scholarly journals Influence of glyceryl guaiacolate ether on anesthetics in tilapia compared to benzocaine and eugenol

2014 ◽  
pp. 3944-3953 ◽  
Author(s):  
Geovana R. Cosenza ◽  
Gustavo S. Claudiano ◽  
Paulo F. Marcusso ◽  
Silas F. Eto ◽  
Wilson G. Manrique ◽  
...  
Keyword(s):  
Total Protein ◽  
Nile Tilapia ◽  
Hematological Parameters ◽  
Running Water ◽  
Rapid Induction ◽  
Fish Blood ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Recovery Times ◽  
The Times ◽  
Hematological Changes

ABSTRACTObjective. The study aimed to investigate the effectiveness of glyceryl guaiacolate ether (GGE) and compare the times of induction, recovery, hematological changes, total protein and glycaemia among anesthetics in Nile tilapia, Oreochromis niloticus. Materials and methods. A total of 60 tilapia distributed in 3 aquariums (N=20) were used, which formed the group benzocaine (100 mg/L), eugenol (50 mg/L) and guaiacol glyceryl ether (9.000 mg/L). After the induction of anesthesia fish blood samples were collected to determine the complete hemogram and glycemia. Then the animals were placed in aquariums with running water for assessing the anesthesia recovery. Results. It was verified that GGE showed longer induction and recovery times as well a significant increase (p<0.05) of glycemia, when compared with the other groups (p<0.05). The concentration of total protein did not differ between groups (p>0.05). An increase in the number of monocytes in the group treated with benzocaine (p <0.05) was observed in the analysis of the hematological parameters with no difference between groups for other variables. Conclusions. Eugenol and benzocaine allow rapid induction and recovery in Nile tilapia, without evidence of stress during handling and GGE showed high induction and recovery times, being inadequate for anesthetic use in Nile tilapia.

scholarly journals Optimal Replacement of Fish Meal Protein by Stick Water in Diet of Sex-Reversed Nile Tilapia (Oreochromis niloticus)

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 521
Author(s):  
Uraiwan Wattanakul ◽  
Wattana Wattanakul ◽  
Karun Thongprajukaew
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Fish Meal ◽  
Specific Activity ◽  
Hematological Parameters ◽  
Dietary Treatment ◽  
Normal Liver ◽  
Carcass Quality ◽  
Liver Histopathology ◽  
Nile Tilapia Oreochromis Niloticus

The effects of replacing fish meal (FM) protein with stick water (SW) were investigated during the market stage of sex-reversed Nile tilapia, Oreochromis niloticus (18.49 ± 0.31 g initial body weight). The FM protein was replaced with SW for 10% (10SW), 20% (20SW), 30% (30SW) and 50% (50SW) of the FM. The completely randomized design was conducted in outdoor 15 floating baskets (1.5 × 1.5 × 2 m), comprising three replications with 50 fish each, over an 8 month trial. At the end of the experiment, no differences in survival, growth performance or feed utilization were observed across the dietary treatments (p > 0.05). A significant change in lipase-specific activity was caused by the replacement, without changes to trypsin, chymotrypsin or amylase activities. The fish in all dietary groups exhibited normal liver histopathology, but the fish fed a diet containing SW showed higher numbers of cells accumulating lipids as compared to fish fed the baseline 0SW dietary treatment. Hematological parameters were similar across the five dietary groups. Only fish fed the 20SW diet had superior carcass quality compared to the baseline 0SW group, in terms of crude protein and lipids, but lower or higher replacement levels had negative effects on carcass quality. Findings from the current study support the replacement of FM protein with SW at a level of 20% in the diet of sex-reversed Nile tilapia reared to the market stage. Higher replacement levels might be possible with the supplementation of fatty acids.

scholarly journals Effect of pollution with lead, cupper, cadmium on gene expression pattern of liver GST and serum lysozymes in Nile tilapia (Oreochromis. niloticus)

2020 ◽  
Vol 21 (3) ◽  
pp. 53-60
Author(s):  
Enas Hassan ◽  
Gehad El-Sayed ◽  
Azza Hassan ◽  
Shaimaa Abd Elsalam
Keyword(s):  
Gene Expression ◽  
Uric Acid ◽  
Expression Pattern ◽  
Total Protein ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Gene Expression Pattern ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Nile Tilapia Oreochromis Niloticus

Objective: This study was conducted to evaluate the influence of pollution with lead, copper, cadmium on the gene expression pattern of liver Glutathione-S-transferase and serum lysozyme in Nile tilapia (Oreochromis. niloticus). Design: Descriptive study. Fish: A Total of 120 Nile tilapia fish (Oreochromis niloticus) samples were collected from Lake Manzala, and drainage water at different localities. Procedures: Cd, Cu, and Pb concentrations residues within mid-dorsal muscle tissue, within gills, liver, and kidney were determined. Erythrocyte count, hemoglobin concentration, Packed Cell volume and other blood indices, as well as, total leukocyte count were measured. Biochemically, Alanine transaminase, Aspartate transaminase activities, total protein, creatinine, uric acid, lysozymes activity were estimated. GST gene expression was determined in the liver. Results: The results showed that Pb, Cu and Cd were bio accumulated at a higher level in the liver, kidney and gills of Nile tilapia fish (Oreochromis niloticus) from all sampling sites. The levels of the ALT and AST were increased, total protein and Albumin concentrations were decreased. Creatinine and uric acid were significantly (P≤ 0.05) increased in all groups (compared to the control group. Hematological parameters and lysozyme activity were decreased. Up regulation of the hepatic GST expression levels in Nile tilapia exposed to the heavy metals in comparison to the control value. Conclusion and clinical relevance: This study shows that lead, copper and cadmium were bio accumulated at higher concentration in liver, kidney, gills and muscles of Nile tilapia due to large industrial activities near locations of the sampling sites. Additionally, GST gene expression represents sensitive biomarker of aquatic pollution.

scholarly journals Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4237
Author(s):  
Nadia Cristine Weinert ◽  
Julieta Volpato ◽  
Ádson Costa ◽  
Rozyanne Rosa Antunes ◽  
Aldo Camargo de Oliveira ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Hematological Parameters ◽  
Prognostic Indicator ◽  
Control Group ◽  
Average Weight ◽  
Commercial Fish ◽  
Pathological Conditions ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Apparently Healthy

<p>Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (<em>Oreochromis niloticus</em>) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L- 1) (EG) and Benzocaine hydrochloride (100 mg·L-1) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na2EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ? 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na2EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na2EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species <em>Oreochromis niloticus. </em></p>

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