scholarly journals Recent Progress in Endothelial Progenitor Cell Culture Systems: Potential for Stroke Therapy

2016 ◽  
Vol 56 (6) ◽  
pp. 302-309 ◽  
Author(s):  
Shunya TAKIZAWA ◽  
Eiichiro NAGATA ◽  
Taira NAKAYAMA ◽  
Haruchika MASUDA ◽  
Takayuki ASAHARA
Keyword(s):  
Cell Culture ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Recent Progress ◽  
Stroke Therapy ◽  
Endothelial Progenitor ◽  
Culture Systems ◽  
Cell Culture Systems

Recent Progress in Prediction Systems for Drug-induced Liver Injury Using in vitro Cell Culture

2020 ◽  
Vol 14 ◽  
Author(s):  
Shogo Ozawa ◽  
Toshitaka Miura ◽  
Jun Terashima ◽  
Wataru Habano ◽  
Seiichi Ishida
Keyword(s):  
Cell Culture ◽  
Recent Progress ◽  
Inflammatory Cells ◽  
Protein Adducts ◽  
In Vitro Assays ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Culture Systems ◽  
Cell Culture Systems

Background: In order to avoid drug-induced liver injury (DILI), in vitro assays, which enable the assessment of both metabolic activation and immune reaction processes that ultimately result in DILI, are needed. Objective: In this study, the recent progress in the application of in vitro assays using cell culture systems is reviewed for potential DILI-causing drugs/xenobiotics and a mechanistic study on DILI, as well as for the limitations of in vitro cell culture systems for DILI research. Methods: Information related to DILI was collected through a literature search of the PubMed database. Results: The initial biological event for the onset of DILI is the formation of cellular protein adducts after drugs have been metabolically activated by drug metabolizing enzymes. The damaged peptides derived from protein adducts lead to the activation of CD4+ helper T lymphocytes and recognition by CD8+ cytotoxic T lymphocytes, which destroy hepatocytes through immunological reactions. Because DILI is a major cause of drug attrition and drug withdrawal, numerous in vitro systems consisting of hepatocytes and immune/inflammatory cells, or spheroids of human primary hepatocytes containing non-parenchymal cells have been developed. These cellular-based systems have identified DILIinducing drugs with approximately 50% sensitivity and 90% specificity. Conclusion: Different co-culture systems consisting of human hepatocyte-derived cells and other immune/inflammatory cells have enabled the identification of DILI-causing drugs and of the actual mechanisms of action.

scholarly journals Effectiveness of endothelial progenitor cell culture under microgravity for improved angiogenic potential

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hiroko Hagiwara ◽  
Akira Higashibata ◽  
Shiho Ogawa ◽  
Shigeyuki Kanazawa ◽  
Hiroshi Mizuno ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Endothelial Progenitor ◽  
Angiogenic Potential

scholarly journals Swine endothelial progenitor cell culture

2015 ◽  
Vol 1 (4) ◽  
Author(s):  
Hong-Jian Shi ◽  
You-Hua Huang ◽  
Qian Xu ◽  
Tao Shen ◽  
Jian-Ke Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Endothelial Progenitor

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

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