Mapping Dynamic Lipid Biochemistry at Cellular Resolution Using Advanced Mass Spectrometry Imaging Technologies

2021 ◽  
Author(s):  
Shane Ellis
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Lipid Biochemistry ◽  
Imaging Technologies ◽  
Cellular Resolution

scholarly journals Approaching cellular resolution and reliable identification in mass spectrometry imaging of tryptic peptides

2018 ◽  
Vol 410 (23) ◽  
pp. 5825-5837 ◽  
Author(s):  
Katharina Huber ◽  
Pegah Khamehgir-Silz ◽  
Thorsten Schramm ◽  
Vladimir Gorshkov ◽  
Bernhard Spengler ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Tryptic Peptides ◽  
Reliable Identification ◽  
Cellular Resolution

TARGETED MASS spectrometry Imaging: Specific Targeting Mass Spectrometry imaging technologies from history to perspective

2012 ◽  
Vol 47 (3) ◽  
pp. 133-174 ◽  
Author(s):  
Hugo Gagnon ◽  
Julien Franck ◽  
Maxence Wisztorski ◽  
Robert Day ◽  
Isabelle Fournier ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Imaging Technologies ◽  
Specific Targeting

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

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