High frequency induction of multiple shoots and plant regeneration from zygotic embryo axis explants of cotton cultivar L-604 (Gossypium hirsutum L.).

2016 ◽  
Vol 5 (09) ◽  
pp. 4791
Author(s):  
Vijaya Naresh Juturu ◽  
Gopala Krishna Mekala ◽  
Akila Chandra Sekhar ◽  
Puli Chandra Obul Reddy ◽  
Mallikarjuna Garladinne*
Keyword(s):  
Gossypium Hirsutum ◽  
Zygotic Embryo ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Naphthalene Acetic Acid ◽  
Embryo Axis ◽  
Gossypium Hirsutum L ◽  
In Vitro Shoots ◽  
Elite Cultivar

An efficient, robust regeneration protocol has been developed using zygotic embryo axis explants of an elite cultivar L-604 (Gossypium hirsutum L.). Explants regenerated on MSB5 medium supplemented with 0.5 mg-l Thidiazuron, 0.1 mg-l Naphthalene acetic acid, 5.0 mg-l Silver nitrate and 1.0 g-l activated charcoal, was identified as the best combination for producing the maximum number (12.67) of multiple shoots per explant. In addition, the highest shoot elongation (5.30 cm) and maximum regeneration frequency (89.0%) was also recorded in the same hormonal combinations. In vitro shoots were successfully rooted on ½ MSB5 medium containing 1.5 mg-l IBA. Well rooted plants were subsequently transferred to polyhouse and greenhouse conditions for primary and secondary hardenings respectively and about 95% of the plants were established successfully. The conditions optimized in this study for inducing multiple shoots could be best suitable for development of transgenic L-604 variety for agronomically important traits.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals In vitro plant regeneration of Iraqi cotton (Gossypium hirsutum L.) cultivars through embryonic axis

2014 ◽  
Vol 8 (2) ◽  
pp. 90-94
Author(s):  
Hussein Abdullah Ahmed ◽  
Mortaza Hajyzadeh ◽  
Surendra Barpete ◽  
Sebahattin Ozcan
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Activated Charcoal ◽  
Embryonic Axis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Gossypium Hirsutum L ◽  
In Vitro Plant

In plant tissue culture studies, obtaining new plantlets from different parts of plant is a very important feature with direct or indirect ways of regeneration. The plant show different regeneration capacities from species to species. In this present study, in-vitro direct shoots development from embryonic axis of cotton (Gossypium hirsutum L. cv. Lashata) were comparatively studied. Embryonic axis, embryonic hypocotyl and plumule obtained from five days old in-vitro grown seeds. All explants were cultured on MS media supplemented with 1- 4 mg/l Benzyl amino purine (BAP) + 0.1 mg/l Naphthalene acetic acid (NAA) and 3 mg/l activated charcoal. Cultured explants were kept at growth chamber with photoperiod for 16 hours light and 8 hours dark, at 25oC. Reproducible in-vitro plant regeneration was obtained from embryonic axis when cultured onto MS medium supplemented 1 mg/l BAP + 0.1 mg/l NAA and 3 g/l Activated charcoal. Shoot induction was to be noted 53.3%, 36.7% and 12.3 in embryonic axis, plumule and embryonic hypocotyl respectively. All these shoots were capable of rooting on MS medium supplemented with 0.1 mg/l NAA, and establishing in soil 3-4 weeks.

In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)

1997 ◽  
Vol 16 (9) ◽  
pp. 647-652 ◽  
Author(s):  
D. C. Agrawal ◽  
A. K. Banerjee ◽  
R. R. Kolala ◽  
A. B. Dhage ◽  
A. V. Kulkarni ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Multiple Shoots ◽  
Gossypium Hirsutum L ◽  
In Vitro Induction

In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)

1997 ◽  
Vol 16 (9) ◽  
pp. 647-652
Author(s):  
D. C. Agrawal ◽  
A. K. Banerjee ◽  
R. R. Kolala ◽  
A. B. Dhage ◽  
A. V. Kulkarni ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Multiple Shoots ◽  
Gossypium Hirsutum L ◽  
In Vitro Induction

In Vitro Regeneration of Four Commercial Cotton Cultivars (Gossypium hirsutum L.) Grown in Xinjiang, China

2008 ◽  
Vol 34 (8) ◽  
pp. 1374-1380
Author(s):  
Tian-Zi CHEN ◽  
Shen-Jie WU ◽  
Fei-Fei LI ◽  
Wang-Zhen GUO ◽  
Tian-Zhen ZHANG
Keyword(s):  
Gossypium Hirsutum ◽  
In Vitro Regeneration ◽  
Gossypium Hirsutum L

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals HYDROALCOHOLIC EXTRACTION OF SHOOT CULTURES FROM NOTHAPODYTE NIMMONIANA AND ITS ANTI-PROLIFERATIVE ANALYSIS

2018 ◽  
Vol 10 (6) ◽  
pp. 32
Author(s):  
Arun Sharma ◽  
Ankita Rajata ◽  
Udayabanu Malairaman ◽  
Hemant Sood
Keyword(s):  
Hplc Analysis ◽  
Soxhlet Extraction ◽  
Multiple Shoots ◽  
Alcoholic Extract ◽  
Shoot Cultures ◽  
Old Field ◽  
Nothapodytes Nimmoniana ◽  
In Vitro Shoots ◽  
Ethidium Bromide Staining

Objective: The important restraints in plant-derived medicine are the convenience of active composites which depends on diverse topographical situations, active compound build-up pattern, environmental circumstances and genetic makeup of the plant. The presented work was aimed to describe an efficient technique for fast propagation of Nothapodytes nimmoniana which is an imperative source of Camptothecin.Methods: Multiple shoots were successfully regenerated from the explants N. nimmoniana using Murashige and Skoog (MS) medium accompanied with IBA+KN+GA3 (1:3:2) mg/l. the hydro-alcoholic extract was obtained by soxhlet extraction for field grown roots (fgRE), shoots (fgSE) and in vitro plantlets (ivPL) and further subjected to phytochemicals analysis. HPLC analysis was performed to determine the percentage of camptothecin (CAM). Anti-proliferative studies were carried out followed by Acridine orange/Ethidium bromide staining. Extracts were also estimated for reactive nitrogen species (RNS) and reactive oxygen species (ROS).Results: Preliminary qualitative chemical analysis of the extracts displayed the presence of alkaloids, carbohydrates, steroids, saponins, terpenoids and phenolics compounds. HPLC analysis revealed the presence of CAM in all the extracts. ivPL exhibited lowest anti-proliferative activity in contrast to other extract and percentage of ROS and RNS was also less. Although ivPL did not exhibited prominent anti-tumour effect, but in vitro accumulation of CAM in 2-months old shoots provides an alternative resource to 5 y-old field grown plants.Conclusion: This work delivers a potential to lead to further increase the potency of CAM in in vitro shoots of two months old as an alternative to the field grown plants for treating cancers.

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

Sign in / Sign up

Export Citation Format

Share Document