scholarly journals Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome

2009 ◽  
Vol 85 (8) ◽  
pp. 348-362 ◽  
Author(s):  
Noemi FUSAKI ◽  
Hiroshi BAN ◽  
Akiyo NISHIYAMA ◽  
Koichi SAEKI ◽  
Mamoru HASEGAWA
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Sendai Virus ◽  
Rna Virus ◽  
Human Pluripotent Stem Cells ◽  
Host Genome ◽  
Efficient Induction

scholarly journals A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dane Z. Hazelbaker ◽  
Amanda Beccard ◽  
Gabriella Angelini ◽  
Patrizia Mazzucato ◽  
Angelica Messana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Piggybac Transposon ◽  
Efficient Induction

scholarly journals Rapid and Efficient Induction of Functional Astrocytes from Human Pluripotent Stem Cells

2018 ◽  
Author(s):  
Isaac Canals ◽  
Isaac Canals ◽  
Aurélie Ginisty ◽  
Ella Quist ◽  
Raissa Timmerman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Efficient Induction

scholarly journals Author Correction: Rapid and efficient induction of functional astrocytes from human pluripotent stem cells

2018 ◽  
Vol 16 (1) ◽  
pp. 134-134 ◽  
Author(s):  
Isaac Canals ◽  
Aurélie Ginisty ◽  
Ella Quist ◽  
Raissa Timmerman ◽  
Jonas Fritze ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Efficient Induction

Rapid and efficient induction of functional astrocytes from human pluripotent stem cells

2018 ◽  
Vol 15 (9) ◽  
pp. 693-696 ◽  
Author(s):  
Isaac Canals ◽  
Aurélie Ginisty ◽  
Ella Quist ◽  
Raissa Timmerman ◽  
Jonas Fritze ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Efficient Induction

Reprogramming fibroblasts and mononuclear blood cells into iPSCs using Sendai virus v1

2021 ◽  
Author(s):  
Dmitry Ovchinnikov
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Sendai Virus ◽  
Rna Virus ◽  
Suspension Cells ◽  
Viral Particles ◽  
Wide Range ◽  
Induced Pluripotent ◽  
Mononuclear Blood Cells

Reprogramming primary blood mononuclear suspension cells into induced pluripotent stem cells using a non-integrating RNA viral (Sendai) reprogramming factor delivery method. This ™ CytoTune-iPS 2.0 is a self-replicating RNA virus-based reprogramming approach which utilizes 3 pre-packaged viral particles expressing human KLF-4+OCT4/POU5F1+SOX2, hKLF-4 and hc-Myc proteins respectively. Use of Sendai viral particles for delivery allows for an efficient non-viral reprogramming of suspension blood and a wide range of other cells into induced pluripotent stem cells (iPSCs). This protocol describes the preparation of primary cell (blood-derived) cultures and their subsequent reprogramming into iPSCs using Sendai virus transduction.

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