeeding should be developed at the adolescence age. The purpose of this study was to examine the effect of the attitude toward breastfeeding concept based comic on the adolescent's attitude toward exclusive breastfeeding. Method: This was a quasy experimental posttest only with control group study. The respondents were provided an comic. The respondent's attitude toward exclusive breastfeeding was measured using The Breastfeeding Attitude Questionnaire. Data were analyzed using independent and dependent t test. Results: The majority of respondents were aged 17 years old in both intervention (70%) and control grup (63%), first child in both intervention (23,3%) and control grup (26,7%), and had a nuclear family in both intervention (80%) and control grup (90%). The independent t test showed that there was a significant difference between post-test scores among the intervention and control groups (t = 5,602, p < 0,01). Conclusion and recommendation: Nurses may use the Attitude Toward Breastfeeding based comic to increase the Adolescence's attitude toward breastfeeding.Keywords: Comic, Attitudes Toward Exclusive Breastfeeding, Adolescence

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