scholarly journals In vitro synthesis of infectious RNA molecules from cloned hop stunt viroid complementary DNA.

1983 ◽  
Vol 59 (8) ◽  
pp. 251-254 ◽  
Author(s):  
Takeshi OHNO ◽  
Masayuki ISHIKAWA ◽  
Nobuhiko TAKAMATSU ◽  
Tetsuo MESHI ◽  
Yoshimi OKADA ◽  
...  
Keyword(s):  
In Vitro Synthesis ◽  
Complementary Dna ◽  
Rna Molecules ◽  
Stunt Viroid ◽  
Hop Stunt Viroid

scholarly journals Identification of an In Vitro Ribonucleoprotein Complex Between a Viroid RNA and a Phloem Protein from Cucumber Plants

2001 ◽  
Vol 14 (7) ◽  
pp. 910-913 ◽  
Author(s):  
Gustavo Gómez ◽  
Vicente Pallás
Keyword(s):  
Rna Binding ◽  
High Salt ◽  
Phloem Exudate ◽  
Binding Properties ◽  
Ribonucleoprotein Complex ◽  
Rna Molecules ◽  
Stunt Viroid ◽  
Phloem Protein ◽  
Hop Stunt Viroid

We used the interaction of Hop stunt viroid (HSVd) and cucumber plants to investigate the involvement of phloem proteins in the systemic transport of RNA molecules. A ribonucleoprotein complex, stable even at high salt and temperature conditions, was detected in vitro between HSVd-RNA and the phloem exudate obtained from sectioned internodes from cucumber plants. The phloem protein 2 was recovered from this ribonucleoprotein complex and its RNA-binding properties as demonstrated by gel retardation analysis. The involvement of this protein in the movement of RNAs in cucumber is discussed.

scholarly journals Mechanism of in vitro synthesis of covalently linked dimeric RNA molecules by the poliovirus replicase.

1986 ◽  
Vol 58 (2) ◽  
pp. 459-467 ◽  
Author(s):  
J M Lubinski ◽  
G Kaplan ◽  
V R Racaniello ◽  
A Dasgupta
Keyword(s):  
In Vitro Synthesis ◽  
Rna Molecules ◽  
Covalently Linked

scholarly journals A Long-Distance Translocatable Phloem Protein from Cucumber Forms a Ribonucleoprotein Complex In Vivo with Hop Stunt Viroid RNA

2004 ◽  
Vol 78 (18) ◽  
pp. 10104-10110 ◽  
Author(s):  
Gustavo Gómez ◽  
Vicente Pallás
Keyword(s):  
Rna Binding ◽  
Direct Interaction ◽  
Binding Motif ◽  
Long Distance ◽  
Rna Molecules ◽  
Stunt Viroid ◽  
Phloem Protein ◽  
Hop Stunt Viroid ◽  
Long Distance Movement

ABSTRACT Viroids are highly structured plant pathogenic RNAs that do not code for any protein, and thus, their long-distance movement within the plant must be mediated by direct interaction with cellular factors, the nature of which is presently unknown. In addition to this type of RNAs, recent evidence indicates that endogenous RNAs move through the phloem acting as macromolecular signals involved in plant defense and development. The form in which these RNA molecules are transported to distal parts of the plant is unclear. Viroids can be a good model system to try to identify translocatable proteins that could assist the vascular movement of RNA molecules. Here, we demonstrate by use of immunoprecipitation experiments, that the phloem protein 2 from cucumber (CsPP2) is able to interact in vivo with a viroid RNA. Intergeneric graft assays revealed that both the CsPP2 and the Hop stunt viroid RNA were translocated to the scion. The translocated viroid is symptomatic in the nonhost scion, indicating that the translocated RNA is functional. The CsPP2 gene was cloned and sequenced. The analysis of its primary structure revealed the existence of a potential double-spaced-RNA-binding motif, previously identified in a set of proteins that bind to highly structured RNAs, which could explain its RNA-binding properties. The possible involvement of this phloem protein in assisting the long-distance movement of the viroid RNA within the plant is discussed.

scholarly journals Simplified in vitro synthesis of mutated RNA molecules

FEBS Letters ◽  
1987 ◽  
Vol 212 (2) ◽  
pp. 271-275 ◽  
Author(s):  
Guido Krupp ◽  
Dieter Söll
Keyword(s):  
In Vitro Synthesis ◽  
Rna Molecules
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