scholarly journals Development of diced electrophoresis gel assay for screening disease-related enzymatic activities

2021 ◽  
Vol 65 (2) ◽  
pp. 29-33
Author(s):  
Toru Komatsu
Keyword(s):  
Enzymatic Activities

Effects of Liming and Fertilization on the Dehydrogenase and Catalase Activities

2019 ◽  
Vol 70 (10) ◽  
pp. 3464-3468
Author(s):  
Alina Dora Samuel ◽  
Simona Bungau ◽  
Ilona Katalin Fodor ◽  
Delia Mirela Tit ◽  
Cristian Felix Blidar ◽  
...  
Keyword(s):  
Soil Quality ◽  
Catalase Activity ◽  
Low Dose ◽  
Farmyard Manure ◽  
Enzymatic Activities ◽  
Mineral Fertilizers ◽  
Mineral Fertilization ◽  
Favorable Conditions ◽  
Npk Fertilization

In this paper we provide new data about the soil enzyme activity as a biological process, which is an indicator for impacts of factorial combinations of lime and fertilizers applications. Five plots divided into fifteen subplots were sampled for determination of the enzymatic indicators of soil quality, based on the actual and potential dehydrogenase and catalase activities. The research revealed that limed soil samples, in comparison with unlimed ones, resulted in significantly higher soil enzymatic activities (p[0.05) in the upper (0-20 cm), while in the deeper (20-40 cm) layer, only catalase activity was significantly higher (at least at p[0.02). Mineral fertilization, in comparison with its farmyard manuring, led to an insignificant increase in each of the three enzymatic activities determined, excepting catalase activity which was significantly higher (0.05]p]0.02) in the 0-20 cm layer. Based on the absolute values of the enzymatic activities, the enzymatic indicators of soil quality (EISQ) were calculated. The mineral NPK-fertilization and low dose of lime in the 0-20 cm layer, and mineral NP-fertilization and low dose of lime in the 20-40 cm layer proved to be the best variants of fertilization. The enzymatic indicators of soil quality in these variants reached the highest values: EISQ=0.821 and EISQ=0.889, respectively, indicating the presence of high enzymatic activities. It should be emphasized that a balanced application of lime, mineral fertilizers and farmyard manure leads to the formation of favorable conditions for the development of microorganisms, growth of plants and for an intense and lasting enzymatic activity.

Effects of Long Term Application of Organic and Mineral Fertilizers on Soil Enzymes

2018 ◽  
Vol 69 (10) ◽  
pp. 2608-1612 ◽  
Author(s):  
Alina Dora Samuel ◽  
Simona Bungau ◽  
Delia Mirela Tit ◽  
Carmen Elena Melinte (Frunzulica) ◽  
Lavinia Purza ◽  
...  
Keyword(s):  
Biological Activity ◽  
Soil Quality ◽  
Soil Enzymes ◽  
Farmyard Manure ◽  
Enzymatic Activities ◽  
Mineral Fertilizers ◽  
Soil Biology ◽  
Soil Biological Activity ◽  
Organic And Mineral Fertilizers

Long term productivity and conservation of soils is critical for sustaining agricultural ecosystems. The specific objective of the work reported was to determine the effects of long term application of organic and mineral fertilizers on soil enzyme activity as an index of soil biology and biochemistry. Three key soil enzymes involved in intracellular metabolism of microorganisms and two soil enzymes involved in phosphorus metabolism were selected. Actual and potential dehydrogenase, catalase, acid and alkaline phosphatase activities were determined in the 0-20 cm layer of an eroded soil submitted to a complex fertilization experiment. Results showed that addition of mineral fertilizers to organic (green manure and farmyard manure) fertilizers led to a significant increase in each activity because of increased plant biomass production which upon incorporation stimulates soil biological activity. The enzymatic indicators of soil quality calculated from the values of enzymatic activities depending on the kind of fertilizers showed that by the determination of enzymatic activities valuable information can be obtained regarding fertility status of soils. A weak positive correlation between enzymatic indicators of soil quality and maize yield was established. The yield data demonstrate the superiority of farmyard manure which provided greater stability in crop production. Substantial improvement in soil biological activity due to application of organic fertilizers with mineral fertilizers contribute in maintaining the productivity and soil health.

Structure and Enzymatic Activities of Human Serum Albumin

2015 ◽  
Vol 21 (14) ◽  
pp. 1831-1836 ◽  
Author(s):  
Yu Wang ◽  
Shihua Wang ◽  
Mingdong Huang
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Enzymatic Activities

Profiling of Enzymatic Activities Using Peptide Arrays

2011 ◽  
Vol 8 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Alexandra Thiele ◽  
Stephanie Posel ◽  
Michael Spinka ◽  
Johannes Zerweck ◽  
Ulf Reimer ◽  
...  
Keyword(s):  
Enzymatic Activities ◽  
Peptide Arrays

New Approaches for the Synthesis of Fused Thiophene, Pyrazole , Pyran and Pyridine Derivatives with Anti-Proliferative Together with c-Met Kinase and Prostate Cancer Cell Inhibitions

2020 ◽  
Vol 20 ◽  
Author(s):  
Rafat M. Mohareb ◽  
Yara R. Milad ◽  
Reem A. El-Ansary
Keyword(s):  
Prostate Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Aromatic Aldehydes ◽  
Cancer Cell Lines ◽  
Enzymatic Activities ◽  
Pyridine Derivatives ◽  
Wide Range ◽  
Target Molecules

Background:: Recently multi-component reactions producing pyran and pyridine derivatives acquired a special attention due to their wide range of pharmacological activities especially the therapeutic activities. Through the market it was found that many pharmacological drugs containing the pyran and pyridine nucleus were known. Objective:: We are aiming in this work to synthesize target molecules not only possess anti-tumor activities but also kinase inhibitors. The target molecules were obtained starting from cyclohexan-1,3-dione followed by its heterocyclization reactions to produce anticancer target molecules. Methods:: This work demonstrated multi-component reactions of cyclohexan-1,3-dione with aromatic aldehydes and diethylmalonate using triethylamine as a catalyst to give the 7,8-dihydro-4H-chromen-5(6H)-one derivatives 4a-c. The reaction of compounds 4a-c with either of hydrazine hydrate of phenylhydrazine gave the chromeno[2,3-c]pyrazole derivatives 5a-f, respectively. In addition, further heterocyclization reactions were adopted to give the chromeno[3,2-d]isoxazole, chromene-3-carboxamide derivatives. Moreover, the multi-component reaction of cyclohexan-1,3-dione (1) with either of aromatic aldehydes and diethylmalonate using a catalytic amount of ammonium acetate gave the 1,4,5,6,7,8-hexahydroquinoline derivatives 13a-c. The anti-proliferative activities of the synthesized compounds toward the six cancer cell lines namely A549, H460, HT-29, MKN-45, U87MG, and SMMC-7721 were studied. In addition the c-Met enzymatic activities and inhibition toward the prostate cancer cell PC-3 were measured. Results:: Anti-proliferative evaluations, c-Met enzymatic activities and inhibition toward the prostate cancer cell PC-3 were measured and the results obtained in most cases, indicated that the presence of electronegative Cl group through the molecule favour the inhibitions. Conclusion:: The compounds with high anti-proliferative activity towards the cancer cell lines were 4a, 4b, 6d, 6e, 6f, 10e, 10f, 12c, 14e, 14f, 15c, 16d, 16e, 16f, 19c and 20c. Compounds 4b, 6c, 6d, 8b, 10c, 10d, 12b, 13b, 14c, 14d, 15b, 16c, 16d, 17b, 17c, 19b, 20b and 20c exhibited high potency against c-Met kinase and compounds 4a, 4b, 6b, 6c, 6d, 6f, 8b, 8c, 10c, 10d, 10e, 12b, 12c, 13a, 13b, 13c, 14c, 14d, 14e, 14f, 15b, 15c, 16b, 16c, 16d, 17b, 17c, 19c, 19d, 20a, 20b and 20c displayed high inhibitions toward PC-3 cell line.

scholarly journals Effect of ecological and conventional managements on soil enzymatic activities in coffee agroecosystems

2018 ◽  
Vol 48 (4) ◽  
pp. 420-428
Author(s):  
Johana Juliet Caballero Vanegas ◽  
Karen Bibiana Mejía Zambrano ◽  
Lizeth Manuela Avellaneda-Torres
Keyword(s):  
Sustainable Management ◽  
Climatic Variability ◽  
Agricultural Practices ◽  
Enzymatic Activities ◽  
Acid And Alkaline Phosphatase ◽  
Dry Seasons ◽  
Synthetic Fertilizers ◽  
Coffee Agroecosystems ◽  
Alkaline And Acid Phosphatase

ABSTRACT Understanding the impacts of agricultural practices on soil quality indicators, such as enzymatic activities, is of great importance, in order to advance in their diagnosis and sustainable management. This study aimed to evaluate the effect of ecological and conventional agricultural managements on enzymatic activities of a soil under coffee agroecosystems. The enzymatic activities were associated with the biogeochemical cycles of nitrogen (urease and protease), phosphorus (acid and alkaline phosphatase) and carbon (β-glucosidase), during the rainy and dry seasons. Physical-chemical soil proprieties were also assessed and related to resilience scores linked to the climatic variability reported for the areas under study. The activities of urease, alkaline and acid phosphatase and ß-glucosidase were statistically higher in ecological agroecosystems than in conventional ones. This may be attributed to the greater application of organic waste in the ecological environment, as well as to the absence of pesticides and synthetic fertilizers, which allow better conditions for the microbial activity. The resilience scores to the climate variability that showed the highest correlations with the assessed enzymatic activities were: the farmers' knowledge on soil microorganisms, non-use of pesticides and synthetic fertilizers and non-dependence on external supplies. It was concluded that the enzymatic activities are modified by the management systems, being specifically favored by the ecological management. This agroecosystem, in the long term, ensures an efficient use of the soil resources, with a lower degradation and contamination.

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