scholarly journals Bone Mineral Density and Cytoplasmic Free Calcium Concentration in Urinary Granulocytes of the Aged Population in a Rural Area of Southern Nagano Prefecture.

2000 ◽  
Vol 49 (2) ◽  
pp. 98-104
Author(s):  
Nobuo NAGAI ◽  
Akio HIROSE ◽  
Sachihiro YAMADA
Keyword(s):  
Bone Mineral Density ◽  
Rural Area ◽  
Bone Mineral ◽  
Calcium Concentration ◽  
Free Calcium ◽  
Mineral Density ◽  
Nagano Prefecture ◽  
Aged Population ◽  
Free Calcium Concentration ◽  
Cytoplasmic Free Calcium

Cytoplasmic free calcium concentration in porcine platelets

1984 ◽  
Vol 800 (2) ◽  
pp. 178-187 ◽  
Author(s):  
A. David Purdon ◽  
James L. Daniel ◽  
Gwendolyn J. Stewart ◽  
Holm Holmsen
Keyword(s):  
Calcium Concentration ◽  
Free Calcium ◽  
Free Calcium Concentration ◽  
Cytoplasmic Free Calcium

The Effects of Chloride Channel Blockers on Platelet Cytoplasmic Free Calcium Concentration and Platelet Aggregation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3879-3879
Author(s):  
Songmei Yin ◽  
Xiaolin Chen ◽  
Danian Nie ◽  
Shuangfeng Xie ◽  
Liping Ma ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Calcium Channel ◽  
Calcium Channel Blockers ◽  
Chloride Channel ◽  
Calcium Concentration ◽  
Disulfonic Acid ◽  
Free Calcium ◽  
Channel Blockers ◽  
Free Calcium Concentration ◽  
Cytoplasmic Free Calcium

Abstract Objective To explore the effects of chloride channels on the regulations of platelet cytoplasmic free calcium concentration ([Ca2+]i) and platelet aggregation (PAG). Methods Platelet were separated freshly and then activated by thrombin; The chloride channel blockers 4,4′-diisothiocyano-2, 2′-disulfonic acid stilbene (DIDS) or niflumic acid (NFA), and calcium channel blockers 1-{β-[3-(4-methoxyphenyl)propoxy]- 4-methoxyphenethyl}- 1H - imidazole hydrochloride (SK&F96365) or Nifedipine were added to react with the activated platelets. The effects of each agent on platelet [Ca2+]i and PAG were detected. The combine effects and the interactions among chloride channel blockers (DIDS, NFA) and calcium channel blockers (SK&F96365, Nifedipine) were also investigated. Results Both DIDS and NFA [the concentration were12.5, 25, 50, 100 and 200μmol•L−1 respectively] could inhibit the PAG induced by thrombin (1U/ml) and the effect was dose-dependent. Compared with the control, they had no significant effects on resting [Ca2+]i. Compare with the control group, DIDS (100μmol•L−1), SK&F96365 (100μmol•L−1) and Nifedipine (100μmol•L−1) could significantly reduce the PAG, Ca2+ release and Ca2+ influx activated by thrombin in platelet (P<0.05). DIDS (100μmol•L−1) and SK&F96365 (100μmol•L−1) could enhance each other’s effect on reducing the PAG, Ca2+ release and Ca2+ influx (P<0.05). DIDS (100μmol•L−1) and Nifedipine (100μmol•L−1) could enhance each other’s effect on reducing Ca2+ release (P<0.05). NFA (100μmol•L−1) and SK&F96365 (100μmol•L−1) could weaken each other’s effect on Ca2+ release (P<0.05). NFA (100μmol•L−1) and Nifedipine (100μmol•L−1) could weaken each other’s effect on PAG, Ca2+ release and Ca2+ influx activated by thrombin in platelet (P<0.05). Conclusion The chloride channel blockers DIDS and NFA have no effect on the resting [Ca2+]i and the leak calcium influx of platelet. DIDS can inhibit the Ca2+ release, Ca2+ influx and PAG of platelet induced by thrombin, while NFA can only inhibit the Ca2+ release of platelet induced by thrombin. There are interactions between chloride channel blockers and calcium channel blockers in resting [Ca2+]i and PAG of platelet. The opening of chloride channel can influence the cellular calcium movement of platelet.

scholarly journals Bone mineral density in adolescents: Higher values in a rural area—a population-based study of 246 subjects in southern Sweden

1997 ◽  
Vol 68 (5) ◽  
pp. 456-460 ◽  
Author(s):  
Martin Sundberg ◽  
Henrik Düppe ◽  
Per Gárdsell ◽  
Olof Johnell ◽  
Ewald Ornstein ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Rural Area ◽  
Bone Mineral ◽  
Population Based ◽  
Southern Sweden ◽  
Mineral Density ◽  
Population Based Study
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