Next-Generation Sequencing Sheds New Light on Small RNAs in Plant Reproductive Development

2018 ◽  
pp. 143-170 ◽  
Author(s):  
Xiaobai Li
Keyword(s):  
Next Generation Sequencing ◽  
Small Rnas ◽  
Reproductive Development ◽  
Next Generation ◽  
Generation Sequencing ◽  
Plant Reproductive Development

scholarly journals Next-generation sequencing reveals two populations of damage-induced small RNAs at endogenous DNA double-strand breaks

2018 ◽  
Vol 46 (22) ◽  
pp. 11869-11882 ◽  
Author(s):  
Franziska Bonath ◽  
Judit Domingo-Prim ◽  
Marcel Tarbier ◽  
Marc R Friedländer ◽  
Neus Visa
Keyword(s):  
Next Generation Sequencing ◽  
Small Rnas ◽  
Double Strand Breaks ◽  
Next Generation ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Two Populations ◽  
Generation Sequencing

scholarly journals Next-generation sequencing of small RNAs from inner ear sensory epithelium identifies microRNAs and defines regulatory pathways

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 484 ◽  
Author(s):  
Anya Rudnicki ◽  
Ofer Isakov ◽  
Kathy Ushakov ◽  
Shaked Shivatzki ◽  
Inbal Weiss ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Inner Ear ◽  
Small Rnas ◽  
Sensory Epithelium ◽  
Next Generation ◽  
Regulatory Pathways ◽  
Generation Sequencing

scholarly journals Extracting Small RNAs from Human Biological Fluids for Subsequent Next-Generation Sequencing

2019 ◽  
Vol 9 (1) ◽  
pp. 80-86
Author(s):  
O. A. Beylerli ◽  
A. T Beylerli ◽  
I. F. Garaev
Keyword(s):  
Next Generation Sequencing ◽  
Small Rnas ◽  
Biological Fluids ◽  
Rna Extraction ◽  
Small Sample ◽  
Next Generation ◽  
Illumina Platform ◽  
Small Noncoding Rnas ◽  
Organic Extraction ◽  
Generation Sequencing

A number of questions arise when choosing methods for experiments related to next-generation sequencing. On the one hand, while working with RNA extraction, added reagents and their residues can often inhibit sensitive chemicals with which the sequential synthesis is carried out for the sequencing. On the other hand, processing the same data using different software for the analysis can also impact on the sequencing results. This paper will present the step by step procedure for the preparation of samples taken from human biological fluids for subsequent sequencing of small RNAs, small noncoding RNAs in particular. Regarding the methods of extraction or isolation of RNAs, we found that low RNA yield can be improved significantly by following the isolation method for total RNA and its fractions included in Ambion’s MirVana PARIS kit, but only if using a special approach and modifying the organic extraction step. Compared to others, the methods supplied with commercially available kits at the time of researching this paper require only one organic extraction. This simple but, as it turned out, very useful modification makes it possible to access previously unavailable material. Potential advantages of this modification include a more complete profiling of small non-coding RNAs and a broader access to small sample volumes, as a rule, access to human biological fluids which can be prepared for RNA sequencing on the Illumina platform.

P4044 Search for polymorphisms through next-generation sequencing of genes involved in reproductive development in Guzerat bulls

2016 ◽  
Vol 94 (suppl_4) ◽  
pp. 100-101
Author(s):  
J. P. Liron ◽  
A. M. Loaiza Echeveri ◽  
M. E. Fernandez ◽  
M. Drummond ◽  
D. Goszczynski ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Reproductive Development ◽  
Next Generation ◽  
Generation Sequencing
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