scholarly journals The Gaschromatographic Determination of Fatty Acids. Composition of Serum Cholesterol Esters Separated by Thin Layer Chromatography

1966 ◽  
Vol 11 (4) ◽  
pp. 335-343
Author(s):  
Kazuro KOTODA ◽  
Nobuo OKAZAKI ◽  
Misako MURAKOSHI ◽  
Eiji ARAKI
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Serum Cholesterol ◽  
Cholesterol Esters ◽  
Fatty Acids Composition ◽  
Layer Chromatography

Fluorometric Determination of Plasma 11-Hydroxycorticosteroids. II. Studies on the Specificity of the Method

1973 ◽  
Vol 19 (7) ◽  
pp. 718-724 ◽  
Author(s):  
Luis E Mejer ◽  
Roberta C Blanchard
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Total Cholesterol ◽  
Thin Layer Chromatography ◽  
Plasma Cortisol ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Main Component ◽  
Layer Chromatography

Abstract We have investigated the specificity of the fluorometric method we proposed [Clin. Chem. 19, 710 (1973)] for plasma or serum 11-hydroxycorticosteroid determinations. The principal specific fluorogenic contaminants in the cortisol-containing extract of plasma, as detected by thin-layer chromatography and chemically, were triglycerides and total cholesterol. These contaminants contributed an average of 1.6 µg/dl to the total normal cortisol value. Fatty acids were also found, but did not fluoresce. Nonspecific serum fluorogens were quantitated at 1.3 ± 1.2 µg/dl. Cortisol, corticosterone, and fluorogenic contaminants represented an average of 62.0, 29.1, and 9.9%, respectively, of the total fluorometric plasma cortisol value obtained (expressed in terms of cortisol standard). A ratio of 8.3 ± 2.4 was found for cortisol/corticosterone when each component was determined in terms of its respective standard. Fluorescence scans of the plasma cortisol extract indicated cortisol to be the main component present, accompanied by minor fluorescent contaminants.

scholarly journals Development of a simple, low-cost and rapid thin-layer chromatography method for the determination of individual volatile fatty acids

2019 ◽  
Vol 11 (14) ◽  
pp. 1891-1897 ◽  
Author(s):  
Fabien Robert-Peillard ◽  
Elodie Mattio ◽  
Aïnhoa Komino ◽  
Jean-Luc Boudenne ◽  
Bruno Coulomb
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Volatile Fatty Acids ◽  
Low Cost ◽  
Chromatography Method ◽  
Thin Layer Chromatography Method ◽  
Layer Chromatography

In this paper, a new thin-layer chromatography (TLC) method for the determination of individual volatile fatty acids (VFAs) is presented.

scholarly journals Determination of higher fatty acids in phospholipid subfractions of human platelets using thin-layer chromatography and gas chromatography.

1985 ◽  
Vol 34 (11) ◽  
pp. 707-711 ◽  
Author(s):  
Akio OZAWA ◽  
Hiroe JINBO ◽  
Harumi TAKAHASHI ◽  
Takao FUJITA ◽  
Aizan HIRAI ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Platelets ◽  
Layer Chromatography ◽  
Higher Fatty Acids

Determination of Polysorbates in Foods by Colorimetry with Confirmation by Infrared Spectrophotometry, Thin-Layer Chromatography, and Gas Chromatography

1989 ◽  
Vol 72 (1) ◽  
pp. 27-29
Author(s):  
Haruyasu Kato ◽  
Yuji Nagai ◽  
Katsuhiko Yamamoto ◽  
Yoshio Sakabe
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methylene Chloride ◽  
Processed Foods ◽  
Infrared Spectrophotometry ◽  
Coefficients Of Variation ◽  
Layer Chromatography

Abstract A method is presented for the detection of polysorbates (PSs) in 8 kinds of processed foods by colorimetric and thin-layer chromatographic (TLC) techniques. The PSs are extracted from processed foods with a mixture of methylene chloride and ethanol by using an Extrelut column. The extract is further purified by using a silica gel column. The PS extract is complexed with cobalt-thiocyanate (Cothiocyanate) reagent and is determined spectrophotometrically at 620 nm. The recoveries and coefficients of variation for 8 kinds of processed foods fortified with 0.1% PS 80 were 67.9-94.6% and 4.0- 11.3%, respectively. The detection limit of TLC corresponded to 50 mg PS 80/kg. PS identity was confirmed by infrared spectrophotometry of PS extract, and gas chromatography of fatty acids and thin layer chromatography of POE-sorbitan residues after saponification.

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