scholarly journals Identification of Two Types of Stem Cells in Methylene Blue-stained Sections of Untreated and Diethylstilbestrol-treated Human Prostate Cancer and Their Characterization by Immunogold Localization of CD133

2018 ◽  
Vol 38 (10) ◽  
pp. 5725-5732 ◽  
Author(s):  
AKHOURI A. SINHA ◽  
MICHAEL J. WILSON
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Methylene Blue ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Immunogold Localization

Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 2138-2149 ◽  
Author(s):  
Günnur Güler ◽  
Ummu Guven ◽  
Gulperi Oktem
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Ftir Spectroscopy ◽  
Molecular Mechanisms ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Stem Cells

Molecular mechanisms and features of prostate cancer stem cells, which are crucial for improving target specific therapies, were elucidated with ATR-FTIR spectroscopy.

scholarly journals Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

10.3791/51332 ◽  
2014 ◽  
Author(s):  
Samuel J. Vidal ◽  
S. Aidan Quinn ◽  
Janis de la Iglesia-Vicente ◽  
Dennis M. Bonal ◽  
Veronica Rodriguez-Bravo ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Human Prostate ◽  
Human Prostate Cancer

275: Bi-Directional Interactions of Bone Marrow Mesenchymal Stem Cells with Human Prostate Cancer Cells

2007 ◽  
Vol 177 (4S) ◽  
pp. 92-92 ◽  
Author(s):  
Chunmeng Shi ◽  
Ying Zhu ◽  
Wen-Chin Huang ◽  
Haiyen E. Zhau ◽  
Ruoxiang Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Marrow Mesenchymal Stem Cells

scholarly journals KCa1.1 K+ Channel Inhibition Overcomes Resistance to Antiandrogens and Doxorubicin in a Human Prostate Cancer LNCaP Spheroid Model

2021 ◽  
Vol 22 (24) ◽  
pp. 13553
Author(s):  
Susumu Ohya ◽  
Junko Kajikuri ◽  
Kyoko Endo ◽  
Hiroaki Kito ◽  
Miki Matsui
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Protein Degradation ◽  
Human Prostate ◽  
K Channel ◽  
Stem Cell Markers ◽  
Human Prostate Cancer ◽  
Spheroid Formation ◽  
Isolated Cells ◽  
Channel Inhibition

Several types of K+ channels play crucial roles in tumorigenicity, stemness, invasiveness, and drug resistance in cancer. Spheroid formation of human prostate cancer (PC) LNCaP cells with ultra-low attachment surface cultureware induced the up-regulation of cancer stem cell markers, such as NANOG, and decreased the protein degradation of the Ca2+-activated K+ channel KCa1.1 by down-regulating the E3 ubiquitin ligase, FBXW7, compared with LNCaP monolayers. Accordingly, KCa1.1 activator-induced hyperpolarizing responses were larger in isolated cells from LNCaP spheroids. The pharmacological inhibition of KCa1.1 overcame the resistance of LNCaP spheroids to antiandrogens and doxorubicin (DOX). The protein expression of androgen receptors (AR) was significantly decreased by LNCaP spheroid formation and reversed by KCa1.1 inhibition. The pharmacological and genetic inhibition of MDM2, which may be related to AR protein degradation in PC stem cells, revealed that MDM2 was responsible for the acquisition of antiandrogen resistance in LNCaP spheroids, which was overcome by KCa1.1 inhibition. Furthermore, a member of the multidrug resistance-associated protein subfamily of ABC transporters, MRP5 was responsible for the acquisition of DOX resistance in LNCaP spheroids, which was also overcome by KCa1.1 inhibition. Collectively, the present results suggest the potential of KCa1.1 in LNCaP spheroids, which mimic PC stem cells, as a therapeutic target for overcoming antiandrogen- and DOX-resistance in PC cells.

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