scholarly journals Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria

2005 ◽  
Author(s):  
Salma Rahman
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Analytical Approaches

Recent progress on microfluidic biosensors for rapid detection of pathogenic bacteria

2021 ◽  
Author(s):  
Gaowa Xing ◽  
Weifei Zhang ◽  
Nan Li ◽  
Qiaosheng Pu ◽  
Jin-Ming Lin
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Recent Progress

Development of multiplex PCR assay for rapid detection Listeria monocytogenes in clinical samples

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Vulnerable Groups ◽  
Clinical Samples ◽  
Specific Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.

scholarly journals Using millimeter‐waves for rapid detection of pathogenic bacteria in food based on bacteriophage

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Kamel S. Sultan ◽  
Tamer A. Ali ◽  
Nada A. Fahmy ◽  
Ayman El‐Shibiny
Keyword(s):  
Millimeter Waves ◽  
Rapid Detection ◽  
Pathogenic Bacteria

scholarly journals Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

2017 ◽  
Vol 16 (6) ◽  
pp. 1188-1205 ◽  
Author(s):  
Diana C. Vanegas ◽  
Carmen L. Gomes ◽  
Nicholas D. Cavallaro ◽  
Daniel Giraldo-Escobar ◽  
Eric S. McLamore
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria

scholarly journals Rapid Detection of Pathogenic Bacteria in Whole Blood Samples Using 23S rRNA PCR Assays

2019 ◽  
Vol 13 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Farzaneh Firoozeh ◽  
Azam Shiralinezhad ◽  
Mansooreh Momen-Heravi ◽  
Esmat Aghadavod ◽  
Mohammad Zibaei
Keyword(s):  
Blood Culture ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Culture Technique ◽  
University Hospital ◽  
23S Rrna ◽  
Microbial Culture ◽  
Cross Sectional ◽  
Blood Samples ◽  
Pcr Assays

Purpose:Bloodstream infections are a general cause of death among hospitalized patients. Rapid diagnosis and timely treatment can reduce mortality. The aim of this investigation was to evaluate the 23S rRNA PCR assays as a rapid detection method for diagnose of sepsis in patients with suspected bacteremia.Methods:A cross-sectional study was conducted at Shahid Beheshti University Hospital in Kashan from November 2017 to December 2018. The blood samples of 265 patients with suspected bacteremia were studied by blood culture and 23S rRNA PCR techniques. The results were analyzed using SPSS version 16 and Chi-square test.Results:Eighty (30.2%) blood samples of 265 suspected patients, were identified as positive by PCR assays, whereas 27 (10.2%) were identified as positive by the blood culture technique. The statistical analysis showed a significant association between the results of PCR assays and blood culture and factors such as prior antibiotic use and underlying diseases (P˂0.05). Also a significant correlation was observed between laboratory and clinical criteria and the results of both PCR assays and blood culture (P˂ 0.05).Conclusion:The 23S rRNA PCR method is a rapid and sensitive technique specially for diagnosing sepsis among patients in whom bacteremia is difficult to diagnose with culture method including neonates and patients who have taken antibiotics before microbial culture.

Different Methods for Molecular and Rapid Detection of Human Novel Coronavirus

2021 ◽  
Vol 27 ◽  
Author(s):  
Mohamad Hesam Shahrajabian ◽  
Wenli Sun ◽  
Qi Cheng
Keyword(s):  
Nucleic Acid ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Scientific Information ◽  
Meat Products ◽  
Lamp Assay ◽  
Dna Amplification ◽  
Digital Pcr ◽  
Recombinase Polymerase Amplification ◽  
Nucleic Acid Amplification

Introduction: While PCR has been recognized as one of the appropriate ways to diagnosis of infectious diseases, Loop-mediated isothermal amplification (LAMP), which is a nucleic acid amplification method, can be considered as an alternative to PCR, and it is faster, cost-effective, and easier to perform than nested PCR. Patients and Methods: Keywords were searched in PubMed/MEDLINE, Scopus and Institute for Scientific Information Web of Science, as well as the search engine of Google Scholar. Keywords were PCR, LAMP, RAA, RPA, Virus and COVID-19. Results: LAMP technology has been extensively applied for the detection of human pathogenic bacteria, crop pests, pathogenic organisms and components in meat products. A new isotheral method, Recombinase polymerase amplification (RPA), can amplify the DNA as well as RPA. RPA has benefited from isothermal PCR and both simplicity and rapid amplification. Recombinase aided amplification (RAA) assay has been favorably used in the detection of bacterial and viral pathogens and solved the technical difficulties posed by DNA amplification methods because it does not need thermal denaturation of the template and employs at a low and constant temperature. Conclusions: Reverse transcription polymerase chain reaction, digital PCR, LAMP, nicking endonuclease amplification reaction, recombinase polymerase amplification, and clustered regularly interspaced short palindromic repeats are different nucleic acid amplification tests of COVID-19. LAMP methods can be more specific than qPCR and immunoassays. The LAMP assay can be applied for rapid detection of SARS-CoV, MERS-CoV, SARS-CoV-2, and influenza, because LAMP is a highly sensitive and specific DNA/RNA amplification technique.

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