scholarly journals BACTERIAL COMMUNITY COMPOSITION MONITORING FOR THE IMPACT OF COMBINED SEWER OVER-FLOW INTO HIGASHIYOKOBORI-GAWA WATERWAY, AND ITS REMEDIAL EFFECTS BY BUILDING A STORMWATER STORAGE PIPE

2017 ◽  
Vol 73 (3) ◽  
pp. 134-142 ◽  
Author(s):  
Kazuaki MATSUI ◽  
Yuichi YOKOYAMA ◽  
Kunpei KAMEI ◽  
Yuzuru NAKAGUCHI ◽  
Mitsuru EGUCHI ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Combined Sewer ◽  
The Impact ◽  
Composition Monitoring

scholarly journals Anthropogenic Impact on Tropical Perennial River in South India: Snapshot of Carbon Dynamics and Bacterial Community Composition

Water ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1354
Author(s):  
Katrin Premke ◽  
Gunasekaran Dharanivasan ◽  
Kristin Steger ◽  
Kai Nils Nitzsche ◽  
Vijayan Jayavignesh ◽  
...  
Keyword(s):  
Water Quality ◽  
Bacterial Community ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Cauvery River ◽  
Main River ◽  
Warning Limits ◽  
Induced Changes ◽  
Riverine Systems ◽  
The Impact

Riverine systems play an important role in the global carbon cycle, and they are considered hotspots for bacterial activities such as organic matter decomposition. However, our knowledge about these processes in tropical or subtropical regions is limited. The aim of this study was to investigate anthropogenically induced changes of water quality, the distribution of selected pharmaceuticals, and the effects of pollution on greenhouse gas concentrations and bacterial community composition along the 800 km long Cauvery river, the main river serving as a potable and irrigation water supply in Southern India. We found that in situ measured pCO2 and pCH4 concentrations were supersaturated relative to the atmosphere and ranged from 7.9 to 168.7 μmol L−1, and from 0.01 to 2.76 μmol L−1, respectively. Pharmaceuticals like triclosan, carbamazepine, ibuprofen, naproxen, propylparaben, and diclofenac exceeded warning limits along the Cauvery. Proteobacteria was the major phylum in all samples, ranging between 26.1% and 82.2% relative abundance, and it coincided with the accumulation of nutrients in the flowing water. Results emphasized the impact of industrialization and increased population density on changes in water quality, riverine carbon fluxes, and bacterial community structure.

scholarly journals Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans

2015 ◽  
Vol 81 (23) ◽  
pp. 8224-8232 ◽  
Author(s):  
Eva Teira ◽  
Víctor Hernando-Morales ◽  
Francisco M. Cornejo-Castillo ◽  
Laura Alonso-Sáez ◽  
Hugo Sarmento ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Nucleic Acid Content ◽  
Carbon Conversion ◽  
Production Rates ◽  
Conversion Factors ◽  
Content Type ◽  
Carbon Production ◽  
The Impact

ABSTRACTThe transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the world's oceans, where the growth of the natural prokaryotic assemblages was promoted by filtration (i.e., removal of grazers [F treatment]) or filtration combined with dilution (i.e., also relieving resource competition [FD treatment]). The impact of sunlight exposure was also evaluated in the FD treatments, and we did not find a significant effect on the eCFs. The eCFs varied from 0.09 to 1.47 kg C mol Leu−1and were significantly lower in the FD than in the F samples. Also, changes in bacterial community composition during the incubations, as assessed by automated ribosomal intergenic spacer analysis (ARISA), were more pronounced in the FD than in the F treatments, compared to unmanipulated controls. Thus, we discourage the common procedure of diluting samples (in addition to filtration) for eCF determination. The eCFs in the filtered treatment were negatively correlated with the initial chlorophyllaconcentration, picocyanobacterial abundance (mostlyProchlorococcus), and the percentage of heterotrophic prokaryotes with high nucleic acid content (%HNA). The latter two variables explained 80% of the eCF variability in the F treatment, supporting the view that bothProchlorococcusand HNA prokaryotes incorporate leucine in substantial amounts, although this results in relatively low carbon production rates in the oligotrophic ocean.

scholarly journals Fecal Bacterial Community of Allopatric Przewalski’s Gazelles and Their Sympatric Relatives

2021 ◽  
Vol 12 ◽  
Author(s):  
Ruoshuang Liu ◽  
Jianbin Shi ◽  
Susanne Shultz ◽  
Dongsheng Guo ◽  
Dingzhen Liu
Keyword(s):  
Bacterial Community ◽  
Community Composition ◽  
Bacterial Communities ◽  
Bacterial Community Composition ◽  
Ecological Factors ◽  
Ovis Aries ◽  
Host Community ◽  
Tibetan Sheep ◽  
Fecal Bacterial Community ◽  
The Impact

Mammal gastrointestinal tracts harbor diverse bacterial communities that play important roles in digestion, development, behavior, and immune function. Although, there is an increasing understanding of the factors that affect microbial community composition in laboratory populations, the impact of environment and host community composition on microbiomes in wild populations is less understood. Given that the composition of bacterial communities can be shaped by ecological factors, particularly exposure to the microbiome of other individuals, inter-specific interactions should impact on microbiome community composition. Here, we evaluated inter-population and inter-specific similarity in the fecal microbiota of Przewalski’s gazelle (Procapra przewalskii), an endangered endemic ruminant around Qinghai Lake in China. We compared the fecal bacterial communities of three Przewalski’s gazelle populations, with those of two sympatric ruminants, Tibetan gazelle (Procapra picticaudata) and Tibetan sheep (Ovis aries). The fecal bacterial community richness (Chao1, ACE) did not vary across the three Przewalski’s gazelle populations, nor did the composition vary between species. In contrast, the managed Przewalski’s gazelle population had higher bacterial diversity (Shannon and Simpson) and was more similar to its sympatric Tibetan sheep in beta diversity than the wild Przewalski’s gazelle populations. These results suggest that ecological factors like host community composition or diet affect Przewalski’s gazelle’s gastrointestinal bacterial community. The role of bacterial community composition in maintaining gastrointestinal health should be assessed to improve conservation management of endangered Przewalski’s gazelle. More broadly, captive breeding and reintroduction efforts may be impeded, where captive management results in dysbiosis and introduction of pathogenic bacteria. In free ranging populations, where wildlife and livestock co-occur, infection by domestic pathogens and diseases may be an underappreciated threat to wild animals.

scholarly journals Quantitative and qualitative evaluation of the impact of the G2 enhancer, bead sizes and lysing tubes on the bacterial community composition during DNA extraction from recalcitrant soil core samples based on community sequencing and qPCR

2018 ◽  
Author(s):  
Alex Gobbi ◽  
Rui G. Santini ◽  
Elisa Filippi ◽  
Lea Ellegaard- Jensen ◽  
Carsten S. Jacobsen ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Dna Extraction ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Microbial Community Composition ◽  
Soil Core ◽  
Soil Matrix ◽  
Soil Dna ◽  
Dna Recovery ◽  
The Impact

AbstractSoil DNA extraction encounters numerous challenges that can affect both yield and purity of the recovered DNA. Clay particles lead to reduced DNA extraction efficiency, and PCR inhibitors from the soil matrix can negatively affect downstream analyses when applying DNA sequencing. Further, these effects impede molecular analysis of bacterial community compositions in lower biomass samples, as often observed in deeper soil layers. Many studies avoid these complications by using indirect DNA extraction with prior separation of the cells from the matrix, but such methods introduce other biases that influence the resulting microbial community composition.To address these issues, a direct DNA extraction method was applied in combination with the use of a commercial product, the G2 DNA/RNA Enhancer®, marketed as being capable of improving the amount of DNA recovered after the lysis step. The results showed that application of G2 increased DNA yields from the studied clayey soils from layers between 1.00 and 2.20 m below ground level.Importantly, the use of G2 did not introduce bias, as it did not result in any significant differences in the biodiversity of the bacterial community measured in terms of alpha and beta diversity and taxonomical composition.Finally, this study considered a set of customised lysing tubes for evaluating possible influences on the DNA yield. Tubes customization included different bead sizes and amounts, along with lysing tubes coming from two suppliers. Results showed that the lysing tubes with mixed beads allowed greater DNA recovery compared to the use of either 0.1 or 1.4 mm beads, irrespective of the tube supplier.These outcomes may help to improve commercial products in DNA/RNA extraction kits, besides raising awareness about the optimal choice of additives, offering opportunities for acquiring a better understanding of topics such as vertical microbial characterisation and environmental DNA recovery in low biomass samples.

scholarly journals The Impact of Land use on Bacterial Community Composition and Physicochemical Properties of Soil

2016 ◽  
Vol 13 (4) ◽  
pp. 2167-2176
Author(s):  
Ghobad Jalali ◽  
Amir Lakzian ◽  
Alireza Astaraei ◽  
Aliakbar Haddad- Mashadrizeh ◽  
Mehdi Azadvar ◽  
...  
Keyword(s):  
Land Use ◽  
Bacterial Community ◽  
Physicochemical Properties ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
The Impact ◽  
Properties Of Soil

Bacterial community composition in an Arctic phytoplankton mesocosm bloom: the impact of silicate and glucose

Polar Biology ◽  
2010 ◽  
Vol 33 (11) ◽  
pp. 1557-1565 ◽  
Author(s):  
Birte Töpper ◽  
Aud Larsen ◽  
Tron Frede Thingstad ◽  
Runar Thyrhaug ◽  
Ruth-Anne Sandaa
Keyword(s):  
Bacterial Community ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
The Impact
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