scholarly journals Innovative Protocols for in SITU MTBE Degradation by Using Molecular Probes-An Enhanced Chemical-Bio Oxidation Technique

2009 ◽  
Author(s):  
Paul Fallgren
Keyword(s):  
Molecular Probes ◽  
Mtbe Degradation

scholarly journals Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2106
Author(s):  
Barbara Kij-Mitka ◽  
Halina Cernohorska ◽  
Svatava Kubickova ◽  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Molecular Cytogenetics ◽  
Molecular Probes ◽  
Domestic Cat ◽  
Fish Technique ◽  
Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

Detection of transcription and translation in situ with biotinylated molecular probes in cells transfected with recombinant DNA plasmids

1986 ◽  
Vol 156 (1) ◽  
pp. 17-24 ◽  
Author(s):  
G.H. Smith ◽  
P.J. Doherty ◽  
R.B. Stead ◽  
C.M. Gorman ◽  
D.E. Graham ◽  
...  
Keyword(s):  
Recombinant Dna ◽  
Molecular Probes ◽  
In Cells

Recent advances in stimuli-responsive in situ self-assembly of small molecule probes for in vivo imaging of enzymatic activity

2021 ◽  
Author(s):  
Yuqi Wang ◽  
Jianhui Weng ◽  
Xidan Wen ◽  
Yuxuan Hu ◽  
Deju Ye
Keyword(s):  
Enzymatic Activity ◽  
Small Molecule ◽  
Self Assembly ◽  
In Vivo Imaging ◽  
Molecular Probes ◽  
Stimuli Responsive ◽  
Small Molecule Probes ◽  
Recent Advances

Stimuli-responsive in situ self-assembly of small molecule probes into nanostructures has been promising for the construction of molecular probes for in vivo imaging.

scholarly journals Naturally Occurring Bacteria Similar to the Methyl tert-Butyl Ether (MTBE)-Degrading Strain PM1 Are Present in MTBE-Contaminated Groundwater

2003 ◽  
Vol 69 (5) ◽  
pp. 2616-2623 ◽  
Author(s):  
Krassimira Hristova ◽  
Binyam Gebreyesus ◽  
Douglas Mackay ◽  
Kate M. Scow
Keyword(s):  
Dna Sequences ◽  
Field Studies ◽  
Methyl Tert Butyl Ether ◽  
Butyl Ether ◽  
Naturally Occurring ◽  
Degrading Bacteria ◽  
Pure Cultures ◽  
Air Force Base ◽  
Mtbe Degradation

ABSTRACT Methyl tert-butyl ether (MTBE) is a widespread groundwater contaminant that does not respond well to conventional treatment technologies. Growing evidence indicates that microbial communities indigenous to groundwater can degrade MTBE under aerobic and anaerobic conditions. Although pure cultures of microorganisms able to degrade or cometabolize MTBE have been reported, to date the specific organisms responsible for MTBE degradation in various field studies have not be identified. We report that DNA sequences almost identical (99% homology) to those of strain PM1, originally isolated from a biofilter in southern California, are naturally occurring in an MTBE-polluted aquifer in Vandenberg Air Force Base (VAFB), Lompoc, California. Cell densities of native PM1 (measured by TaqMan quantitative PCR) in VAFB groundwater samples ranged from below the detection limit (in anaerobic sites) to 103 to 104 cells/ml (in oxygen-amended sites). In groundwater from anaerobic or aerobic sites incubated in microcosms spiked with 10 μg of MTBE/liter, densities of native PM1 increased to approximately 105 cells/ml. Native PM1 densities also increased during incubation of VAFB sediments during MTBE degradation. In controlled field plots amended with oxygen, artificially increasing the MTBE concentration was followed by an increase in the in situ native PM1 cell density. This is the first reported relationship between in situ MTBE biodegradation and densities of MTBE-degrading bacteria by quantitative molecular methods.

Polyclonal hematopoiesis with variable telomere shortening in human long-term allogeneic marrow graft recipients

Blood ◽  
2000 ◽  
Vol 96 (12) ◽  
pp. 3991-3994 ◽  
Author(s):  
George Mathioudakis ◽  
Rainer Storb ◽  
Peter A. McSweeney ◽  
Beverly Torok-Storb ◽  
Peter M. Lansdorp ◽  
...  
Keyword(s):  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Molecular Probes ◽  
Proliferative Potential ◽  
Marrow Graft ◽  
Hematopoietic Reconstitution ◽  
Complete Blood Counts ◽  
Allogeneic Marrow

Donor-derived hematopoiesis was assessed in 17 patients who received allogeneic marrow grafts from HLA-matched siblings between 1971 and 1980. Complete blood counts were normal or near normal in all patients except one. Chimerism analyses, using either dual-color XY-chromosome fluorescence in situ hybridization (FISH) or analysis of variable number tandem repeat loci, indicated that 15 out of 16 patients had greater than 97% donor-derived hematopoiesis, whereas 1 patient had indeterminate chimerism. All 12 recipients of grafts from female donors exhibited polyclonal hematopoiesis by X-linked clonal analysis with the use of molecular probes. Of the 17 recipients, 9 exhibited a less than 1.0-kilobase shortening of granulocyte telomere length compared with their respective donors, according to terminal restriction fragment analysis or flow-FISH with a fluorescein-labeled peptide nucleic acid probe. These data suggest that under standard transplantation conditions, the stem cell proliferative potential is not compromised during hematopoietic reconstitution.

scholarly journals All Three Promoters of the Acetyl-Coenzyme A-Carboxylase α-encoding Gene Are Expressed in Mammary Epithelial Cells of Ruminants

2003 ◽  
Vol 51 (8) ◽  
pp. 1073-1081 ◽  
Author(s):  
Adrian Molenaar ◽  
Jianqiang Mao ◽  
Kim Oden ◽  
Hans-Martin Seyfert
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
De Novo ◽  
Mammary Epithelial Cells ◽  
Molecular Probes ◽  
Mammary Epithelial ◽  
Encoding Gene ◽  
Rate Limiting ◽  
Acetyl Coenzyme A Carboxylase

The activity of the enzyme acetyl-CoA-carboxylase α (ACC-α) is rate limiting for the de novo synthesis of fatty acids. The encoding gene is expressed from three promoters in ruminants (PI-PIII). Their individual contribution to the formation of milk fat is unknown. Promoter-specific molecular probes were hybridized in situ to serial sections of mammary glands from cows and sheep to determine their developmental and spatial expression profile in the udder. We show that all three promoters are active in mammary epithelial cells (MECs) of udders from both species. This implies that, in principle, none of these promoters can be singled out as the key element controlling the ACC-α-related contribution to establishment of milk fat content, although the activity of PIII only is known to be disproportionally stimulated by lactation in MECs. We propose that all three promoters may be relevant for milk fat synthesis in cattle, whereas PII and PIII are crucial for milk fat formation in sheep. We show also that ACC-α synthesis is not strictly coupled to casein synthesis, particularly during pregnancy and involution.

Use of molecular probes to detect human cytomegalovirus and human immunodeficiency virus.

1989 ◽  
Vol 35 (8) ◽  
pp. 1581-1587 ◽  
Author(s):  
S A Spector ◽  
K Hsia ◽  
F Denaro ◽  
D H Spector
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Cytomegalovirus ◽  
Severe Disease ◽  
Effective Means ◽  
Molecular Probes ◽  
Treatment And Prevention ◽  
Immunodeficiency Virus ◽  
Polymerase Chain ◽  
A New Technique

Abstract Human cytomegalovirus (HCMV) and human immunodeficiency virus (HIV) cause severe disease. The identification of these viruses in clinical specimens and understanding the progression of infection and diseases relating to HCMV and HIV are essential to develop effective means for treatment and prevention. Here we describe the application of molecular probes to the diagnosis and pathogenesis of HCMV and HIV. In situ hybridization and the amplification procedure of polymerase chain reaction are used to detect both viruses; these techniques have provided important information regarding the pathogenesis of HCMV and HIV. A new technique, target cycling, may also prove useful for the detection of viruses by enriching for target sequences. The continued application of molecular probes to pathogenetic studies of HCMV and HIV promises to further our knowledge of these viruses, and of their interaction.

Phylogenetic analysis and in situ identification of “Nostocoida limicola”-like filamentous bacteria in activated sludge from industrial wastewater treatment plants

2002 ◽  
Vol 46 (1-2) ◽  
pp. 99-104 ◽  
Author(s):  
J. Snaidr ◽  
C. Beimfohr ◽  
C. Levantesi ◽  
S. Rossetti ◽  
J. van der Waarde ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Industrial Wastewater ◽  
Wastewater Treatment Plants ◽  
Molecular Probes ◽  
Special Focus ◽  
Filamentous Bacteria ◽  
Industrial Wastewater Treatment ◽  
Biological Methods

The diversity of filamentous bacteria present in industrial wastewater treatment plants was analysed by a combination of classical and molecular-biological approaches. Many unknown filamentous bacteria were observed in about 80 screened activated sludge samples from different industries with sometimes severe bulking sludge problems. A special focus was paid to filaments which resembled “Nostocoida limicola”, a filamentous bacterium which was found to be present in many WWTPs. These filamentous bacteria are hardly cultivable and only one strain was obtained and maintained in co-culture with a yeast. The 16S rRNA sequences of several other “Nostocoida limicola”-like filamentous bacteria from different sludge samples were obtained by micromanipulation and different molecular-biological methods. The sequences were phylogenetically analyzed and specific molecular probes were developed and applied. The results clearly demonstrate that “Nostocoida limicola”-like filaments from industrial WWTPs are different from all other “Nostocoida limicola” types investigated so far. Our strains are affiliated to the alpha-subclass of Proteobacteria.

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