Construction of NotI restriction maps of human chromosome 21q and 11q and its application to cancer research.

Misao Ohki

doi:10.2198/sbk.43.57

Construction of NotI restriction maps of human chromosome 21q and 11q and its application to cancer research.

SEIBUTSU BUTSURI KAGAKU ◽

10.2198/sbk.43.57 ◽

1999 ◽

Vol 43 (2) ◽

pp. 57-61

Author(s):

Misao Ohki

Keyword(s):

Cancer Research ◽

Human Chromosome ◽

Restriction Maps ◽

Noti Restriction

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Distribution of interspersed repeats (Alu and Kpn) on NotI restriction fragments of human chromosome 21.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.89.3.1080 ◽

1992 ◽

Vol 89 (3) ◽

pp. 1080-1084 ◽

Cited By ~ 12

Author(s):

J. Sainz ◽

L. Pevny ◽

Y. Wu ◽

C. R. Cantor ◽

C. L. Smith

Keyword(s):

Human Chromosome ◽

Chromosome 21 ◽

Restriction Fragments ◽

Human Chromosome 21 ◽

Interspersed Repeats ◽

Noti Restriction

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On an Application of the Metallurgical Stage to Chromosome Morphology

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060155 ◽

1967 ◽

Vol 25 ◽

pp. 28-29

Author(s):

Godfrey C. Hoskins

Keyword(s):

Human Chromosome ◽

Electron Micrographs ◽

Chromosome Morphology ◽

Electron Optics ◽

Electron Micrograph ◽

Photographic Evidence ◽

Sophisticated Equipment ◽

Periodic Arrangement

The first serious electron microscooic studies of chromosomes accompanied by pictures were by I. Elvers in 1941 and 1943. His prodigious study, from the manufacture of micronets to the development of procedures for interpreting electron micrographs has gone all but unnoticed. The application of todays sophisticated equipment confirms many of the findings he gleaned from interpretation of images distorted by the electron optics of that time. In his figure 18 he notes periodic arrangement of pepsin sensitive “prickles” now called secondary fibers. In his figure 66 precise regularity of arrangement of these fibers can be seen. In his figure 22 he reproduces Siegbahn's first stereoscopic electron micrograph of chromosomes.The two stereoscopic pairs of electron micrographs of a human chromosome presented here were taken with a metallurgical stage on a Phillips EM200. These views are interpreted as providing photographic evidence that primary fibers (1°F) about 1,200Å thick are surrounded by secondary fibers (2°F) arranged in regular intervals of about 2,800Å in this metanhase human chromosome. At the telomere the primary fibers bend back on themselves and entwine through the center of each of each chromatid. The secondary fibers are seen to continue to surround primary fibers at telomeres. Thus at telomeres, secondary fibers present a surface not unlike that of the side of the chromosome, and no more susceptible to the addition of broken elements from other chromosomes.

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