scholarly journals Functional Control of a Living Cell by Femtosecond Laser.

2001 ◽  
Vol 29 (11) ◽  
pp. 730-733
Author(s):  
Osamu NAKAMURA ◽  
Tomoyuki KANEKO
Keyword(s):  
Femtosecond Laser ◽  
Living Cell ◽  
Functional Control

Noncontact microsurgery of living cell membrane using femtosecond laser pulses

2013 ◽  
Author(s):  
I. V. Ilina ◽  
A. V. Ovchinnikov ◽  
D. S. Sitnikov ◽  
O. V. Chefonov ◽  
M. B. Agranat ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Femtosecond Laser ◽  
Living Cell ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses

scholarly journals Femtosecond laser disruption of subcellular organelles in a living cell

2004 ◽  
Vol 12 (18) ◽  
pp. 4203 ◽  
Author(s):  
Wataru Watanabe ◽  
Naomi Arakawa ◽  
Sachihiro Matsunaga ◽  
Tsunehito Higashi ◽  
Kiichi Fukui ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Living Cell ◽  
Subcellular Organelles

In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser

2008 ◽  
Vol 254 (11) ◽  
pp. 3370-3375 ◽  
Author(s):  
Sung-Hak Cho ◽  
Won-Seok Chang ◽  
Jae-Goo Kim ◽  
Kyoung-Hyun Whang ◽  
Kyeong-Sook Choi ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Living Cell ◽  
In Situ Observation ◽  
Single Living Cell ◽  
Single Living

Measurement of UV absorption of single living cell for cell manipulation using NIR femtosecond laser

2009 ◽  
Vol 255 (9) ◽  
pp. 4974-4978 ◽  
Author(s):  
Sung-Hak Cho ◽  
Won-Seok Chang ◽  
Kwang-Ryul Kim ◽  
Jong Wook Hong
Keyword(s):  
Femtosecond Laser ◽  
Living Cell ◽  
Uv Absorption ◽  
Cell Manipulation ◽  
Single Living Cell ◽  
Single Living

scholarly journals Manipulation of organelles in a living cell using a femtosecond laser

2006 ◽  
Vol 34 (Supplement) ◽  
pp. 259-260
Author(s):  
W. Watanabe ◽  
T. Shimada ◽  
S. Matsunaga ◽  
T. Higashi ◽  
H. Ishii ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Living Cell

scholarly journals Femtosecond laser processing in water for single living cell and solid phase protein

2007 ◽  
Vol 35 (Supplement) ◽  
pp. 246-247
Author(s):  
Yoichiroh Hosokawa ◽  
Ryohei Yasukuni ◽  
Takahiro Kaji ◽  
Yuji Hiraki ◽  
Hajime Mori ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Processing ◽  
Living Cell ◽  
Solid Phase ◽  
Single Living Cell ◽  
Femtosecond Laser Processing ◽  
Phase Protein ◽  
Single Living

Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 14-15
Author(s):  
Conly L. Rieder
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Light Microscopy ◽  
Living Cell ◽  
Light And Electron Microscopy ◽  
Time Behavior ◽  
Dynamic Interactions ◽  
Positive Identification ◽  
Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

Application of high-resolution field-emission LVSEM, backscatter imaging, immunogold staining to definition of the spatial distributions of two human neutrophil adherence receptors

1993 ◽  
Vol 51 ◽  
pp. 36-37
Author(s):  
Robert D. Nelson ◽  
Sharon R. Hasslen ◽  
Stanley L. Erlandsen
Keyword(s):  
Cell Surface ◽  
Surface Membrane ◽  
Three Dimensional ◽  
Human Neutrophils ◽  
Spatial Distributions ◽  
Immunogold Staining ◽  
Functional Control ◽  
Neutrophil Adherence ◽  
Definition Of ◽  
Mode Of Attachment

Receptors are commonly defined in terms of number per cell, affinity for ligand, chemical structure, mode of attachment to the cell surface, and mechanism of signal transduction. We propose to show that knowledge of spatial distribution of receptors on the cell surface can provide additional clues to their function and components of functional control.L-selectin and Mac-1 denote two receptor populations on the neutrophil surface that mediate neutrophil-endothelial cell adherence interactions and provide for targeting of neutrophil recruitment to sites of inflammation. We have studied the spatial distributions of these receptors using LVSEM and backscatter imaging of isolated human neutrophils stained with mouse anti-receptor (primary) antibody and goat anti-mouse (secondary) antibody conjugated to 12 nm colloidal gold. This combination of techniques provides for three-dimensional analysis of the expression of these receptors on different surface membrane domains of the neutrophil: the ruffles and microvilli that project from the cell surface, and the cell body between these projecting structures.

Nanosecond laser-induced breakdown assisted by femtosecond laser pre-ionization in air: the effect on spatial resolution and continuous radiation

2020 ◽  
Vol 92 (2) ◽  
pp. 20701
Author(s):  
Bo Li ◽  
Xiaofeng Li ◽  
Zhifeng Zhu ◽  
Qiang Gao
Keyword(s):  
Femtosecond Laser ◽  
Spatial Resolution ◽  
Laser Induced Breakdown Spectroscopy ◽  
Nanosecond Laser ◽  
Breakdown Threshold ◽  
Powerful Technique ◽  
Breakdown Spectroscopy ◽  
Continuous Radiation ◽  
Laser Induced Breakdown

Laser-induced breakdown spectroscopy (LIBS) is a powerful technique for quantitative diagnostics of gases. The spatial resolution of LIBS, however, is limited by the volume of plasma. Here femtosecond-nanosecond dual-pulsed LIBS was demonstrated. Using this method, the breakdown threshold was reduced by 80%, and decay of continuous radiation was shortened. In addition, the volume of the plasma was shrunk by 85% and hence, the spatial resolution of LIBS was significantly improved.

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