scholarly journals Subclinical laminitis and its association with pO2 and faecal alterations: Isikli, Aydin experience

2015 ◽  
pp. 4534-4543
Author(s):  
Ibrahim Akin ◽  
Deniz Alic Ural ◽  
Mehmet Gultekin ◽  
Kerem Ural
Keyword(s):  
Dairy Cows ◽  
Healthy Controls ◽  
Blood Gas ◽  
Daily Ration ◽  
Sample Collection ◽  
Blood Samples ◽  
Blood Ph ◽  
Para Determinar ◽  
Los Grupos ◽  
Faecal Ph

ABSTRACTObjective. The aim of this field trial was to investigate the relationships among subclinical laminitis, hematological, ruminal and faecal alterations. Materials and Methods. To this extent dairy cows presenting subclinical laminitis (n=11) and to those of other healthy cows without laminitis (n=10) were enrolled and assigned into two groups. All animals were receiving the same daily ration formulated to contain 47% cornsilage and 18% hay, mainly. Effects of subclinical laminitis challenges on measurements of feces, and blood samples, were investigated to determine which of these measurements may aid in the diagnosis. pH changes in ruminal fluid collected via rumenocentesis were measured. Besides the following parameters were also measured; blood pH, faecal pH and faecal scoring. Blinded investigators performed the sample collection. Results. No statistical differences between the groups were detected for blood gas values studied regarding pCO2, HCO3, BE, indeed mean that pO2 values decreased statistically (p<0.05) and faecal pH was significantly decreased (p<0.05) in cows with subclinical laminitis in contrast to healthy controls. Conclusions. pO2 values and faecal pH may be valuable as indirect indicators of subclinical laminitis in cattle.RESUMENObjetivos. El objetivo de esta prueba de campo fue investigar las relaciones entre la laminitis subclínicay alteraciones hematológicas, ruminales y fecales. Materiales y métodos. Las vacas lecheras que presentaron laminitis subclínica (n=11) y las vacas sanas sin laminitis (n=10) fueron reclutadas y asignadas en dos grupos. Todos los animales recibieron la misma ración diaria que contenía 47% de ensilaje de maíz y 18% de heno, principalmente. Los efectos de la laminitis subclínica sobre las mediciones de las heces y muestras de sangre, fueron investigados para determinar cuál de estas mediciones pueden ayudar en el diagnóstico. Se midieron los cambios de pH en el fluido ruminal recogido a través rumenocentesis. Además, también se midieron los siguientes parámetros; pH de la sangre, el pH fecal y la puntuación fecal. La toma de las muestras se realizó a doble ciego. Resultados. No se detectaron diferencias significativas entre los grupos para los valores de los gases sanguíneos estudiados en relación con la pCO2, HCO3, BE; lo que significa que los valores de pO2 disminuyeron estadísticamente (p<0.05) y que el pH fecal se redujo significativamente (p<0.05) en las vacas con laminitis subclínica; en contraste con los controles sanos. Conclusiones. Los valores de PO2 y pH fecal pueden ser valiosos como indicadores indirectos de la laminitis subclínica en el ganado.

Blood gas analyses, ruminal and blood pH, urine and faecal pH in dairy cows during subacute ruminal acidosis

2008 ◽  
Vol 18 (3) ◽  
pp. 229-232 ◽  
Author(s):  
M. Morgante ◽  
M. Gianesella ◽  
S. Casella ◽  
L. Ravarotto ◽  
C. Stelletta ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Blood Gas ◽  
Subacute Ruminal Acidosis ◽  
Ruminal Acidosis ◽  
Blood Ph ◽  
Blood Gas Analyses ◽  
Faecal Ph

scholarly journals Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement

2018 ◽  
Vol 5 (4) ◽  
pp. 103
Author(s):  
Marlene Sickinger ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend
Keyword(s):  
Substance P ◽  
Dairy Cows ◽  
Vasoactive Intestinal Polypeptide ◽  
Serum Levels ◽  
Common Disease ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Breed Difference ◽  
Abomasal Displacement

Abomasal displacement (AD) to the left is a common disease in high-yielding dairy cows after parturition. In view of the previously reported changes in tissue neuropeptide concentrations in cows with AD, the primary aim of this study was to evaluate the effect of AD and breed on serum neuropeptide concentrations. For this purpose, blood samples of 33 German Holstein (GH) cows with AD, 36 healthy controls (GH), and 32 healthy German Fleckvieh (GF) cows were collected, and concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin1β (IL-1β) were measured via commercially available ELISA kits. To examine the effect of AD, we compared GH cows with and without AD and observed no significant effects of AD on SP, VIP, or Il-1 β concentrations. To evaluate the effect of breed, we compared healthy GH with healthy GF cows and detected markedly higher VIP serum levels in the healthy GF cows (p < 0.01). No significant differences in SP or IL-1β were detected. According to our results, there seems to be no effect of AD on the serum concentrations of SP, VIP, or IL-1 β. In contrast, there seems to be a breed difference concerning serum VIP concentrations.

scholarly journals Intraocular pressure and its correlation with blood gas parameters in crested caracaras (Caracara plancus) anesthetized with isoflurane and sevoflurane

2008 ◽  
Vol 60 (6) ◽  
pp. 1461-1467
Author(s):  
A.P. Ribeiro ◽  
S.N. Vitaliano ◽  
R. Thiesen ◽  
A. Escobar ◽  
J.P. Duque Ortiz ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Intraocular Pressure ◽  
Brachial Artery ◽  
Carbon Dioxide Partial Pressure ◽  
Blood Gas ◽  
Blood Samples ◽  
Time Points ◽  
Blood Ph ◽  
Arterial Ph ◽  
Blood Gas Parameters

The intraocular pressure (IOP) and its correlations with arterial carbon dioxide partial pressure (PaCO2) and arterial pH were studied in five crested caracaras (Caracara plancus) anesthetized with isoflurane (ISO) and sevoflurane (SEV). Baseline IOP values were measured in both eyes (M0). Brachial artery was previously catheterized to obtain blood gas and cardiorespiratory analysis. Anesthesia was induced with 5% ISO and maintained with 2.5% for 40 minutes. IOP measurements and blood samples were evaluated in different moments until the end of the procedure. After recovering, a second anesthesia was induced with 6% SEV and maintained with 3.5%. Parameters were evaluated at the same time points of the previous procedure. IOP reduced significantly (P= 0.012) from M0 at all time points and no significative changes were observed between ISO and SEV anesthesias. Correlation between IOP and PaCO2 and between PIO and blood pH were found only for SEV. IOP and blood pH decreased in parallel with IOP, whereas values of PaCO2 increased in caracaras anesthetized with isoflurane and sevoflurane.

Normal arterial blood pH, oxygen, and carbon dioxide tensions in unanesthetized dogs

1972 ◽  
Vol 32 (1) ◽  
pp. 152-153 ◽  
Author(s):  
Eric O. Feigl ◽  
Louis G. D'Alecy
Keyword(s):  
Carbon Dioxide ◽  
Rectal Temperature ◽  
Femoral Artery ◽  
Glass Electrode ◽  
Blood Gas ◽  
Blood Samples ◽  
Arterial Blood ◽  
Blood Ph ◽  
Clark Electrode

Femoral artery blood samples from 30 unanesthetized unrestrained nonpanting dogs were analyzed. Average normal pH was 7.42 (sd 0.03), mean Po2 89.5 mm Hg (sd 4.4), and Pco2 36.8 mm Hg (sd 2.4). pH was determined with a glass electrode. Pco2 was measured using a Severinghaus electrode. Po2 was determined with a Clark-type polarographic polypropylene-covered electrode. Measurements were made at 39 C (normal dog rectal temperature). blood gas; Clark electrode; Severinghaus electrode

scholarly journals Effects of time and temperature on blood gas and electrolytes in equine venous blood

2020 ◽  
Vol 89 (3) ◽  
pp. 239-246
Author(s):  
José Dantas Ribeiro Filho ◽  
Paulo Vinícius de Morais Santos ◽  
Samuel Rodrigues Alves ◽  
Lorena Chaves Monteiro ◽  
Caio Monteiro Costa ◽  
...  
Keyword(s):  
Gradual Increase ◽  
Potassium Concentration ◽  
Venous Blood ◽  
Blood Gas Analysis ◽  
Gas Analysis ◽  
Blood Gas ◽  
Blood Samples ◽  
Blood Ph ◽  
Erythrocyte Metabolism

This study aimed to evaluate the viability time of horse venous blood samples kept at laboratory temperature (LT) and in water with ice (WI), to perform blood gas analysis. Eleven blood samples were collected in duplicates from 10 healthy horses. The samples were transported to the laboratory and subjected to one of the 24 h storage method. Each pair of syringes was distinctly kept at LT or submerged in WI. Blood gas tests were performed at times T0h, T1h, T2h, T3h, T4h, T5h, T6h, T8h, T10h, T12h and T24h after collection. Analyses of electrolytes were also performed from the same samples. A difference in blood pH was found between the treatments (P < 0.05). From T4h, pH decreased in samples kept at LT, but in WI, pH did not change. For partial pressure of carbon dioxide (pCO2), a difference between treatments (P < 0.05) was noted starting at T8h. In samples kept at LT, pCO2 increased; no changes occurred in samples stored in WI. There was a decrease in the base concentration beginning at T5h in samples kept at LT (P < 0.05), but no variation in samples kept in WI. These changes can be attributed to the erythrocyte metabolism, still active in vitro, which generates lactic acid from anaerobic glycolysis. The potassium concentration increased in samples kept in WI from T4h, with a gradual increase until T24h. Conservation of equine venous blood samples in WI is efficient in reducing cellular metabolism, thereby increasing the viability of samples for examination and interpretation of results.

scholarly journals Spontaneous alteration of blood pH by a bicarbonate buffer system during experimental hypercalcaemia in cows

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Younghye Ro ◽  
Woojae Choi ◽  
Leegon Hong ◽  
Eunkyung Kim ◽  
Eunhui Choe ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Dairy Cows ◽  
Calcium Concentration ◽  
Crossover Design ◽  
Buffer System ◽  
Blood Gas ◽  
Urine Calcium ◽  
Bicarbonate Buffer ◽  
Blood Ph ◽  
Blood And Urine Samples

Abstract Introduction Maintaining mineral homeostasis as well as the secretion and metabolism of mineralotropic hormones is important for healthy of periparturient dairy cows. To increase the activity of mineralotropic hormones, blood pH can be adjusted. The purpose of this study was to investigate changes in blood pH and the mechanism of action of this change in induced hypercalcaemic cows. Material and Methods Six non-lactating Holstein cows were used in a 2 × 2 crossover design. To induce hypercalcaemia, calcium borogluconate was administered subcutaneously to experimental cows and normal saline was administered subcutaneously to control cows. Blood and urine samples were collected serially after administration. Whole blood without any anticoagulant was processed with a portable blood gas analyser. Plasma concentration and urinary excretion of calcium were measured. Results In hypercalcaemic cows, both blood and urine calcium levels were significantly increased at 8 h compared to those at 0 h (P < 0.05), and a spontaneous increase in blood pH was also observed. The calcium concentration in plasma was highest at 2 h after administration (3.02 ± 0.27 mmol/L). The change in pH correlated with that in bicarbonate (r = 0.781, P < 0.001) rather than that in partial pressure of CO2 (r = 0.085, P = 0.424). Conclusion Hypercalcaemia induced a spontaneous change in blood pH through the bicarbonate buffer system and this system may be a maintainer of calcium homeostasis.

scholarly journals Association of intraoperative blood gas analysis with patient outcome after adult cardiac surgery

2019 ◽  
Author(s):  
Shaopeng Gang ◽  
Ling Jiang ◽  
Kaiyun Fang ◽  
Xiulun Liu ◽  
Daokang Xiang ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Venous Blood ◽  
Jugular Bulb ◽  
Blood Gas ◽  
Central Venous ◽  
Blood Samples ◽  
Central Venous Blood ◽  
Blood Ph ◽  
Blood Gas Parameters ◽  
Adult Cardiac Surgery

Abstract Background: The associations of the different blood gas parameters from different blood samples harvested at different stages during adult cardiac surgery with the postoperative outcomes are inadequately studied. Methods: Adult patients undergoing elective cardiac surgery with cardiopulmonary bypass (CPB) participated in this prospective observational study. Blood gas parameters from arterial, central venous, and jugular bulb venous blood samples harvested simultaneously at pre-determined time points (baseline with the patient awake, post-anesthesia induction but before CPB, during CPB at 30°C, during CPB at 37°C (rewarming), and at the end of surgery) were correlated with postoperative outcomes including the length of mechanical ventilation (LMV), intensive care unit stay (LICU), hospital stay (LOH), and major organ morbidity and mortality. Results: Data from 193 patients were analyzed. Multiple parameters of different blood harvested at different stages significantly correlated with one or more outcome measures based on univariate analysis (p < 0.05). However, only the jugular bulb venous blood pH and carbon dioxide tension and the central venous blood pH at the end of surgery (pHcv-end) were significantly correlated with LMV, LICU, and LOH (p < 0.05). A more alkaline blood correlated with more favorable outcomes. After adjusting for age, surgical time, and total intravenous volume administered, multivariate analysis showed that only pHcv-end remained independently associated with LMV and LICU (p < 0.05). Conclusion: More alkaline blood, especially the central venous blood at the end of surgery, is associated with more favorable outcomes after adult cardiac surgery. Trial registration ChiCTR-POC-17013942, Date of registration December 15, 2017.

scholarly journals Association of intraoperative blood gas analysis with patient outcome after adult cardiac surgery

2019 ◽  
Author(s):  
Shaopeng Gang ◽  
Ling Jiang ◽  
Kaiyun Fang ◽  
Xiulun Liu ◽  
Daokang Xiang ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Venous Blood ◽  
Postoperative Outcomes ◽  
Blood Gas ◽  
Central Venous ◽  
Blood Samples ◽  
Central Venous Blood ◽  
Blood Ph ◽  
Blood Gas Parameters ◽  
Adult Cardiac Surgery

Abstract Background: The associations of the different blood gas parameters from different blood samples harvested at different stages during adult cardiac surgery with the postoperative outcomes are inadequately studied. Methods: Adult patients undergoing elective cardiac surgery with cardiopulmonary bypass (CPB) participated in this prospective observational study. Blood gas parameters from arterial, central venous, and jugular venous blood samples harvested simultaneously at pre-determined time points (baseline with the patient awake, post-anesthesia induction but before CPB, during CPB at 30°C, during CPB at 37°C (rewarming), and at the end of surgery) were correlated with postoperative outcomes including the length of mechanical ventilation (LMV), intensive care unit stay (LICU), hospital stay (LOH), and major organ morbidity and mortality. Results: Data from 193 patients were analyzed. Multiple parameters of different blood harvested at different stages significantly correlated with one or more outcome measures based on univariate analysis (p < 0.05). However, only the jugular venous blood pH and carbon dioxide tension and the central venous blood pH at the end of surgery (pHcv-end) were significantly correlated with LMV, LICU, and LOH (p < 0.05). A more alkaline blood correlated with more favorable outcomes. After adjusting for age, surgical time, and total intravenous volume administered, multivariate analysis showed that only pHcv-end remained independently associated with LMV and LICU (p < 0.05). Conclusion: More alkaline blood, especially the central venous blood at the end of surgery, is associated with more favorable outcomes after adult cardiac surgery. Trial registration ChiCTR-POC-17013942, Date of registration December 15, 2017.

Los Criterios de Vinculación y la Responsabilidad Solidaria en los Grupos de Empresas. A Propósito de una Casación en Materia Laboral

2020 ◽  
Vol 2 (1) ◽  
pp. 125-134
Author(s):  
Erika Valdivieso López ◽  
Milagros Olivos Celis
Keyword(s):  
Para Determinar ◽  
Los Grupos

En observancia de los artículo 5 y 7 del Reglamento de Propiedad indirecta, vinculación y grupos económicos que defienden la vinculación económica y en virtud del principio de la primacía de la realidad queda debidamente probado en autos la vinculación económica y empresarial existente entre la recurrente y (…) al conformar ambas empresas un mismo grupo económico y empresaria, y ello es así no sólo por la participaciones de acciones en ambas empresas de una misma persona, sino por la relación familiar de los accionistas que pertenecen al mismo grupo empresarial, y que ambas empresas funcionan en el mismo local. El presente artículo comenta la Casación Laboral N° 3733-2009-Lima de la Sala de Derecho Constitucional y Social Permanente de la Corte Suprema (publicado en el Diario oficial El Peruano el 1 de diciembre del 2011), mediante la cual se pretenden establecer los criterios para determinar cuándo existe vinculación económica entre empresas que acarree como consecuencia la obligación solidaria en materia laboral, alegando el principio de primacía de la realidad. Tópicos afrontados de forma insuficiente, pues el resultado es que en dicha resolución- debido a su escasa motivación- plantea más dudas que certezas respecto a este tema discutido, pero al parecer aun no definido.

scholarly journals Blood transcriptome analysis of patients with uncomplicated bacterial infection and sepsis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Velma Herwanto ◽  
Benjamin Tang ◽  
Ya Wang ◽  
Maryam Shojaei ◽  
Marek Nalos ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Healthy Controls ◽  
Beta Globin ◽  
Transcriptome Data ◽  
Valuable Resource ◽  
Globin Mrna ◽  
Blood Samples ◽  
Data Description ◽  
Pathway Activation ◽  
Blood Transcriptome

Abstract Objectives Hospitalized patients who presented within the last 24 h with a bacterial infection were recruited. Participants were assigned into sepsis and uncomplicated infection groups. In addition, healthy volunteers were recruited as controls. RNA was prepared from whole blood, depleted from beta-globin mRNA and sequenced. This dataset represents a highly valuable resource to better understand the biology of sepsis and to identify biomarkers for severe sepsis in humans. Data description The data presented here consists of raw and processed transcriptome data obtained by next generation RNA sequencing from 105 peripheral blood samples from patients with uncomplicated infections, patients who developed sepsis, septic shock patients, and healthy controls. It is provided as raw sequenced reads and as normalized log2 transformed relative expression levels. This data will allow performing detailed analyses of gene expression changes between uncomplicated infections and sepsis patients, such as identification of differentially expressed genes, co-regulated modules as well as pathway activation studies.

Sign in / Sign up

Export Citation Format

Share Document