scholarly journals (Structure and function of the self-incompatibility proteins of Brassica oleracea): Progress report

1989 ◽  
Author(s):  
Not Given Author
2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.


2012 ◽  
Vol 472-475 ◽  
pp. 3384-3389
Author(s):  
Zai Qiang Huo ◽  
Xue Qun Zhu

It is valuable to be researched in the application of science of complexity to the forest ecosystem. Forest ecosystem is an adaptive complex system which is suggested to be at the edge of chaos or at the criticality. The inner interaction of a forest ecosystem is the main driving force for the self-organization, complexity and order in the forest ecosystem. Forest ecosystem complexity is one of the research frontiers of ecological and evolutionary problems presently. The application of science of complexity to the forest ecosystem complexity studies, its concept, background, methodology and theory are briefly introduced. The forest ecosystem complexity is defined as the structure and function diversity, self-organization and the order of an ecosystem. Its main methods include the cellular automaton, genetic algorithm, game theory, complex network, etc. This paper has discussed mechanism and development of forest ecosystem complexity, by applying the principle and methods of science of complexity, which is a new approach for understanding ecological and evolutionary problems.


2007 ◽  
Vol 23 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Brian Dolan

✓Anatomical and physiological understandings of the structure and function of the brain have worked to establish it as the “seat of the soul.” As an organ of reflection, meditation, and memory, the brain becomes synonymous with what defines the “self” through the existence of consciousness—of mind. Thus, the brain has been associated with a range of transcendent concepts—the soul, spirit, mind, and consciousness—that all relate in fundamental ways to each other both in terms of their perceived location within the brain and because of the way each works ultimately to define the person to whom the brain belongs. In this article, the author provides a brief exploration of how interrelated these categories have been when seen in the context of ancient, Renaissance, early modern, and modern philosophical and medical concerns; how the brain has variously been perceived as home to these intimate states of being; and how practitioners from the neurosciences have reflected on these questions. The author provides novel insights into the interrelationships of philosophy, theology, and medicine by examining these issues through the lens of the history of neuroscience.


1997 ◽  
Vol 9 (11) ◽  
pp. 2065-2076 ◽  
Author(s):  
M Pastuglia ◽  
V Ruffio-Châble ◽  
V Delorme ◽  
T Gaude ◽  
C Dumas ◽  
...  

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