scholarly journals Pilot study on passive sensitization against ovalbumin using equine Precision Cut Lung Slices (PCLS)

2010 ◽  
Vol 26 (6) ◽  
pp. 789-796
Author(s):  
A K Barton ◽  
F Niedorf ◽  
J Rohwer ◽  
M Kietzmann ◽  
B Ohnesorge
Keyword(s):  
Pilot Study ◽  
Passive Sensitization ◽  
Precision Cut Lung Slices

Comparison of Long-term Human Precision-cut Lung Slice Culture Methodology and Response to Challenge: An Argument for Standardisation

2021 ◽  
pp. 026119292110618
Author(s):  
Vivek Patel ◽  
Khalid Amin ◽  
David Allen ◽  
Lindsey Ukishima ◽  
Adam Wahab ◽  
...  
Keyword(s):  
Pilot Study ◽  
Culture Conditions ◽  
Cytokine Response ◽  
Poly I:C ◽  
Slice Culture ◽  
Functional Responses ◽  
Culture Methods ◽  
Animal Alternatives ◽  
Precision Cut Lung Slices ◽  
Air Liquid Interface

As non-animal alternatives gain acceptance, a need for harmonised testing strategies has emerged. Arguably the most physiologically-relevant model for assessing potential respiratory toxicants, that based on human precision-cut lung slices (hPCLS) has been utilised in many laboratories, but a variety of culture methodologies are employed. In this pilot study, combinations of three different hPCLS culture methods (dynamic organ roller culture (DOC), air–liquid interface (ALI) and submersion) and various media (based on E-199, DMEM/F12 and RPMI-1640) were compared. The hPCLS were assessed in terms of their viability and responsiveness to challenge. The endpoints selected to compare the medium–method (M–M) combinations, which included histological features and viability, were evaluated at day 14 (D14) and day 28 (D28); protein and adenylate kinase (AK) content, and cytokine response to immunostimulants (lipopolysaccharide (LPS) at 5 μg/ml; polyinosinic:polycytidylic acid (Poly I:C) at 15 μg/ml) were evaluated at D28 only. Based on the set of endpoints assessed at D28, it was clear that certain culture conditions significantly affected the hPCLS, with the tissue retaining more of its native features and functionality (in terms of cytokine response) in some of the M–M combinations tested more than others. This pilot study indicates that the use of appropriate M–M combinations can help maintain the health and functional responses of hPCLS, and highlights the need for the standardisation of culture conditions in order to facilitate effective inter-laboratory comparisons and encourage greater acceptance by the regulatory community.

Pilot study on criteria in cavity preparation-facts or artifacts?

1973 ◽  
Vol 37 (11) ◽  
pp. 27-31 ◽  
Author(s):  
G Salvendy ◽  
WM Hinton ◽  
GW Ferguson ◽  
PR Cunningham
Keyword(s):  
Pilot Study ◽  
Cavity Preparation

A Pilot Study of Early Storybook Reading With Babies With Hearing Loss

2019 ◽  
Vol 62 (9) ◽  
pp. 3397-3412
Author(s):  
Michelle I. Brown ◽  
David Trembath ◽  
Marleen F. Westerveld ◽  
Gail T. Gillon
Keyword(s):  
Hearing Loss ◽  
Pilot Study ◽  
Visual Analysis ◽  
Single Case ◽  
Eye Contact ◽  
Preliminary Evidence ◽  
Storybook Reading ◽  
Book Selection ◽  
Turn Taking ◽  
Parent Child

Purpose This pilot study explored the effectiveness of an early storybook reading (ESR) intervention for parents with babies with hearing loss (HL) for improving (a) parents' book selection skills, (b) parent–child eye contact, and (c) parent–child turn-taking. Advancing research into ESR, this study examined whether the benefits from an ESR intervention reported for babies without HL were also observed in babies with HL. Method Four mother–baby dyads participated in a multiple baseline single-case experimental design across behaviors. Treatment effects for parents' book selection skills, parent–child eye contact, and parent–child turn-taking were examined using visual analysis and Tau-U analysis. Results Statistically significant increases, with large to very large effect sizes, were observed for all 4 participants for parent–child eye contact and parent–child turn-taking. Limited improvements with ceiling effects were observed for parents' book selection skills. Conclusion The findings provide preliminary evidence for the effectiveness of an ESR intervention for babies with HL for promoting parent–child interactions through eye contact and turn-taking.

Effect of billary sphincterotomy on serum lipid profile: A pilot study

2001 ◽  
Vol 120 (5) ◽  
pp. A576-A576
Author(s):  
E FOGEL ◽  
T IMPERIALE ◽  
B DEVERAUX ◽  
S SHERMAN ◽  
J WATKINS ◽  
...  
Keyword(s):  
Pilot Study ◽  
Lipid Profile ◽  
Serum Lipid ◽  
Serum Lipid Profile

Sa1640 NONINVASIVE MEASUREMENT OF SMALL BOWEL SLOW WAVE ACTIVITY IN NEONATES - A PILOT STUDY

2020 ◽  
Vol 158 (6) ◽  
pp. S-364
Author(s):  
Suseela Somarajan ◽  
Nicole D. Muszynski ◽  
Aurelia s. Monk ◽  
Joseph D. Olson ◽  
Alexandra Russell ◽  
...  
Keyword(s):  
Pilot Study ◽  
Small Bowel ◽  
Slow Wave ◽  
Wave Activity ◽  
Slow Wave Activity ◽  
Noninvasive Measurement
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