scholarly journals Proteomic study of salt tolerant cyanobacterium Anabaena sp. BHUAR002 isolated from Usar soil

2018 ◽  
Vol 7 (3) ◽  
pp. 2154
Author(s):  
Aparna Rai
Keyword(s):  
Salt Stress ◽  
Soluble Protein ◽  
Cytidine Deaminase ◽  
Protein Profile ◽  
Total Soluble Protein ◽  
Xanthomonas Oryzae Pv.Oryzae ◽  
Proteomic Study ◽  
Dna Replication And Repair ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp

Salt stress leads to an alteration in protein profile and induction of stress-specific proteins. The SDS – PAGE analysis of total soluble proteins of Anabaena sp. BHUAR002 (Accession no. bankit1353506 HM235817) exposed to 500 mM NaCl for 24 h revealed inhibition of host proteins, induction of selected proteins and appearance of some new proteins. In view of the appreciable alteration in total soluble protein profile after 500 mM salt treatment for 24 h, this dose was selected for further physiological, biochemical and proteomic analysis of the response of Anabaena sp. BHUAR002 to salinity and to examine the relationship between these responses. Further, 2DE of the total soluble protein of Anabaena sp. BHUAR002 showed 73 spots present only in control, 43 spots present only in stress and 15 differentially expressed spots present in both control and stress but show different levels of expression. This may be due to disturbance of cellular homeostasis by salt stress. Out of fifteen, Six spots were identified after MALDI-TOF MS analysis were identified as Manganese and iron Superoxide dismutase  [Anabaenavariabilis ATCC 29413], Cytidine deaminase [Porphyromonas venonis 60-3], Phycobilisome Protein [Nodularia Spumigena CCY9414], DNA replication and repair protein recF [Yersinia aldovae ATCC35236], IS1112 transposase [Xanthomonas oryzae pv.oryzae PXO99A] and TP901 family phage tail tape measure protein [Xanthobacter autotrophicus Py2].

scholarly journals Complete Genome Sequence of the Cyanobacterium Anabaena sp. 33047

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Sarah Pfeffer ◽  
R. Malcolm Brown
Keyword(s):  
Salt Stress ◽  
Nucleotide Sequence ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Nucleotide Sequence ◽  
Complete Genome ◽  
Marine Cyanobacterium ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Cyanobacterial Diversity

This study presents the complete nucleotide sequence of Anabaena sp. ATCC 33047 ( Anabaena CA), a filamentous, nitrogen-fixing marine cyanobacterium, which under salt stress conditions accumulates sucrose internally. The elucidation of the genome will contribute to the understanding of cyanobacterial diversity.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou
Keyword(s):  
Target Gene ◽  
Gene Inactivation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Tissue culture and genetic analysis of somaclonal variations of Solanum melongena L. cv. Nirrala

2014 ◽  
Vol 9 (12) ◽  
pp. 1182-1195
Author(s):  
Samar Naseer ◽  
Tariq Mahmood
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Soluble Protein ◽  
Solanum Melongena ◽  
Total Soluble Protein ◽  
Naphthalene Acetic Acid ◽  
Fresh Tissue ◽  
Somaclonal Variations ◽  
Random Amplification ◽  
Plant Grown

AbstractThe present study was designed to analyze genetically somaclonal variants using biochemical and molecular markers. Efficient tissue culture protocol for Solanum melongena L. cv. Nirrala was developed. Maximum callus induction (100%) was observed for Murashige and Skoog (MS) media supplemented with 2.0 mg L−1 naphthalene acetic acid +0.5 mg L−1 6-benzylaminopurine; and nodal explants gave best callusing response (88.8%) as compared to internodes (88.3%) and leaves (87.7%). The best shooting was induced on nodal and internodal callus in the presence of 2.0 mg L−1 6-benzylaminopurine. Total soluble protein content of callus and regenerated variant plants was estimated for biochemical analysis, and largest amount of soluble protein was found in callus (6.54 mg g−1 fresh tissue) followed by variant plant grown on 2.0 mg L−1 6-benzylaminopurine (5.96 mg g−1 fresh tissue). Random amplification of polymorphic DNA technique was done with five decamer primers (OPC1-OPC5) and maximum polymorphism was detected by OPC 2 (26.99%) among all samples, whereas nodal callus on media containing 1.0 mg L−1 naphthalene acetic acid +1.0 mg L−1 6-benzylaminopurine showed highest polymorphism producing 22 bands, out of which 8 bands were polymorphic. The study shows that this marker system can provide better evaluation of genetic variation induced by tissue culture.

scholarly journals Spatio-Temporal Gene Induction Systems in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

2017 ◽  
Vol 59 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Akiyoshi Higo ◽  
Atsuko Isu ◽  
Yuki Fukaya ◽  
Toru Hisabori
Keyword(s):  
Gene Induction ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Spatio Temporal ◽  
Pcc 7120
Sign in / Sign up

Export Citation Format

Share Document