scholarly journals Deployment Plan for Bioremediation and Natural Attenuation for In Situ Restoration of Chloroethene-Contaminated Groundwater

1999 ◽  
Author(s):  
L.N. Peterson ◽  
R.C. Starr ◽  
K.S. Sorenson ◽  
R.W. Smith ◽  
T.J. Phelps
Keyword(s):  
Natural Attenuation ◽  
Contaminated Groundwater

Robustness of an aerobic metabolically vinyl chloride degrading bacterial enrichment culture

2011 ◽  
Vol 64 (9) ◽  
pp. 1796-1803 ◽  
Author(s):  
He-Ping Zhao ◽  
Kathrin R. Schmidt ◽  
Svenja Lohner ◽  
Andreas Tiehm
Keyword(s):  
Vinyl Chloride ◽  
Natural Attenuation ◽  
Enrichment Culture ◽  
Contaminated Sites ◽  
Contaminated Groundwater ◽  
Chlorinated Ethene ◽  
Equimolar Concentration ◽  
Bacterial Enrichment ◽  
Ph Tolerance

Degradation of the lower chlorinated ethenes is crucial to the application of natural attenuation or in situ bioremediation on chlorinated ethene contaminated sites. Recently, within mixtures of several chloroethenes as they can occur in contaminated groundwater inhibiting effects on aerobic chloroethene degradation have been shown. The current study demonstrated that metabolic vinyl chloride (VC) degradation by an enrichment culture originating from groundwater was not affected by an equimolar concentration (50 μM) of cis-1,2-dichloroethene (cDCE). Only cDCE concentrations at a ratio of 2.4:1 (initial cDCE to VC concentration) caused minor inhibition of VC degradation. Furthermore, the degradation of VC was not affected by the presence of trans-1,2-dichloroethene (tDCE), 1,1-dichloroethene (1,1-DCE), trichloroethene (TCE), and tetrachloroethene (PCE) in equimolar concentrations (50 μM). Only cDCE and tDCE were cometabolically degraded in small amounts. The VC-degrading culture demonstrated a broad pH tolerance from 5 to 9 with an optimum between 6 and 7. Results also showed that the culture could degrade VC concentrations up to 1,800 μM (110 mg/L).

On-site and in situ bioremediation of wood-preservative contaminated groundwater

2000 ◽  
Vol 42 (5-6) ◽  
pp. 371-376 ◽  
Author(s):  
J.A. Puhakka ◽  
K.T. Järvinen ◽  
J.H. Langwaldt ◽  
E.S. Melin ◽  
M.K. Männistö ◽  
...  
Keyword(s):  
Iron Oxidation ◽  
Wood Preservative ◽  
Pilot Scale ◽  
High Rate ◽  
Contaminated Groundwater ◽  
Groundwater Temperature ◽  
In Situ Bioremediation ◽  
Contaminated Aquifer ◽  
Fluidized Bed Process

This paper reviews ten years of research on on-site and in situ bioremediation of chlorophenol contaminated groundwater. Laboratory experiments on the development of a high-rate, fluidized-bed process resulted in a full-scale, pump-and-treat application which has operated for several years. The system operates at ambient groundwater temperature of 7 to 9°C at 2.7 d hydraulic retention time and chlorophenol removal efficiencies of 98.5 to 99.9%. The microbial ecology studies of the contaminated aquifer revealed a diverse chlorophenol-degrading community. In situ biodegradation of chlorophenols is controlled by oxygen availability, only. Laboratory and pilot-scale experiments showed the potential for in situ aquifer bioremediation with iron oxidation and precipitation as a potential problem.

scholarly journals Potential forMethanosarcinato contribute to uranium reduction during acetate-promoted groundwater bioremediation

2017 ◽  
Author(s):  
Dawn E Holmes ◽  
Roberto Orelana ◽  
Ludovic Giloteaux ◽  
Li-Ying Wang ◽  
Pravin Shrestha ◽  
...  
Keyword(s):  
Field Experiment ◽  
Electron Donor ◽  
Enrichment Culture ◽  
Cell Suspensions ◽  
Contaminated Groundwater ◽  
Subunit A ◽  
Sedimentary Environments

AbstractPrevious studies ofin situbioremediation of uranium-contaminated groundwater with acetate injections have focused on the role ofGeobacterspecies in U(VI) reduction because of a lack of other abundant known U(VI)-reducing microorganisms. Monitoring the levels of methyl CoM reductase subunit A (mcrA) transcripts during an acetate-injection field experiment demonstrated that acetoclastic methanogens from the genusMethanosarcinawere enriched after 40 days of acetate amendment. The increased abundance ofMethanosarcinacorresponded with an accumulation of methane in the groundwater. An enrichment culture dominated by aMethanosarcinaspecies with the sameMethanosarcina mcrAsequence that predominated in the field experiment could effectively convert acetate to methane. In order to determine whetherMethanosarcinaspecies could be participating in U(VI) reduction in the subsurface, cell suspensions ofM. barkeriwere incubated in the presence of U(VI) with acetate provided as the electron donor. U(VI) was reduced by metabolically activeM. barkericells, however, no U(VI) reduction was observed in inactive controls. These results demonstrate thatMethanosarcinaspecies could play an important role in the long-term bioremediation of uranium-contaminated aquifers after depletion of Fe(III) oxides limits the growth ofGeobacterspecies. The results also suggest thatMethanosarcinahave the potential to influence uranium geochemistry in a diversity of anaerobic sedimentary environments.

scholarly journals Feasibility study of in-situ bioremediation for nitrobenzene-contaminated groundwater

2017 ◽  
Vol 17 (4) ◽  
pp. 1160-1167 ◽  
Author(s):  
Na Liu ◽  
Yue Wang ◽  
Yonglei An ◽  
Feng Ding ◽  
Xiaolong Yu ◽  
...  
Keyword(s):  
Simulation Experiment ◽  
Laboratory Simulation ◽  
Contaminated Groundwater ◽  
Site Conditions ◽  
In Situ Bioremediation ◽  
Degrading Bacterium ◽  
Bacterium Strain ◽  
Negative Factors ◽  
Sufficient Oxygen

Although many studies have simulated in-situ bioremediation of contaminated groundwater, most of them have not considered hydrochemical conditions and indigenous microorganisms, thus potentially rendering results inapplicable to actual in-situ groundwater bioremediation projects. This study focused on a nitrobenzene-contaminated groundwater site located in Jilin City, China. The actual nitrobenzene-contaminated groundwater was taken from Jilin City to simulate in-situ groundwater bioremediation in the laboratory. The feasibility of in-situ bioremediation for nitrobenzene-contaminated groundwater was studied according to actual site conditions and characteristics of nitrobenzene-degrading microorganisms in groundwater. The results showed that nitrobenzene-degrading bacterium strain NB1 was the dominant species that could effectively and rapidly degrade nitrobenzene by a partial reductive pathway. No negative factors on the growth or degrading function of this strain in groundwater could be detected. During a laboratory simulation experiment, combined in-situ bioremediation technologies, namely air sparging and bioaugmentation, could readily remove approximately 89.56% of nitrobenzene from groundwater without adding nutrients; oxygen was found to be the important growth factor for strain NB1. As the substrate of nitroreductase, encoded by the nitrobenzene nitroreductase (nbzA) gene, nitrobenzene was likely to significantly affect the expression of this gene. In conclusion, in-situ bioremediation is a feasible way to solve the problem of nitrobenzene-contaminated groundwater in Jilin City as long as sufficient oxygen and biomass of strain NB1 is ensured.

Application of in situ chemical oxidation to remediate sulfolane-contaminated groundwater: batch and pilot-scale studies

2021 ◽  
Vol 223 ◽  
pp. 136-145
Author(s):  
Zong-Han Yang ◽  
Ya-Lei Chen ◽  
Francis Verpoort ◽  
Cheng-Di Dong ◽  
Chiu-Wen Chen ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Chemical Oxidation ◽  
Contaminated Groundwater ◽  
In Situ Chemical Oxidation
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