Application of a Convective-Dispersion Model to Predict In Vivo Hepatic Clearance from In Vitro Measurements Utilizing Cryopreserved Human Hepatocytes

2003 ◽  
Vol 4 (5) ◽  
pp. 357-369 ◽  
Author(s):  
Ryan Niro ◽  
James Byers ◽  
Ronald Fournier ◽  
Kenneth Bachmann
Keyword(s):  
Dispersion Model ◽  
Hepatic Clearance ◽  
Human Hepatocytes ◽  
Convective Dispersion ◽  
In Vitro Measurements

scholarly journals Alcohol-and-HIV-Induced Lysosomal Dysfunction Regulates Extracellular Vesicles Secretion in Vitro and in Liver-Humanized Mice

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 29
Author(s):  
Raghubendra Singh Dagur ◽  
Moses New-Aaron ◽  
Murali Ganesan ◽  
Weimin Wang ◽  
Svetlana Romanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Extracellular Vesicles ◽  
Treated Group ◽  
Human Hepatocytes ◽  
In Vivo Studies ◽  
People Living With Hiv ◽  
Humanized Mouse Model ◽  
And Function

Background: Alcohol abuse is common in people living with HIV-1 and dramaticallyenhances the severity of HIV-induced liver damage by inducing oxidative stress and lysosomaldysfunction in the liver cells. We hypothesize that the increased release of extracellular vesicles(EVs) in hepatocytes and liver humanized mouse model is linked to lysosome dysfunction. Methods:The study was performed on primary human hepatocytes and human hepatoma RLWXP-GFP (Huh7.5 cells stably transfected with CYP2E1 and XPack-GFP) cells and validated on ethanol-fed liverhumanizedfumarylacetoacetate hydrolase (Fah)-/-, Rag2-/-, common cytokine receptor gamma chainknockout (FRG-KO) mice. Cells and mice were infected with HIV-1ADA virus. Results: We observedan increase in the secretion of EVs associated with a decrease in lysosomal activity and expressionof lysosomal-associated membrane protein 1. Next-generation RNA sequencing of primary humanhepatocytes revealed 63 differentially expressed genes, with 13 downregulated and 50 upregulatedgenes in the alcohol–HIV-treated group. Upstream regulator analysis of differentially expressedgenes through Ingenuity Pathway Analysis identified transcriptional regulators affecting downstreamgenes associated with increased oxidative stress, lysosomal associated disease, and function andEVs biogenesis. Our in vitro findings were corroborated by in vivo studies on human hepatocytetransplantedhumanized mice, indicating that intensive EVs’ generation by human hepatocytes andtheir secretion to serum was associated with increased oxidative stress and reduction in lysosomalactivities triggered by HIV infection and ethanol diet. Conclusion: HIV-and-ethanol-metabolisminducedEVs release is tightly controlled by lysosome status in hepatocytes and participates in thedevelopment of double-insult-induced liver injury.

Correlation of in vivo and in vitro measurements of sun protection factor

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
M.-T. Sheu ◽  
C.-W. Lin ◽  
M.-C. Huang ◽  
C.-H. Shen ◽  
H.-O. Ho
Keyword(s):  
Sun Protection ◽  
Protection Factor ◽  
In Vitro Measurements ◽  
Sun Protection Factor

scholarly journals Advances in Photoacoustic Noninvasive Glucose Testing

1999 ◽  
Vol 45 (9) ◽  
pp. 1587-1595 ◽  
Author(s):  
Hugh A MacKenzie ◽  
Helen S Ashton ◽  
Stephen Spiers ◽  
Yaochun Shen ◽  
Scott S Freeborn ◽  
...  
Keyword(s):  
Glucose Measurement ◽  
Oral Glucose ◽  
Physiological Factors ◽  
Glucose Testing ◽  
In Vivo Experiments ◽  
In Vitro Measurements ◽  
Clinical Measurements ◽  
Blood Analytes

Abstract We report here on in vitro and in vivo experiments that are intended to explore the feasibility of photoacoustic spectroscopy as a tool for the noninvasive measurement of blood glucose. The in vivo results from oral glucose tests on eight subjects showed good correlation with clinical measurements but indicated that physiological factors and person-to-person variability are important. In vitro measurements showed that the sensitivity of the glucose measurement is unaffected by the presence of common blood analytes but that there can be substantial shifts in baseline values. The results indicate the need for spectroscopic data to develop algorithms for the detection of glucose in the presence of other analytes.

scholarly journals Intraperitoneal pressure: Stability over time and validation of Durand’s measurement method

2020 ◽  
pp. 089686082097312
Author(s):  
Alicia Sobrino-Pérez ◽  
Alfonso Pérez-Escudero ◽  
Lucila Fernández-Arroyo ◽  
Ana Dorado-García ◽  
Berta Martín-Alcón ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Venous Pressure ◽  
Relevant Parameter ◽  
Pressure System ◽  
Intraperitoneal Pressure ◽  
In Vitro Measurements ◽  
Long Timescales ◽  
Over Time

Intraperitoneal pressure (IPP) is gaining consideration as a relevant parameter of peritoneal dialysis (PD) in adults, although many of its aspects are still pending clarification. We address here its stability over time and the validity of the usual method of clinical measurement, as proposed by Durand in 1992 but never specifically validated. We performed this validation by comparing Durand’s method and direct measurements with a central venous pressure system. We performed a total of 250 measurement pairs in 50 patients with different intraperitoneal volumes plus in-vitro measurements with a simulated peritoneum. Absolute differences between the two systems in vivo were 0.87 ± 0.91 cmH2O (range 0–5 cmH2O); only 6.4% of them were ≥3 cmH2O. In vitro results for both methods were identical. We also compared IPP measurements in the same patient separated by 1–4 h (514 measurement pairs in 136 patients), 1 week (92 pairs in 92 patients), and 2 years (34 pairs in 17 patients). Net differences of measurements separated by hours or 1 week were close to 0 cmH2O, with oscillations of 1.5 cmH2O in hours and 2.3 cmH2O in 1 week. IPP measured 2 years apart presented a net decrease of 2.5 ± 4.9 cmH2O, without correlation with body mass index changes or any other usual parameter of PD. In hours, 7% of IPP differences were >3 cmH2O, 22% in 1 week, and 50% in 2 years. In conclusion, Durand’s method is precise enough to measure IPP in peritoneal dialysis. This parameter is not stable over long timescales, so it is necessary to use recent measurements.

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