scholarly journals Conjugation of Silver Nanoparticles with De Novo Engineered Cationic Antimicrobial Peptides: An Exploratory Proposal (Preprint)

10.2196/28307 ◽  
2021 ◽  
Author(s):  
Alvin Hu
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Peptides ◽  
De Novo ◽  
Cationic Antimicrobial Peptides

Conjugation of Silver Nanoparticles with De Novo Engineered Cationic Antimicrobial Peptides: Proposal for Study (Preprint)

2021 ◽  
Author(s):  
Alvin Hu
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Antimicrobial Peptides ◽  
Inhibitory Concentration ◽  
De Novo ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Cationic Antimicrobial Peptides ◽  
Drug Resistant Bacteria

BACKGROUND Cationic antimicrobial peptides have broad antimicrobial activity and provide a novel way of targeting multi drug resistant bacteria in an era of increasing antimicrobial resistance. Current developments show positive prospects for both antimicrobial peptides and silver nanoparticles individually. OBJECTIVE The primary objective is to propose another method of enhancing antimicrobial activity by conjugating silver nanoparticles with cationic antimicrobial peptides for a subsequent preliminary assessment on studying the minimum inhibitory concentration of multi drug resistant bacteria. The secondary objective would be to evaluate the safety of the conjugated compound to assess viability for in vivo use. METHODS The proposition is planned for approximately 3 overarching stages. Firstly, I propose synthesis of wlbu2c, a modified version of antimicrobial peptide wlbu2 with an added cysteine group, using standard Fmoc procedure. This will subsequently be attempted to stably conjugate with silver nanoparticles ideally through photochemical means. Secondly, the conjugate wlbu2c-AgNP will be tested for antimicrobial activity following Clinical & Laboratory Standards Institute Manual on standard minimum inhibitory concentration testing. If all of the above is completed the experiment can progress to the assessment of cytotoxicity using cell lysis assays. RESULTS I-TASSER simulation revealed that our modified peptide wlbu2c has similar secondary structure to original wlbu2 peptide. No other results have been obtained at this time other than aforementioned theoretical propositions. CONCLUSIONS The addition of silver nanoparticles to already developing de novo engineered antimicrobial peptides provide a second degree of freedom toward the development of potent antimicrobials. Future prospects include emergency last line therapy, treatment for current difficult to eradicate bacterial colonization such as in cystic fibrosis, implantable medical devices, cancer and immunotherapy. This proposal is intended to be provided to the public as I do not anticipate funding at this time.

Cationic Antimicrobial Peptides for Tuberculosis: A Mini-Review

2019 ◽  
Vol 20 (9) ◽  
pp. 885-892
Author(s):  
Sara Silva ◽  
Nuno Vale
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Amino Acid ◽  
Antimicrobial Peptides ◽  
Resistance Mechanisms ◽  
Therapeutic Agents ◽  
Pharmacological Properties ◽  
Therapeutic Activity ◽  
Alternative Strategies ◽  
Cationic Antimicrobial Peptides ◽  
Specific Amino Acid

Cationic antimicrobial peptides (CAMPs) can be considered as new potential therapeutic agents for Tuberculosis treatment with a specific amino acid sequence. New studies can be developed in the future to improve the pharmacological properties of CAMPs and also understand possible resistance mechanisms. This review discusses the principal properties of natural and/or synthetic CAMPs, and how these new peptides have a significant specificity for Mycobacterium tuberculosis. Also, we propose some alternative strategies to enhance the therapeutic activity of these CAMPs that include coadministration with nanoparticles and/or classic drugs.

Membrane targeting cationic antimicrobial peptides

2019 ◽  
Vol 537 ◽  
pp. 163-185 ◽  
Author(s):  
Daniela Ciumac ◽  
Haoning Gong ◽  
Xuzhi Hu ◽  
Jian Ren Lu
Keyword(s):  
Antimicrobial Peptides ◽  
Membrane Targeting ◽  
Cationic Antimicrobial Peptides

Cationic Antimicrobial Peptides in Penaeid Shrimp

2011 ◽  
Vol 13 (4) ◽  
pp. 639-657 ◽  
Author(s):  
Anchalee Tassanakajon ◽  
Piti Amparyup ◽  
Kunlaya Somboonwiwat ◽  
Premruethai Supungul
Keyword(s):  
Antimicrobial Peptides ◽  
Penaeid Shrimp ◽  
Cationic Antimicrobial Peptides

scholarly journals Generation of novel cationic antimicrobial peptides from natural non-antimicrobial sequences by acid-amide substitution

2011 ◽  
Vol 10 (1) ◽  
pp. 11 ◽  
Author(s):  
Satoshi Ueno ◽  
Masaomi Minaba ◽  
Yuji Nishiuchi ◽  
Misako Taichi ◽  
Yasushi Tamada ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Acid Amide ◽  
Cationic Antimicrobial Peptides
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