scholarly journals Development of a Novel Experimental In Vitro Model of Isothiocyanate-induced Apoptosis in Human Malignant Melanoma Cells

2016 ◽  
Vol 36 (12) ◽  
pp. 6303-6310 ◽  
Author(s):  
THEODORA MANTSO ◽  
ARISTEIDIS P SFAKIANOS ◽  
AITHNE ATKINSON ◽  
IOANNIS ANESTOPOULOS ◽  
MELINA MITSIOGIANNI ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
In Vitro Model ◽  
Melanoma Cells ◽  
Human Malignant Melanoma ◽  
Vitro Model ◽  
Induced Apoptosis

scholarly journals Anticancer activity of a novel methylated analogue of L-mimosine against an in vitro model of human malignant melanoma

2019 ◽  
Vol 38 (3) ◽  
pp. 621-633
Author(s):  
Sotiris Kyriakou ◽  
Melina Mitsiogianni ◽  
Theodora Mantso ◽  
William Cheung ◽  
Stephen Todryk ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Anticancer Activity ◽  
In Vitro Model ◽  
Human Malignant Melanoma ◽  
Vitro Model

scholarly journals A novel methylated analogue of L-Mimosine exerts its therapeutic potency through ROS production and ceramide-induced apoptosis in malignant melanoma

2021 ◽  
Author(s):  
Sotiris Kyriakou ◽  
William Cheung ◽  
Theodora Mantso ◽  
Melina Mitsiogianni ◽  
Ioannis Anestopoulos ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
In Vitro Model ◽  
Ros Generation ◽  
Intrinsic Apoptosis ◽  
Membrane Depolarisation ◽  
Vitro Model ◽  
Induced Apoptosis ◽  
Related Proteins ◽  
Metabolomic Approach

SummaryMelanoma is an aggressive and highly metastatic type of skin cancer where the design of new therapies is of utmost importance for the clinical management of the disease. Thus, we have aimed to investigate the mode of action by which a novel methylated analogue of L-Mimosine (e.g., L-SK-4) exerts its therapeutic potency in an in vitro model of malignant melanoma. Cytotoxicity was assessed by the Alamar Blue assay, oxidative stress by commercially available kits, ROS generation, caspase 3/7 activation and mitochondrial membrane depolarisation by flow cytometry, expression of apoptosis-related proteins by western immunoblotting and profiling of lipid biosynthesis by a metabolomic approach. Overall, higher levels of ROS, sphingolipids and apoptosis were induced by L-SK-4 suggesting that the compound’s therapeutic potency is mediated through elevated ROS levels which promote the upregulation of sphingolipid (ceramide) biosynthesis thus leading to the activation of both extrinsic and intrinsic apoptosis, in an experimental model of malignant melanoma.

scholarly journals Radiation respones of HMV-I human malignant melanoma cells grown in vitro as multicellular spheroids.

1984 ◽  
Vol 25 (3) ◽  
pp. 215-224 ◽  
Author(s):  
NOBUO KUBOTA ◽  
KENGO MATSUI ◽  
SHINICHIRO SATO ◽  
TETSUO INADA
Keyword(s):  
Malignant Melanoma ◽  
Melanoma Cells ◽  
Multicellular Spheroids ◽  
Human Malignant Melanoma

The Activity of Urolithin A and M4 Valerolactone, Colonic Microbiota Metabolites of Polyphenols, in a Prostate Cancer In Vitro Model

Planta Medica ◽  
2018 ◽  
Vol 85 (02) ◽  
pp. 118-125 ◽  
Author(s):  
Iwona Stanisławska ◽  
Sebastian Granica ◽  
Jakub Piwowarski ◽  
Joanna Szawkało ◽  
Krzysztof Wiązecki ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
In Vitro Model ◽  
Specific Antigen ◽  
Lncap Cells ◽  
Isobolographic Analysis ◽  
Human System ◽  
Urolithin A ◽  
Vitro Model ◽  
Induced Apoptosis

AbstractThe gut microbiota-derived metabolites of ellagitannins and green tea catechins, urolithin A (uroA) and 5-(3′,4′,5′-trihydroxyphenyl)-γ-valerolactone (M4), respectively, are among the main compounds absorbed into human system after ingestion of these polyphenols. The aim of this study was to establish the effects of M4, uroA, and their combinations on LNCaP cells, an androgen dependent prostate cancer in vitro model.. The LNCaP cells were incubated with increasing concentrations of tested metabolites. The cell proliferation was determined by measurement of DNA-bisbenzimide H 33 258 complexes fluorescence. The isobolographic analysis was used to establish the type of interaction between metabolites. The apoptosis, androgen receptor (AR) localization, and phosphorylation of Akt kinase were measured by flow cytometry. Prostate-specific antigen (PSA) secretion was determined by ELISA. M4 showed modest antiproliferative activity in LNCaP cells (IC50 = 117 µM; CI: 81 – 154). UroA decreased proliferation (IC50 = 32.7 µM; CI: 24.3 – 41.1) and induced apoptosis of LNCaP cells. The mixture of M4 with uroA had synergistic antiproliferative effect. Moreover, M4 potentiated inhibition of PSA secretion and enhanced retention of AR in cytoplasm caused by uroA. Interestingly, uroA increased levels of pSer473 Akt in LNCaP cells. These results show that colonic metabolites may contribute to chemoprevention of prostate cancer by varied polyphenol-rich diet or composite polyphenol preparations.

A New and Lifelike In Vitro Model of Malignant Melanoma

1989 ◽  
Vol 11 (3) ◽  
pp. 285
Author(s):  
A. Majid ◽  
P. W.M. Copeman ◽  
K. Henry
Keyword(s):  
Malignant Melanoma ◽  
In Vitro Model ◽  
Vitro Model
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