scholarly journals c-3. Possibility of Application of Freege-cleave Fechnique in the Electron microscopic Study of the Brain

1968 ◽  
Vol 10 ◽  
pp. 96a-96a
Author(s):  
Kuniyuki SOMEDA ◽  
W. H. SWEET ◽  
R. S. WEINSTEIN
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
The Brain

Electron Microscopic Study of Freshly Fixed and Time-Delay Fixed Tissues in Rat

1975 ◽  
Vol 33 ◽  
pp. 618-619
Author(s):  
B. S. Lim ◽  
J. D. Solomon
Keyword(s):  
Time Delay ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Striated Muscle ◽  
Morphological Changes ◽  
Male Rat ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Pathology Laboratory ◽  
The Brain

In a pathology laboratory, many tissues which are submitted or made available for electron microscopic study are obtained from autopsy. Morphological changes due to postmortem delay which may not be visualized when studied with a light microscope are readily seen with the electron microscope. Because of this, it is important to make a comparative study of the freshly fixed and time-delay fixed tissues assimilating those obtained at autopsy. This work presents the results of morphological changes in the brain, heart, kidney, liver, and striated skeletal muscle of the rat with a postmortem time-delay of 24 hours. In the case of striated muscle the time delay was up to 48 hours before fixation.Methods and Materials A 62 day old male rat of the Sprague Dawley strain, weighing 410 grams was used in this experiment. The rat was anesthetized with nembutal and quickly opened. The brain, heart, liver, kidney, and a small piece of femoral muscle were quickly removed.

The interperiosteo-dural concept applied to the perisellar compartment: a microanatomical and electron microscopic study

2010 ◽  
Vol 113 (5) ◽  
pp. 1045-1052 ◽  
Author(s):  
Patrick François ◽  
Nadine Travers ◽  
Emmanuel Lescanne ◽  
Brigitte Arbeille ◽  
Michel Jan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Venous Blood ◽  
Electron Microscopic ◽  
Temporal Fossa ◽  
Optical Magnification ◽  
Superior Approach ◽  
Spinal Epidural ◽  
The Brain

Object The dura mater has 2 dural layers: the endosteal layer (outer layer), which is firmly attached to the bone, and the meningeal layer (inner layer), which directly covers the brain. These 2 dural layers join together in the middle temporal fossa or the convexity and separate into the orbital, lateral sellar compartment (LSC), or spinal epidural space to form the extradural neural axis compartment (EDNAC). The aim of this work was to anatomically verify the concept of the EDNAC by using electron microscopy. Methods The authors studied the cadaveric heads obtained from 13 adults. Ten of the specimens (or 20 perisellar areas) were injected with colored latex and fixed in formalin. They carefully removed each brain to allow a superior approach to the perisellar area. The 3 other specimens were studied by microscopic and ultrastructural methods to describe the EDNAC in the perisellar area. Special attention was paid to the dural layers surrounding the perisellar area. The authors studied the anatomy of the meningeal architecture of the LSC, the petroclival venous confluence, the orbit, and the trigeminal cave. After dissection, the authors took photographs of the dural layers with the aid of optical magnification. The 3 remaining heads, obtained from fresh cadavers, were prepared for electron microscopic study. Results The EDNAC is limited by the endosteal layer and the meningeal layer and contains fat and/or venous blood. The endosteal layer and meningeal layer were not identical on electron microscopy; this finding can be readily related to the histology of the meninges. Conclusions In this study, the authors demonstrated the existence of the EDNAC concept in the perisellar area by using dissected cadaveric heads and verified the reality of the concept of the meningeal layer with electron microscopy. These findings clearly demonstrated the existence of the EDNAC, a notion that has generally been accepted but never demonstrated microscopically.

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