scholarly journals Lectinhistochemical staining of granuloma induced by bacillus Calmette-Guerin in Piaractus mesopotamicus

2013 ◽  
pp. 3753-3758
Author(s):  
Wilson G. Manrique ◽  
Gustavo S. Claudiano ◽  
Mayra AP. Figueiredo ◽  
Thalita R. Petrillo ◽  
Paulo F. Marcusso ◽  
...  
Keyword(s):  
Wheat Germ ◽  
Close Contact ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Control Group ◽  
Bacillus Calmette Guerin ◽  
Epithelioid Cells ◽  
Dolichos Biflorus ◽  
Piaractus Mesopotamicus ◽  
Helix Pomatia Agglutinin

ABSTRACTObjetive. This study was conducted to evaluate, by means of lectinhistochemistry (LHC), the expression of carbohydrates in granulomas induced by the bacillus Calmette-Guerin (BCG) in muscle tissue of Piaractus mesopotamicus after 33 days. Material and methods. Histological sections with 3 μm thick were incubated with the following lectins :WGA (Wheat germ agglutinin), DBA (Dolichos biflorus agglutinin) and HPA (Helix pomatia agglutinin), and the results were evaluated by light microscopy. Results. Acid fast bacilli were stained by Ziehl Neelsen (ZN) and strong labeled by WGA in the cytoplasm of macrophages. Labeling with DBA was intense in fibroblasts and weak in macrophages. On the other hand, HPA binding was stronger in macrophages, especially in those that were in close contact with epithelioid cells, without evidence of binding to fibroblasts. The epithelioid cells were not labeled by the used lectins, but they were identified by Hematoxilin-Eosin (HE). The lectins labeled specific type saccharides in glycoproteins, as N-acetylglucosamine present in bacilli and macrophages, as well as N-acetyl-galactosamine in macrophages. The control group showed no inflammation or lectin binding. Conclusions. This technique may be useful in identifying receptors for WGA, DBA and the HPA lectins in epithelioid granuloma induced by BCG in P. mesopotamicus

scholarly journals Lectin-binding properties of human breast cancer cell lines and human milk with particular reference to Helix pomatia agglutinin.

1995 ◽  
Vol 43 (3) ◽  
pp. 275-281 ◽  
Author(s):  
U Schumacher ◽  
E Adam ◽  
S A Brooks ◽  
A J Leathem
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Binding Sites ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Membrane Glycoproteins ◽  
Tissue Sections ◽  
Helix Pomatia Agglutinin

Several studies have shown binding of a variety of lectins to breast cancer cells in tissue sections. In particular, binding of the lectin from the Roman snail, Helix pomatia agglutinin (HPA), to breast cancer cells is linked with a poor prognosis. The molecular basis for lectin binding to metastatic breast cancers is not known. To elucidate this in a model system, lectin-binding patterns of seven human breast cancer cell lines were investigated, their cell membranes were isolated, and HPA binding was assessed. In addition, the influence of fixation and processing on lectin-binding sites was also investigated. Binding of lectins to the tumor cells was very heterogeneous between and within the different cell lines and was influenced by fixation and processing. However, some cell lines showed HPA-binding sites both in vivo and in tissue sections. Analysis of the isolated cell membrane glycoproteins from these cell lines on Western blots revealed that HPA can bind to several membrane glycoproteins. In contrast, human milk shows only one major milk glycoprotein that is HPA-positive. Therefore, a switch in glycosylation appears to be taking place during the transformation to a metastatic phenotype.

Glycocalyx characterisation and glycoprotein expression of Sus domesticus epididymal sperm surface samples

2012 ◽  
Vol 24 (4) ◽  
pp. 619 ◽  
Author(s):  
Anna Fàbrega ◽  
Marta Puigmulé ◽  
Jean-Louis Dacheux ◽  
Sergi Bonet ◽  
Elisabeth Pinart
Keyword(s):  
Lectin Binding ◽  
Helix Pomatia ◽  
Surface Proteins ◽  
Surface Glycoprotein ◽  
Ulex Europaeus ◽  
Sperm Surface ◽  
Epididymal Maturation ◽  
Helix Pomatia Agglutinin ◽  
Ulex Europaeus Agglutinin I ◽  
Principal Piece

The sperm surface is covered with a dense coating of carbohydrate-rich molecules. Many of these molecules are involved in the acquisition of fertilising ability. In the present study, eight lectins (i.e. Arachis hypogae (peanut) agglutinin (PNA), Lens culimaris (lentil) agglutinin-A (LCA), Pisum sativum (pea) agglutin (PSA), Triticum vulgari (wheat) germ agglutinin (WGA), Helix pomatia agglutinin (HPA), Phaseolus vulgaris (red kidney bean) leucoagglutinin (PHA-L), Glycine max (soybean) agglutinin (SBA) and Ulex europaeus agglutinin I (UEA-I)) were investigated to identify changes in the nature and localisation of glycoproteins in boar spermatozoa migrating along the epididymal duct. Complementary procedures included measurement of global lectin binding over the surface of the viable sperm population by flow cytometry, analysis of lectin localisation on the membrane of individual spermatozoa using fluorescence microscopy and the electrophoretic characterisation of the major sperm surface glycoprotein receptors involved in lectin binding. A significant increase was found in sperm galactose, glucose/mannose and N-acetyl-d-glucosamine residues distally in the epididymis. Moreover, the sperm head, cytoplasmic droplet and midpiece were recognised by most of the lectins tested, whereas only HPA and WGA bound to the principal piece and end piece of the sperm tail. Fourteen sperm surface proteins were observed with different patterns of lectin expression between epididymal regions. The sperm glycocalyx modifications observed in the present study provide an insight into the molecular modifications associated with epididymal maturation, which may be correlated with the degree of maturation of ejaculated spermatozoa.

Effects of an Oxidative Agent and Lectins on the Binding Inhibition of Recombinant Hepatitis A Virus Proteins to Oyster Digestive Tissues

2011 ◽  
Vol 74 (1) ◽  
pp. 157-160 ◽  
Author(s):  
SANG-MU KO ◽  
JONG-OH KIM ◽  
MYUNG-JOO OH ◽  
DUWOON KIM
Keyword(s):  
Binding Sites ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Helix Pomatia ◽  
Control Group ◽  
Carbohydrate Binding ◽  
Dolichos Biflorus ◽  
Ulex Europaeus ◽  
Binding Inhibition ◽  
Viral Contaminants

While the exact mechanism of hepatitis A virus (HAV) accumulation remains unclear, it has been demonstrated that viruses related to shellfish-borne gastroenteritis can bind to carbohydrates of oyster tissues. We investigated carbohydrate-binding sites to determine if they were related to the binding of HAV to carbohydrate moieties on oyster digestive tissues (DTs) using recombinant HAV proteins (rHAVPs). In addition, we evaluated lectins to determine if they influenced the inhibition of binding of rHAVPs to carbohydrates present in DT. DT that was treated with 0.5% potassium periodate allowed only 23% ± 3.6% and 33% ± 7.8% binding of VP1-P2A and VP1 rHAVPs, respectively, when compared with a control group (100%) treated with distilled water, indicating that carbohydrate-binding sites are strongly related to the binding of HAV. Soybean agglutinin (SBA) led to the greatest decrease in the binding affinity among six lectins (Helix pomatia, Dolichos biflorus, Ulex europaeus, SBA, Triticum vulgaris, and Arachis hypogaea) tested for inhibition of the binding of rHAVPs to DT, indicating that exposing the virus-contaminated DT to SBA might have the potential to depurate viral contaminants found in shellfish food products by high-affinity binding between SBA and rHAVPs, thus improving food safety.

scholarly journals Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer

2001 ◽  
Vol 95 (2) ◽  
pp. 79-85 ◽  
Author(s):  
Miriam V. Dwek ◽  
Heidi A. Ross ◽  
Andrew J. Streets ◽  
Susan A. Brooks ◽  
Elizabeth Adam ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Helix Pomatia Agglutinin ◽  
Aggressive Breast Cancer

scholarly journals Lectins for electron microscopic distinction of eosinophils from other blood cells.

1989 ◽  
Vol 37 (5) ◽  
pp. 743-749 ◽  
Author(s):  
M Eguchi ◽  
T Ozawa ◽  
J Suda ◽  
K Sugita ◽  
T Furukawa
Keyword(s):  
Blood Cells ◽  
Colloidal Gold ◽  
Helix Pomatia ◽  
Electron Microscopic Observation ◽  
Soybean Agglutinin ◽  
Electron Microscopic ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin ◽  
Griffonia Simplicifolia ◽  
Helix Pomatia Agglutinin

Colloidal gold-labeled soybean agglutinin (SBA), Helix pomatia agglutinin (HPA), Dolichos biflorus agglutinin (DBA), and Griffonia simplicifolia lectin (GS-1) were used for electron microscopic observation of blood cells. Colloidal gold-labeled SBA, HPA, and DBA showed marked deposition on eosinophil granules at all stages of maturation. Gold particles were not deposited on basophils, neutrophils, monocytes, lymphocytes, or other blood cells. Only a few colloidal gold-labeled GS-1 were deposited on eosinophil granules. Eosinophil granules are rich in N-acetyl-D-galactosamine compounds, and the colloidal gold-labeled SBA, HPA, and DBA are useful for electron microscopic detection of eosinophil granules.

scholarly journals Effects of Rocket Seed Oil, Wheat Germ Oil, and Their Mixture on Growth Performance, Feed Utilization, Digestibility, Redox Status, and Meat Fatty Acid Profile of Growing Rabbits

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 662
Author(s):  
Sabrin Abdelrahman Morshedy ◽  
Ahmed M. Abdelmodather ◽  
Mohamed M. Basyony ◽  
Soliman A. Zahran ◽  
Mohamed A. Hassan
Keyword(s):  
Immune Response ◽  
Fatty Acid ◽  
Growth Performance ◽  
Wheat Germ ◽  
Seed Oil ◽  
Average Daily Gain ◽  
Feed Utilization ◽  
Nutrient Digestibility ◽  
Control Group ◽  
Wheat Germ Oil

Vegetable oils are a source of natural antioxidants, including tocopherols, sterols, phenolic compounds, coenzymes, and polyunsaturated fatty acids that provide nutritional value, organoleptic properties, and significantly delay or prevent lipid oxidation. Eighty-four V-line rabbits at 5 weeks of age with an initial body weight (BW) of 535.60 ± 13.48 g were assigned randomly to four experimental groups (seven replicates in each group with three rabbits each). The first group served as a control and received 0.3 mL/kg BW of distilled water (CON), while the second and third groups received 0.3 mL/kg BW of rocket seed oil (RSO) and wheat germ oil (WGO), respectively. The fourth group received a mixture of oils consisting of 0.15 mL of RSO and 0.15 mL of WGO/kg BW (MOs). The experiment lasted 7 weeks. The study investigated the effects of RSO, WGO, and their mixture on growth performance, feed utilization, antioxidant status, and immune response of growing rabbits. The results indicated that the rabbits that were administered orally with RSO and WGO or their mixture had higher (p ≤ 0.05) final BW, weight gain, and average daily gain when compared to the control group. In addition, the feed conversion ratio improved significantly with RSO, WGO, and MOs treatments. Different oil treatments improved nutrient digestibility, nutritive value, and nitrogen balance. Moreover, the rabbits that received RSO, WGO, and their mixture had an improvement the meat fatty acid composition compared to the control rabbits. Oral administration of RSO, WGO, and their mixture significantly improved serum protein fractions, decreased blood urea nitrogen, and had a positive effect on serum total lipids, HDL-c, and LDL-c. Furthermore, the treatments of RSO, WGO, and MOs had a significant improvement in the antioxidative status and immune response.

Glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation.

1987 ◽  
Vol 35 (1) ◽  
pp. 33-37 ◽  
Author(s):  
H Holthöfer ◽  
I Virtanen
Keyword(s):  
Binding Sites ◽  
Mesangial Cells ◽  
Ricinus Communis ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Cell Types ◽  
Basement Membranes ◽  
Phenotypic Change ◽  
Neuraminidase Treatment ◽  
Nephron Development

Expression of cellular glycoconjugates during differentiation of human fetal kidney was studied using fluorochrome-labeled lectins. Each lectin revealed a characteristic binding pattern during the phenotypic change of the nephrogenic mesenchyme and during distinct stages of nephron development. The uninduced mesenchymal cells were positive for Pisum sativum (PSA), Concanavalin A (ConA), Wistaria floribunda (WGA), and Ricinus communis (RCA-I) lectins. However, these lectins failed to react with the uninduced cells of the S-shaped bodies, whereas Maclura pomifera (MPA), Triticum vulgaris (WGA) and, after neuraminidase treatment, Arachis hypogaea (PNA) agglutinins bound intensely to the presumptive podocytes. During later stages of nephrogenesis, MPA positively on the podocytes weakened and could not be observed in adult kidney glomeruli. Binding sites for Helix pomatia (HPA) agglutinin in glomeruli were also expressed only transiently during nephrogenesis. During further development PSA, ConA, WFA, and RCA-I reacted with mesangial cells in addition to the glomerular basement membranes. The segment-specific lectin binding patterns of the tubuli emerged in parallel with the appearance of brush border and Tamm-Horsfall antigens of the proximal and distal tubuli. The results show that nephron site-specific saccharides appear in a developmentally regulated manner and in parallel with morphologic maturation of the nephron. Lectins therefore appear to be useful tools for study of induction and maturation of various nephron cell types.

Crystallization of Helix pomatia agglutinin (HPA), a protein from the edible snail

1999 ◽  
Vol 55 (11) ◽  
pp. 1903-1905 ◽  
Author(s):  
John N. Lisgarten ◽  
James E. Pitts ◽  
Rex A. Palmer ◽  
Colin D. Reynolds ◽  
Minh Hoa Dao-Thi ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Diffraction Pattern ◽  
Helix Pomatia ◽  
Unit Cell ◽  
Hanging Drop ◽  
Helix Pomatia Agglutinin ◽  
Cell Dimensions ◽  
Protein Molecules ◽  
Unit Cell Dimensions ◽  
Drop Technique

Crystals of Helix pomatia agglutinin (HPA) have been grown by the hanging-drop technique using polyethylene glycol as the precipitant at 293 K. Over a period of one to two weeks the crystals grew to maximum dimensions of 0.10 × 0.05 × 0.02 mm. The crystals belong to space group P6322, with unit-cell dimensions a = b = 63.3, c = 105.2 Å and Z = 12 identical monomers of Mr = 13 kDa, aggregating into two 78 kDa hexameric protein molecules per unit cell, each with symmetry 32 (D 3). The diffraction pattern extends to 3.6 Å at 293 K.

Effect of Melatonin on Telocytes in the Seminal Vesicle of the Soay Ram: An Immunohistochemical, Ultrastructural and Morphometrical Study

2016 ◽  
Vol 203 (1) ◽  
pp. 29-54 ◽  
Author(s):  
Hanan H. Abd-Elhafeez ◽  
Doaa M. Mokhtar ◽  
Ahmed H.S. Hassan
Keyword(s):  
Close Contact ◽  
Intercellular Junctions ◽  
Secretory Activity ◽  
Treated Group ◽  
Control Group ◽  
Secretory Vesicles ◽  
Melatonin Treatment ◽  
Secretory Structures ◽  
Strong Reaction ◽  
Nonbreeding Season

Telocytes (TCs) are a special type of interstitial cell with characteristic cellular processes that are described in many organs. The current study aimed to investigate TCs in seminal vesicles of the Soay ram responding to melatonin treatment during the nonbreeding season by conventional immunohistochemical stains, and to detect the ultrastructural and morphometrical changes of TCs. TCs in the control group showed a broad range of staining affinity and also reacted positively to CD117/c-kit, CD34, desmin, S-100 protein, and progesterone and estrogen receptors alpha, while after melatonin treatment a strong reaction against these 6 antibodies was recorded. Electron microscopically, TCs in the control group were characterized by a small cell body with distinct long cytoplasmic extensions called telopodes (Tps). Tps had alternation of the thin segment (podomers) and dilated segments (podoms), in which the latter accommodate mitochondria, rough endoplasmic reticulum and caveolae. TCs and their Tps were interconnected by homo- and heterocellular junctions and form a wide network to communicate between different cell types. Tps showed close contact with immune cells, progenitor stem cells, smooth muscle cells and other interstitial cells. Melatonin caused a significant increase in the number of TCs, length of Tps, and number and diameter of secretory vesicles. Also, the melatonin-treated group showed exaggerated secretory activity in the form of a massive release of secretory vesicles from Tps. Moreover, Tps showed an increase in their contact with blood and lymphatic capillaries, nerve endings and Schwann cells. In addition, the shedding of secretory structures (exosomes, ectosomes, and multivesicular bodies) was greater from Tps, which were involved in paracrine signaling in the melatonin-treated group. The length and ramifications of Tps together with the intercellular junctions and the releasing of shed vesicles or exosomes assumed an essential role of TCs in intercellular signaling and coordination. On the basis of their distribution and morphology, we investigated whether the different locations of TCs could be associated with different roles.

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