scholarly journals MALDI-TOF MS in the Microbiology Laboratory: Current Trends

2017 ◽  
pp. 17-20 ◽  
Author(s):  
Sören Schubert ◽  
Markus Kostrzewa
Keyword(s):  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Current Trends ◽  
Tof Ms

Identification and diversity of Actinomyces species in a clinical microbiology laboratory in the MALDI-TOF MS era

Anaerobe ◽  
2018 ◽  
Vol 54 ◽  
pp. 151-158 ◽  
Author(s):  
Peivern Fong ◽  
Michelle J. Francis ◽  
John F. Hamblin ◽  
Tony M. Korman ◽  
Maryza Graham
Keyword(s):  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Actinomyces Species ◽  
Tof Ms

scholarly journals Evaluation of MALDI-TOF MS in the microbiology laboratory

2013 ◽  
Vol 49 (3) ◽  
pp. 191-197 ◽  
Author(s):  
Anderson Fernandes Santos ◽  
Rodrigo Cayô ◽  
Lygia Schandert ◽  
Ana Cristina Gales
Keyword(s):  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Comparative evaluation of matrix-assisted laser desorption ionisation-time of flight mass spectrometry and conventional phenotypic-based methods for identification of clinically important yeasts in a UK-based medical microbiology laboratory

2015 ◽  
Vol 68 (12) ◽  
pp. 1040-1042 ◽  
Author(s):  
Nita Fatania ◽  
Mark Fraser ◽  
Mike Savage ◽  
Jason Hart ◽  
Alireza Abdolrasouli
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Phenotypic Methods ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

AimsPerformance of matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) was compared in a side-by side-analysis with conventional phenotypic methods currently in use in our laboratory for identification of yeasts in a routine diagnostic setting.MethodsA diverse collection of 200 clinically important yeasts (19 species, five genera) were identified by both methods using standard protocols. Discordant or unreliable identifications were resolved by sequencing of the internal transcribed spacer region of the rRNA gene.ResultsMALDI-TOF and conventional methods were in agreement for 182 isolates (91%) with correct identification to species level. Eighteen discordant results (9%) were due to rarely encountered species, hence the difficulty in their identification using traditional phenotypic methods.ConclusionsMALDI-TOF MS enabled rapid, reliable and accurate identification of clinically important yeasts in a routine diagnostic microbiology laboratory. Isolates with rare, unusual or low probability identifications should be confirmed using robust molecular methods.

scholarly journals MALDI-TOF MS in the clinical microbiology laboratory

2013 ◽  
Vol 49 (4) ◽  
pp. 256-259 ◽  
Author(s):  
Marcelo Jenne Mimica ◽  
Marines Dalla Valle Martino ◽  
Jacyr Pasternak
Keyword(s):  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Use of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry analyser in a diagnostic microbiology laboratory in a developing country

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Atang Bulane ◽  
Anwar Hoosen
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Blood Cultures ◽  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Background: Rapid and accurate identification of pathogens is of utmost importance for management of patients. Current identification relies on conventional phenotypic methods which are time consuming. Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) is based on proteomic profiling and allows for rapid identification of pathogens.Objective: We compared MALDI-TOF MS against two commercial systems, MicroScan Walkaway and VITEK 2 MS.Methods: Over a three-month period from July 2013 to September 2013, a total of 227 bacteria and yeasts were collected from an academic microbiology laboratory (N = 121; 87 Gramnegatives, seven Gram-positives, 27 yeasts) and other laboratories (N = 106; 35 Gram-negatives, 34 Gram-positives, 37 yeasts). Sixty-five positive blood cultures were initially processed with Bruker Sepsityper kit for direct identification.Results: From the 65 blood culture bottles, four grew more than one bacterial pathogen and MALDI-TOF MS identified only one isolate. The blood cultures yielded 21 Gram-negatives, 43 Gram-positives and one Candida. There were 21 Escherirchia coli isolates which were reported by the MALDI-TOF MS as E. coli/Shigella. Of the total 292 isolates, discrepant results were found for one bacterial and three yeast isolates. Discrepant results were resolved by testing with the API system with MALDI-TOF MS showing 100% correlation.Conclusion: The MALDI-TOF MS proved to be very useful for rapid and reliable identification of bacteria and yeasts directly from blood cultures and after culture of other specimens. The difference in time to identification was significant for all isolates. However, for positive blood cultures with minimal sample preparation time there was a massive difference in turn-around time with great appreciation by clinicians.

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