Field-Release of a Genetically Engineered Microorganism for Subsurface Soil Bioremediation Process Monitoring Control

1999 ◽  
Author(s):  
Y. Ahn ◽  
C. Werner ◽  
J. Easter ◽  
J. Jarrell
Keyword(s):  
Process Monitoring ◽  
Genetically Engineered ◽  
Soil Bioremediation ◽  
Field Release ◽  
Subsurface Soil ◽  
Genetically Engineered Microorganism

Field release of genetically-engineered Rhizobium meliloti and Bradyrhizobium japonicum strains

1990 ◽  
pp. 397-403 ◽  
Author(s):  
C. W. Ronson ◽  
A. Bosworth ◽  
M. Genova ◽  
S. Gudbrandsen ◽  
T. Hankinson ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Rhizobium Meliloti ◽  
Genetically Engineered ◽  
Field Release

scholarly journals Open Field Release of Genetically Engineered Sterile Male Aedes aegypti in Malaysia

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e42771 ◽  
Author(s):  
Renaud Lacroix ◽  
Andrew R. McKemey ◽  
Norzahira Raduan ◽  
Lim Kwee Wee ◽  
Wong Hong Ming ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Open Field ◽  
Genetically Engineered ◽  
Sterile Male ◽  
Field Release

Detection of Rhizobium meliloti cells in field soil and nodules by polymerase chain reaction

1995 ◽  
Vol 41 (9) ◽  
pp. 816-825 ◽  
Author(s):  
R. J. Watson ◽  
C. Haitas-Crockett ◽  
T. Martin ◽  
R. Heys
Keyword(s):  
Polymerase Chain Reaction ◽  
Rhizobium Meliloti ◽  
Genetically Engineered ◽  
Chain Reaction ◽  
Pcr Inhibitor ◽  
Vertical Location ◽  
Meliloti Strain ◽  
Identification Of Bacteria ◽  
Polymerase Chain ◽  
Genetically Engineered Microorganism

A genetically marked Rhizobium meliloti strain, R692, was prepared by insertion of a 1.7-kb DNA segment from Tn903 between the nifHDK and fixABC genes in the nod megaplasmid. This DNA was used as a marker, detectable by polymerase chain reaction (PCR), for the specific identification of bacteria in soil samples and alfalfa nodules. This detection technique was tested by applying different titres of the marked strain to field plots seeded with alfalfa. Samples of soil and nodules were assayed for the presence of the marker DNA fragment by PCR using primers specific to the marker sequence. The experiments revealed that the bacteria could be detected directly in soil containing about 103–104 bacteria/g, but greater sensitivity was prevented by potent PCR inhibitors present in the samples. The titre of the bacteria in the soil decreased rapidly after inoculation, dropping about 10-fold per week. Tests of vertical location of the bacteria in soil cores showed that the bacteria were initially dispersed to a depth of 18 cm, and subsequently retained viability in the top 2–8 cm. As few as 10 marked R. meliloti per gram of soil resulted in its establishment at detectable levels in nodules. Application of about 104–105 bacteria/g soil was sufficient to give the maximum number of nodules per plant and resulted in 70–90% occupancy by the marked strain. Limited movement of the inoculant was detected by analysis of nodules from plants adjacent to the sites where the bacteria were applied, probably by movement in water. The experiments demonstrated the advantages of PCR for the monitoring of marked microorganisms in the environment.Key words: genetically engineered microorganism, PCR inhibitor, nitrogen fixation, nif and fix genes, genetic marker.

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