scholarly journals Survival of Microbial consortium in granular formulations, degradation and release of microorganisms in soil

2016 ◽  
Vol 5 (05) ◽  
pp. 1348 ◽  
Author(s):  
Swapna G.* ◽  
Divya M. ◽  
Brahmaprakash G.P.
Keyword(s):  
Finger Millet ◽  
Microbial Consortium ◽  
Green Revolution ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Agriculture Sustainability ◽  
Plant Growth Promoting ◽  
Food Grain ◽  
Major Nutrients ◽  
Phosphate Solubilizing

The green revolution bought amazing consequences in food grain production but with insufficient concern for agriculture sustainability. Biofertilizers are gaining importance in sustaining agriculture. Various complementing combinations of microbial inoculants for management of major nutrients are necessary for agriculture sustainability. The present investigation was conducted to study the survivability of granular formulations containing Nitrogen fixing bacteria (Azotobacter chrococcum), phosphate solubilizing bacteria (Bacillus megaterium) and plant growth promoting bacteria (Pseudomonas fluorescens) in consortium prepared using different flour. Maximum survival of microbial consortium was observed in Soybean, followed by Soybean + Semolina and Rice inoculant formulations. Minimum survival of population was observed in Ragi + Semolina inoculant formulation. Wheat, wheat + semolina, soybean, soybean + semolina granular formulations have shown better degradation compared to other granular formulations both in presence and absence of tomato and finger millet (Eleusine coracana). Among different granular inoculant formulations, Maximum release was observed in soybean granular inoculant formulations in presence of tomato and Finger millet plant and in absence of plant and minimum release of microbial consortium was observed in ragi + semolina granular inoculant formulations during incubation in soil.

scholarly journals Mineral Phosphate Solubilizing Bacteria Isolated from Various Plant Rhizosphere under Different Aluminum Content

2015 ◽  
Vol 10 (2) ◽  
Author(s):  
Dolly Iriani Damarjaya ◽  
Jaka Widada ◽  
Keishi Senoo ◽  
Masaya Nishiyama ◽  
Shigeto Otsuka
Keyword(s):  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Gene Sequence Analysis ◽  
Mineral Phosphate ◽  
Plant Growth Promoting ◽  
The Media ◽  
Formation Method ◽  
Phosphate Solubilizing

The objectives of this study was to isolate and characterize the mineral phosphate solubilizing bacteriafrom rhizosphere and evaluate their potential as plant growth promoting bacteria in Al-toxic soils. The halozone formation method was used to isolate PSB using the media containing insoluble phosphates (Ca-P or Al-P)as a source of phosphate. Eight of acid and Al-tolerant PSB isolates that were able to solubilize Ca-P wereobtained from rhizosphere of clover, wheat, corn, and sunflower grown in Al-toxic soil. Identification of theisolates based on the 16S rRNA gene sequence analysis demonstrated that the isolates were strains of Burkholderia(5 strains), Pseudomonas (1 strain), Ralstonia (1 strain), and unidentified bacterium (1 strains). All PSB isolatesshowed the capability to dissolve Ca-P, and only 1 strain (Ralstonia strain) was able to dissolve Al-P in agar platemedium. The P-solubilization by these isolates was correlated with pH of medium. Inoculation of the bacterialstrains on clover on Al-toxic medium showed that all isolates increased the plant dry weight compared withuninoculated treatment. Our results showed that those PSB isolates have potential to be developed as a biofertilizerto increase the efficiency of P-inorganic fertilizer used in Al-toxic soils.

scholarly journals Establishing and Quantifying the Phosphate Solubilizing Potential of the Brinjal Bacterial Isolate

2021 ◽  
Vol 10 (11) ◽  
pp. 83-88
Author(s):  
C. Jenifer Lolita A. C. Manjula ◽  
E. Keshamma
Keyword(s):  
Plant Growth ◽  
Bacterial Isolate ◽  
Nitrogenase Activity ◽  
Solanum Melongena ◽  
Steady Increase ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Beneficial Effects ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing

Rhizosphere, phylloplane and caulosphere is the region where a complex community of microbes, mainly bacteria and fungi are present. The microbe plant interaction in these regions can be beneficial, neutral, variable, or deleterious for plant growth. The bacteria that exert beneficial effects on plant development are termed plant growth promoting bacteria. To quantify the amount of phosphate solubilizing bacteria from rhizosphere, phylloplane and caulosphere of brinjal (Solanum melongena L.). Materials and methods: Brinjal (Solanum melongena L.) plants of different varieties were collected from seven locations around Bangalore viz., Hessaraghatta, Yelahanka, Kengeri, Madi vala, Hebbal, Tirumalapura and Attibele were also screened for the presence of phosphate solubilizing bacteria. Nitrogenase activity was estimated by acetylene reduction assay and analyzed by gas chromatography. The amount of nitrogen fixed brinjal bacterial isolate was quantified by micro Kjeldhal method. The amount of nitrogen fixed by the BBI was equivalent to 23.5 nm of C2H2 reduced/tube/hour. The amount of nitrogen fixed by the BBI showed a steady increase upto three days (75 nm of C2H2 reduced/tube/hour) after which there was a decline in the amount of nitrogen fixed by the microbe. Phosphate solubilization by the bacteria isolated from brinjal is highly beneficial to the crop, as it would always make more phosphate available to the crop. This phosphate solubilizing potential could be harnessed to reduce the input of inorganic fertilizers. For the first time the presence of phosphate solubilizing bacteria on the rhizosphere and endorhizosphere of brinjal (Solanum Melongena L.) cultivars was established.

scholarly journals Profiling of Plant Growth-Promoting Metabolites by Phosphate-Solubilizing Bacteria in Maize Rhizosphere

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1071
Author(s):  
Minchong Shen ◽  
Jiangang Li ◽  
Yuanhua Dong ◽  
Hong Liu ◽  
Junwei Peng ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Control ◽  
Agricultural Practices ◽  
Phosphate Solubilizing Bacteria ◽  
Mean Values ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Microbial Treatments

Microbial treatment has recently been attracting attention as a sustainable agricultural strategy addressing the current problems caused by unreasonable agricultural practices. However, the mechanism through which microbial inoculants promote plant growth is not well understood. In this study, two phosphate-solubilizing bacteria (PSB) were screened, and their growth-promoting abilities were explored. At day 7 (D7), the lengths of the root and sprout with three microbial treatments, M16, M44, and the combination of M16 and M44 (Com), were significantly greater than those with the non-microbial control, with mean values of 9.08 and 4.73, 7.15 and 4.83, and 13.98 and 5.68 cm, respectively. At day 14 (D14), M16, M44, and Com significantly increased not only the length of the root and sprout but also the underground and aboveground biomass. Differential metabolites were identified, and various amino acids, amino acid derivatives, and other plant growth-regulating molecules were significantly enhanced by the three microbial treatments. The profiling of key metabolites associated with plant growth in different microbial treatments showed consistent results with their performances in the germination experiment, which revealed the metabolic mechanism of plant growth-promoting processes mediated by screened PSB. This study provides a theoretical basis for the application of PSB in sustainable agriculture.

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

scholarly journals Multi-Omics Reveal the Efficient Phosphate-Solubilizing Mechanism of Bacteria on Rocky Soil

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanqiang Ding ◽  
Zhuolin Yi ◽  
Yang Fang ◽  
Sulan He ◽  
Yuming Li ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Agricultural Development ◽  
Tricarboxylic Acid Cycle ◽  
Acid Synthesis ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Fertilizers ◽  
Plant Growth Promoting ◽  
Rocky Soil ◽  
Phosphate Solubilizing

Phosphate-solubilizing bacteria (PSB) can alleviate available phosphorus (AP)-deficiency without causing environmental pollution like chemical phosphate fertilizers. However, the research and application of PSB on the barren rocky soil is very rare. We screened six PSB from sweetpotato rhizosphere rocky soil. Among them, Ochrobactrum haematophilum FP12 showed the highest P-solubilizing ability of 1,085.00 mg/L at 7 days, which was higher than that of the most reported PSB. The assembled genome of PSB FP12 was 4.92 Mb with P-solubilizing and plant growth-promoting genes. In an AP-deficient environment, according to transcriptome and metabolomics analysis, PSB FP12 upregulated genes involved in gluconic acid synthesis and the tricarboxylic acid cycle, and increased the concentration of gluconic acid and malic acid, which would result in the enhanced P-solubilizing ability. Moreover, a series of experiments in the laboratory and field confirmed the efficient role of the screened PSB on significantly increasing AP in the barren rocky soil and promoting sweetpotato yield. So, in this study, we screened highly efficient PSB, especially suitable for the barren rocky soil, and explored the P-solubilizing mechanism. The research will reduce the demand for chemical phosphate fertilizers and promote the environment-friendly agricultural development.

scholarly journals A Bacterium Isolated From Soil in a Karst Rocky Desertification Region Has Efficient Phosphate-Solubilizing and Plant Growth-Promoting Ability

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinge Xie ◽  
Zongqiang Yan ◽  
Guifen Wang ◽  
Wenzhi Xue ◽  
Cong Li ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gluconic Acid ◽  
Lateral Roots ◽  
Utilization Efficiency ◽  
Phosphate Solubilizing Bacteria ◽  
Karst Rocky Desertification ◽  
Rocky Desertification ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Phosphorus in the soil accessible to plants can easily be combined with calcium ion, the content of which is high in karst rocky desertification (KRD) regions, thereby resulting in a low utilization efficiency of phosphorus. The application of phosphate-solubilizing bacteria (PSB) from the KRD region would facilitate enhanced phosphate availability in the soil. In the present study, the strains belonging to Acinetobacter, Paraburkholderia, and Pseudomonas with efficient phosphate-solubilizing ability were isolated from fruit tree rhizosphere soils in KRD regions. Particularly, Acinetobacter sp. Ac-14 had a sustained and stable phosphate-solubilizing ability (439–448 mg/L, 48–120 h). Calcium carbonate decreased the phosphate-solubilizing ability in liquid medium; however, it did not affect the solubilization index in agar-solidified medium. When cocultivated with Arabidopsis thaliana seedling, Ac-14 increased the number of lateral roots, fresh weight, and chlorophyll content of the seedlings. Metabolomics analysis revealed that Ac-14 could produce 23 types of organic acids, majorly including gluconic acid and D-(-)-quinic acid. Expression of Ac-14 glucose dehydrogenase gene (gcd) conferred Pseudomonas sp. Ps-12 with a sustained and stable phosphate-solubilizing ability, suggesting that the production of gluconic acid is an important mechanism that confers phosphate solubilization in bacteria. Moreover, Ac-14 could also produce indole acetic acid and ammonia. Collectively, the isolated Ac-14 from KRD regions possess an efficient phosphate-solubilizing ability and plant growth-promoting effect which could be exploited for enhancing phosphorus availability in KRD regions. This study holds significance for the improvement of soil fertility and agricultural sustainable development in phosphorus-deficient KRD regions.

scholarly journals Enhanced Biogas and Biofertilizer Production from Anaerobic Codigestion of Harvest Residues and Goat Manure

2020 ◽  
pp. 1-13
Author(s):  
Uduak U. Ndubuisi-Nnaji ◽  
Utibe A. Ofon ◽  
Naomi U. Asamudo ◽  
Victoria M. Ekong
Keyword(s):  
Rice Straw ◽  
Production Efficiency ◽  
Fold Increase ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Populations ◽  
Harvest Residues ◽  
Biogas Yield ◽  
Plant Growth Promoting ◽  
Goat Manure ◽  
Phosphate Solubilizing

Comparative assays were conducted to assess the biogas and biofertilizer production efficiency from anaerobically codigested goat manure (GM) and harvest residues: corn stover (CS) and rice straw (RS). All digesters were operated simultaneously under mesophilic temperature of 40°C and notable phosphate solubilizing and nitrogen fixing bacterial populations indicated qualitative biofertilizer quality of the digestates. Codigestion of the substrates significantly increased biogas yield (p < 0.05) compared to monodigestion, and the highest cumulative yield of 573 ml/g VS was obtained from codigested rice straw (RS) and goat manure (GM). With a significant decimation in number of pathogens (p < 0.05), a 2 – 3 fold increase in populations of plant growth promoting bacteria (Bacillus and Pseudomonas species) was observed in digestate from codigestion assays when compared to monodigestion (control) and were identified as Clostridium sp., Bacillus subtilis, Bacillus megaterium, Lactobacillus sp., Pseudomonas fluorescens including methanogens: Methanothrix sp., Methanobacterium sp. and Methanosarcina sp. On the average, codigestion assays resulted in enhanced biogas yield and biofertilizer quality that was 2.3 – 4.1 times higher than single substrate digestion and generally improved the efficiency of biogas and biofertilizer production.

Production of Plant Growth Promoting Substances by Phosphate Solubilizing Bacteria Isolated from Vertisols

2007 ◽  
Vol 2 (3) ◽  
pp. 326-333 ◽  
Author(s):  
A. Vikram ◽  
H. Hamzehzarghani . ◽  
A.R. Alagawadi . ◽  
P.U. Krishnaraj . ◽  
B.S. Chandrashekar .
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilizing Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing
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