scholarly journals Cloning and sequencing of the trpE gene from Arthrobacter globiformis ATCC 8010 and several related subsurface Arthrobacter isolates

1998 ◽  
Author(s):  
T. Chernova ◽  
V.K. Viswanathan ◽  
N. Austria ◽  
B.P. Nichols
Keyword(s):  
Arthrobacter Globiformis ◽  
Cloning And Sequencing

Monitoring the marine environment for small round structured viruses (SRSVS): a new approach to combating the transmission of these viruses by molluscan shellfish

1998 ◽  
Vol 38 (12) ◽  
pp. 51-56 ◽  
Author(s):  
K. Henshilwood ◽  
J. Green ◽  
D. N. Lees
Keyword(s):  
Enteric Virus ◽  
Winter Period ◽  
Rt Pcr ◽  
Cloning And Sequencing ◽  
New Approach ◽  
Human Enteric Virus ◽  
Different Strains ◽  
The Uk ◽  
Public Health Protection

This study investigates human enteric virus contamination of a shellfish harvesting area. Samples were analysed over a 14-month period for Small Round Structured Viruses (SRSVs) using a previously developed nested RT-PCR. A clear seasonal difference was observed with the largest numbers of positive samples obtained during the winter period (October to March). This data concurs with the known winter association of gastroenteric illness due to oyster consumption in the UK and also with the majority of the outbreaks associated with shellfish harvested from this area during the study period. RT-PCR positive amplicons were further characterised by cloning and sequencing. Sequence analysis of the positive samples identified eleven SRSV strains, of both Genogroup I and Genogroup II, occurring throughout the study period. Many shellfish samples contained a mixture of strains with a few samples containing up to three different strains with both Genogroups represented. The observed common occurrence of strain mixtures may have implications for the role of shellfish as a vector for dissemination of SRSV strains. These results show that nested RT-PCR can identify SRSV contamination in shellfish harvesting areas. Virus monitoring of shellfish harvesting areas by specialist laboratories using RT-PCR is a possible approach to combating the transmission of SRSVs by molluscan shellfish and could potentially offer significantly enhanced levels of public health protection.

Rapid communication: cloning and sequencing of a canine beta 2-adrenergic receptor cDNA.

1996 ◽  
Vol 74 (9) ◽  
pp. 2285
Author(s):  
C W Emala ◽  
J Kuhl ◽  
C A Hirshman ◽  
M A Levine
Keyword(s):  
Adrenergic Receptor ◽  
Cloning And Sequencing ◽  
Rapid Communication ◽  
Receptor Cdna ◽  
Beta 2

scholarly journals Cloning and sequencing of the cDNA for a 13th different subunit (IHQ) of beef heart cytochrome c oxidase

1989 ◽  
Vol 264 (28) ◽  
pp. 16858-16861
Author(s):  
R Lightowlers ◽  
S Takamiya ◽  
R Wessling ◽  
M Lindorfer ◽  
R A Capaldi
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Beef Heart ◽  
Cloning And Sequencing

scholarly journals Rat apolipoprotein E mRNA. Cloning and sequencing of double-stranded cDNA.

1983 ◽  
Vol 258 (14) ◽  
pp. 8993-9000 ◽  
Author(s):  
J W McLean ◽  
C Fukazawa ◽  
J M Taylor
Keyword(s):  
Apolipoprotein E ◽  
Cloning And Sequencing

scholarly journals DEER and RIDME Measurements of the Nitroxide-Spin Labelled Copper-Bound Amine Oxidase Homodimer from Arthrobacter Globiformis

2021 ◽  
Author(s):  
Hannah Russell ◽  
Rachel Stewart ◽  
Christopher Prior ◽  
Vasily S. Oganesyan ◽  
Thembaninkosi G. Gaule ◽  
...  
Keyword(s):  
Dipolar Coupling ◽  
Amine Oxidase ◽  
Spin Labels ◽  
Arthrobacter Globiformis ◽  
Copper Amine Oxidase ◽  
Dipole Interactions ◽  
Nitroxide Spin Labels ◽  
Double Electron Electron Resonance ◽  
Dipolar Modulation ◽  
Paramagnetic Centres

AbstractIn the study of biological structures, pulse dipolar spectroscopy (PDS) is used to elucidate spin–spin distances at nanometre-scale by measuring dipole–dipole interactions between paramagnetic centres. The PDS methods of Double Electron Electron Resonance (DEER) and Relaxation Induced Dipolar Modulation Enhancement (RIDME) are employed, and their results compared, for the measurement of the dipolar coupling between nitroxide spin labels and copper-II (Cu(II)) paramagnetic centres within the copper amine oxidase from Arthrobacter globiformis (AGAO). The distance distribution results obtained indicate that two distinct distances can be measured, with the longer of these at c.a. 5 nm. Conditions for optimising the RIDME experiment such that it may outperform DEER for these long distances are discussed. Modelling methods are used to show that the distances obtained after data analysis are consistent with the structure of AGAO.

Sign in / Sign up

Export Citation Format

Share Document