Two- and Three-dimensional Liquid Chromatography with Quadruple (x4) Parallel Mass Spectrometry for Triacylglycerol Analysis

2021 ◽  
Author(s):  
William Byrdwell
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Three Dimensional ◽  
Triacylglycerol Analysis

Liquid chromatography mass spectrometry based characterization of epitope configurations

2020 ◽  
Vol 12 (45) ◽  
pp. 5476-5484
Author(s):  
Maren Christin Stillesby Levernæs ◽  
Arelí Urtubia Moe ◽  
Sigurd Leinæs Bøe ◽  
Elisabeth Paus ◽  
Léon Reubsaet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Liquid Chromatography Mass Spectrometry ◽  
Three Dimensional Structure ◽  
Chromatography Mass Spectrometry ◽  
Pre Treatment

Here we evaluate a quick and easy tool for determination of epitope configuration using immunocapture and liquid chromatography mass spectrometry (LC-MS) subsequent to pre-treatment of the target protein to disrupt its three-dimensional structure.

scholarly journals Proteomic Profiling of Emiliania huxleyi Using a Three-Dimensional Separation Method Combined with Tandem Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3028
Author(s):  
Goyeun Yun ◽  
Jong-Moon Park ◽  
Van-An Duong ◽  
Jeong-Hun Mok ◽  
Jongho Jeon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Three Dimensional ◽  
Emiliania Huxleyi ◽  
Tandem Mass ◽  
Biological Processes ◽  
Proteomic Profiling ◽  
Wide Range ◽  
Molecular Functions

Emiliania huxleyi is one of the most abundant marine planktons, and it has a crucial feature in the carbon cycle. However, proteomic analyses of Emiliania huxleyi have not been done extensively. In this study, a three-dimensional liquid chromatography (3D-LC) system consisting of strong cation exchange, high- and low-pH reversed-phase liquid chromatography was established for in-depth proteomic profiling of Emiliania huxleyi. From tryptic proteome digest, 70 fractions were generated and analyzed using liquid chromatography-tandem mass spectrometry. In total, more than 84,000 unique peptides and 10,000 proteins groups were identified with a false discovery rate of ≤0.01. The physicochemical properties of the identified peptides were evaluated. Using ClueGO, approximately 700 gene ontology terms and 15 pathways were defined from the identified protein groups with p-value ≤0.05, covering a wide range of biological processes, cellular components, and molecular functions. Many biological processes associated with CO2 fixation, photosynthesis, biosynthesis, and metabolic process were identified. Various molecular functions relating to protein binding and enzyme activities were also found. The 3D-LC strategy is a powerful approach for comparative proteomic studies on Emiliania huxleyi to reveal changes in its protein level and related mechanism.

scholarly journals Top-Down and Intact Protein Mass Spectrometry Data Visualization for Proteoform Analysis Using VisioProt-MS

2019 ◽  
Vol 13 ◽  
pp. 117793221986822 ◽  
Author(s):  
Jean Lesne ◽  
Marie-Pierre Bousquet ◽  
Julien Marcoux ◽  
Marie Locard-Paulet
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Data Visualization ◽  
Web Application ◽  
Three Dimensional ◽  
Mass Spectrometry Data ◽  
Intact Protein ◽  
Top Down ◽  
Molecular Weights ◽  
Protein Mass

The rise of intact protein analysis by mass spectrometry (MS) was accompanied by an increasing need for flexible tools allowing data visualization and analysis. These include inspection of the deconvoluted molecular weights of the proteoforms eluted alongside liquid chromatography (LC) through their representation in three-dimensional (3D) liquid chromatography coupled to mass spectrometry (LC-MS) maps (plots of deconvoluted molecular weights, retention times, and intensity of the MS signal). With this aim, we developed a free and open-source web application named VisioProt-MS ( https://masstools.ipbs.fr/mstools/visioprot-ms/ ). VisioProt-MS is highly compatible with many algorithms and software developed by the community to integrate and deconvolute top-down and intact protein MS data. Its dynamic and user-friendly features greatly facilitate analysis through several graphical representations dedicated to MS and tandem mass spectrometry (MS/MS) analysis of proteoforms in complex samples. Here, we will illustrate the importance of LC-MS map visualization to optimize top-down acquisition/search parameters and analyze intact protein MS data. We will go through the main features of VisioProt-MS using the human proteasomal 20S core particle as a user-case.

Sign in / Sign up

Export Citation Format

Share Document